 |
|||||
|
|||||
|
|||||
|
|||||
|
|||||
Previous Article | Next Article
The Journal of Neuroscience, February 15, 2001, 21(4):1247-1256
Ca2+-Independent Protein Kinase C Apl II Mediates the Serotonin-Induced Facilitation at Depressed Aplysia Sensorimotor Synapses
Frédéric Manseau1, Xiaotang Fan2, Tina Hueftlein2, Wayne S. Sossin2, and Vincent F. Castellucci1 1 Laboratoire de Neurobiologie et Comportement, Centre de Recherche en Sciences Neurologiques, Département de Physiologie, Université de Montréal, Montréal, Canada H3C 3J7, and 2 Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montréal, Canada H3A 2B4
At nondepressed Aplysia sensory to motor synapses, serotonin (5-HT) facilitates transmitter release primarily through a protein kinase A pathway. In contrast, at depressed Aplysia sensory to motor synapses, 5-HT facilitates transmitter release primarily through a protein kinase C (PKC)-dependent pathway. It is known that only two phorbol ester-activated PKC isoforms, the Ca2+-dependent PKC Apl I and the Ca2+-independent PKC Apl II, exist in the Aplysia nervous system. For the first time, we have now been able to functionally determine which isoform of PKC is involved in a particular form of plasticity. We microinjected cultured sensorimotor pairs of neurons with various PKC constructs tagged with the enhanced green fluorescent protein as a reporter for successful plasmid expression. Our results demonstrate that short-term facilitation of depressed synapses is mediated by PKC Apl II. Dominant-negative PKC Apl II, but not dominant-negative PKC Apl I, disrupted the normal kinetics of 5-HT-induced facilitation by completely blocking its rapid onset. This effect was specific to depressed synapses, because dominant-negative PKC Apl II did not inhibit 5-HT-mediated facilitation of nondepressed synapses. Our results suggest that not only different signal transduction pathways but also different isoforms of a specific cascade may mediate physiological responses according to the state of a synapse.
Key words: Aplysia; protein kinase C; memory; synaptic facilitation; serotonin; plasmids
Copyright © 2001 Society for Neuroscience 0270-6474/01/2141247-10$05.00/0
This article has been cited by other articles:
C. A. Farah, D. Weatherill, T. W. Dunn, and W. S. Sossin
PKC Differentially Translocates during Spaced and Massed Training in Aplysia
J. Neurosci., August 19, 2009; 29(33): 10281 - 10286.
[Abstract] [Full Text] [PDF]
G. Villareal, Q. Li, D. Cai, A. E. Fink, T. Lim, J. K. Bougie, W. S. Sossin, and D. L. Glanzman
Role of Protein Kinase C in the Induction and Maintenance of Serotonin-Dependent Enhancement of the Glutamate Response in Isolated Siphon Motor Neurons of Aplysia californica
J. Neurosci., April 22, 2009; 29(16): 5100 - 5107.
[Abstract] [Full Text] [PDF]
A. Khoutorsky and M. E. Spira
Activity-dependent calpain activation plays a critical role in synaptic facilitation and post-tetanic potentiation
Learn. Mem., January 29, 2009; 16(2): 129 - 141.
[Abstract] [Full Text] [PDF]
C. A. Farah, I. Nagakura, D. Weatherill, X. Fan, and W. S. Sossin
Physiological Role for Phosphatidic Acid in the Translocation of the Novel Protein Kinase C Apl II in Aplysia Neurons
Mol. Cell. Biol., August 1, 2008; 28(15): 4719 - 4733.
[Abstract] [Full Text] [PDF]
D. Fioravante, R.-Y. Liu, A. K. Netek, L. J. Cleary, and J. H. Byrne
Synapsin Regulates Basal Synaptic Strength, Synaptic Depression, and Serotonin-Induced Facilitation of Sensorimotor Synapses in Aplysia
J Neurophysiol, December 1, 2007; 98(6): 3568 - 3580.
[Abstract] [Full Text] [PDF]
J.-Y. Hu, Y. Chen, and S. Schacher
Protein Kinase C Regulates Local Synthesis and Secretion of a Neuropeptide Required for Activity-Dependent Long-Term Synaptic Plasticity
J. Neurosci., August 15, 2007; 27(33): 8927 - 8939.
[Abstract] [Full Text] [PDF]
W. S. Sossin
Isoform specificity of protein kinase Cs in synaptic plasticity
Learn. Mem., April 2, 2007; 14(4): 236 - 246.
[Abstract] [Full Text] [PDF]
M. Carroll, J. Dyer, and W. S. Sossin
Serotonin Increases Phosphorylation of Synaptic 4EBP through TOR, but Eukaryotic Initiation Factor 4E Levels Do Not Limit Somatic Cap-Dependent Translation in Aplysia Neurons
Mol. Cell. Biol., November 15, 2006; 26(22): 8586 - 8598.
[Abstract] [Full Text] [PDF]
Y. Zhao, K. Leal, C. Abi-Farah, K. C. Martin, W. S. Sossin, and M. Klein
Isoform Specificity of PKC Translocation in Living Aplysia Sensory Neurons and a Role for Ca2+-Dependent PKC APL I in the Induction of Intermediate-Term Facilitation.
J. Neurosci., August 23, 2006; 26(34): 8847 - 8856.
[Abstract] [Full Text] [PDF]
B. Dumitriu, J. E. Cohen, Q. Wan, A. M. Negroiu, and T. W. Abrams
Serotonin Receptor Antagonists Discriminate Between PKA- and PKC-Mediated Plasticity in Aplysia Sensory Neurons
J Neurophysiol, April 1, 2006; 95(4): 2713 - 2720.
[Abstract] [Full Text] [PDF]
S. Logsdon, A. F. M. Johnstone, K. Viele, and R. L. Cooper
Regulation of synaptic vesicles pools within motor nerve terminals during short-term facilitation and neuromodulation
J Appl Physiol, February 1, 2006; 100(2): 662 - 671.
[Abstract] [Full Text] [PDF]
J.-Y. Hu, F. Wu, and S. Schacher
Two Signaling Pathways Regulate the Expression and Secretion of a Neuropeptide Required for Long-Term Facilitation in Aplysia
J. Neurosci., January 18, 2006; 26(3): 1026 - 1035.
[Abstract] [Full Text] [PDF]
N. S. Magoski and L. K. Kaczmarek
Association/Dissociation of a Channel-Kinase Complex Underlies State-Dependent Modulation
J. Neurosci., August 31, 2005; 25(35): 8037 - 8047.
[Abstract] [Full Text] [PDF]
A. Khoutorsky and M. E. Spira
Calcium-activated proteases are critical for refilling depleted vesicle stores in cultured sensory-motor synapses of Aplysia
Learn. Mem., July 1, 2005; 12(4): 414 - 422.
[Abstract] [Full Text] [PDF]
Q. Li, A. C. Roberts, and D. L. Glanzman
Synaptic Facilitation and Behavioral Dishabituation in Aplysia: Dependence on Release of Ca2+ from Postsynaptic Intracellular Stores, Postsynaptic Exocytosis, and Modulation of Postsynaptic AMPA Receptor Efficacy
J. Neurosci., June 8, 2005; 25(23): 5623 - 5637.
[Abstract] [Full Text] [PDF]
F. Fiumara, S. Giovedi, A. Menegon, C. Milanese, D. Merlo, P. G. Montarolo, F. Valtorta, F. Benfenati, and M. Ghirardi
Phosphorylation by cAMP-dependent protein kinase is essential for synapsin-induced enhancement of neurotransmitter release in invertebrate neurons
J. Cell Sci., October 1, 2004; 117(21): 5145 - 5154.
[Abstract] [Full Text] [PDF]
D. Barbas, L. DesGroseillers, V. F. Castellucci, T. J. Carew, and S. Marinesco
Multiple Serotonergic Mechanisms Contributing to Sensitization in Aplysia: Evidence of Diverse Serotonin Receptor Subtypes
Learn. Mem., September 1, 2003; 10(5): 373 - 386.
[Abstract] [Full Text] [PDF]
A. Nakhost, G. Houeland, V. F. Castellucci, and W. S. Sossin
Differential Regulation of Transmitter Release by Alternatively Spliced Forms of Synaptotagmin I
J. Neurosci., July 16, 2003; 23(15): 6238 - 6244.
[Abstract] [Full Text] [PDF]
J. E. Cohen, C. U. Onyike, V. L. McElroy, A. H. Lin, and T. W. Abrams
Pharmacological Characterization of an Adenylyl Cyclase-Coupled 5-HT Receptor in Aplysia: Comparison With Mammalian 5-HT Receptors
J Neurophysiol, March 1, 2003; 89(3): 1440 - 1455.
[Abstract] [Full Text] [PDF]
A. M. Pepio, G. L. Thibault, and W. S. Sossin
Phosphoinositide-dependent Kinase Phosphorylation of Protein Kinase C Apl II Increases during Intermediate Facilitation in Aplysia
J. Biol. Chem., September 27, 2002; 277(40): 37116 - 37123.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|