 |
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
The Journal of Neuroscience, March 1, 2001, 21(5):1590-1599
Regulation of Mouse
Opioid Receptor Gene Expression by Retinoids
Jing Bi, Xinli Hu, Horace H. Loh, and Li-Na Wei Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455
The effect of retinoids on the expression of
opioid receptor (KOR) gene was examined in normal and transgenic animals. KOR-lacZ transgene expression was specifically elevated in KOR-positive areas of the developing CNS by depleting vitamin A from animal diets. The endogenous KOR mRNA species, including all three isoforms, were also upregulated by depleting vitamin A in developing animals. Change in the expression of isoforms a and b is similar in prenatal stages but differs during postnatal development. Interestingly, upregulation of isoform c is most significant postnatally. The regulation of KOR gene by vitamin A was substantiated in a mouse embryonal carcinoma P19 culture system in which retinoic acid (RA), the most potent ingredient of vitamin A, was able to suppress the expression of all the three KOR isoforms and KOR protein. The RA-mediated suppression was blocked by an RA receptor antagonist and a histone deacetylase (HDAC) inhibitor. By using a reporter transfection assay in P19 cells, the potential genetic element responsible for RA-mediated suppression of KOR gene expression was located to intron 1 of the mouse KOR gene, which could also be blocked by HDAC inhibitor. Furthermore, suppression of KOR gene expression by RA in P19 cells appeared to be an indirect event and required protein synthesis. A role of RA in KOR gene regulation during developmental stages was discussed.
Key words:
Copyright © 2001 Society for Neuroscience 0270-6474/01/2151590-10$05.00/0
This article has been cited by other articles:
W. S. Sheng, S. Hu, G. Herr, H. T. Ni, R. B. Rock, G. Gekker, J. R. Lokensgard, and P. K. Peterson
Human Neural Precursor Cells Express Functional {kappa}-Opioid Receptors
J. Pharmacol. Exp. Ther., September 1, 2007; 322(3): 957 - 963.
[Abstract] [Full Text] [PDF]
J. Bi, N.-P. Tsai, Y.-P. Lin, H. H. Loh, and L.-N. Wei
Axonal mRNA transport and localized translational regulation of {kappa}-opioid receptor in primary neurons of dorsal root ganglia
PNAS, December 26, 2006; 103(52): 19919 - 19924.
[Abstract] [Full Text] [PDF]
E. Kim, A. L. Clark, A. Kiss, J. W. Hahn, R. Wesselschmidt, C. J. Coscia, and M. M. Belcheva
{micro}- and {kappa}-Opioids Induce the Differentiation of Embryonic Stem Cells to Neural Progenitors
J. Biol. Chem., November 3, 2006; 281(44): 33749 - 33760.
[Abstract] [Full Text] [PDF]
N.-P. Tsai, J. Bi, H. H. Loh, and L.-N. Wei
Netrin-1 signaling regulates de novo protein synthesis of kappa opioid receptor by facilitating polysomal partition of its mRNA.
J. Neurosci., September 20, 2006; 26(38): 9743 - 9749.
[Abstract] [Full Text] [PDF]
S. W. Park, M. D. M. Huq, H. H. Loh, and L.-N. Wei
Retinoic Acid-Induced Chromatin Remodeling of Mouse {kappa} Opioid Receptor Gene
J. Neurosci., March 30, 2005; 25(13): 3350 - 3357.
[Abstract] [Full Text] [PDF]
J. Bi, X. Hu, H. H. Loh, and L.-N. Wei
Mouse {kappa}-Opioid Receptor mRNA Differential Transport in Neurons
Mol. Pharmacol., September 1, 2003; 64(3): 594 - 599.
[Abstract] [Full Text] [PDF]
J. Li, S. W. Park, H. H. Loh, and L.-N. Wei
Induction of the Mouse kappa -Opioid Receptor Gene by Retinoic Acid in P19 Cells
J. Biol. Chem., October 11, 2002; 277(42): 39967 - 39972.
[Abstract] [Full Text] [PDF]
X. Hu, J. Bi, H. H. Loh, and L.-N. Wei
Regulation of Mouse kappa Opioid Receptor Gene Expression by Different 3'-Untranslated Regions and the Effect of Retinoic Acid
Mol. Pharmacol., October 1, 2002; 62(4): 881 - 887.
[Abstract] [Full Text] [PDF]
S. W. Park, J. Li, H. H. Loh, and L.-N. Wei
A Novel Signaling Pathway of Nitric Oxide on Transcriptional Regulation of Mouse kappa Opioid Receptor Gene
J. Neurosci., September 15, 2002; 22(18): 7941 - 7947.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|