QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (18) Citing Articles via Google Scholar Google Scholar Articles by Stimson, D. T. Articles by Ramaswami, M. Search for Related Content PubMed PubMed Citation Articles by Stimson, D. T. Articles by Ramaswami, M.

 Previous Article  |  Next Article 

The Journal of Neuroscience, May 1, 2001, 21(9):3034-3044

Drosophila Stoned Proteins Regulate the Rate and Fidelity of Synaptic Vesicle Internalization

Daniel T. Stimson¹, Patricia S. Estes¹, Sujata Rao³, K. S. Krishnan³, Leonard E. Kelly², and Mani Ramaswami¹

¹ Department of Molecular and Cellular Biology and Arizona Research Laboratories Division of Neurobiology, University of Arizona, Tucson, Arizona 85721, ² Department of Genetics, University of Melbourne, Parkville, Australia, and ³ Department of Biological Sciences, Tata Institute of Fundamental Research, Bombay, India

At an initial step during synaptic vesicle recycling, dynamin and adaptor proteins mediate the endocytosis of synaptic vesicle components from the plasma membrane. StonedA and stonedB, novel synaptic proteins encoded by a single Drosophila gene, have predicted structural similarities to adaptors and other proteins implicated in endocytosis. Here, we test possible roles of the stoned proteins in synaptic vesicle internalization via analyses of third instar larval neuromuscular synapses in two Drosophila stoned (stn) mutants, stn^ts and stn^8P1. Both mutations reduce presynaptic levels of stonedA and stonedB, although stn^ts has relatively weak effects. The mutations cause retention of synaptic vesicle proteins on the presynaptic plasma membrane but do not alter the levels or distribution of endocytosis proteins, dynamin, -adaptin, and clathrin. In addition, stn^8P1 mutants exhibit depletion and enlargement of synaptic vesicles. To determine whether these defects arise from altered synaptic vesicle endocytosis or from defects in synaptic vesicle biogenesis, we implemented new methods to assess directly the efficiency of synaptic vesicle recycling and membrane internalization at Drosophila nerve terminals. Behavioral and electrophysiological analyses indicate that stn^ts, an allele with normal evoked release and synaptic vesicle number, enhances defects in synaptic vesicle recycling shown by Drosophila shi^ts mutants. A dye uptake assay demonstrates that slow synaptic vesicle recycling in stn^ts is accompanied by a reduced rate of synaptic vesicle internalization after exocytosis. These observations are consistent with a model in which stonedA and stonedB act to facilitate the internalization of synaptic vesicle components from the plasma membrane.

Key words: stonedA; stonedB; adaptins; shibire; dynamin; larval neuromuscular junction

---

Copyright © 2001 Society for Neuroscience  0270-6474/01/2193034-11$05.00/0

This article has been cited by other articles:

				N. Jung, M. Wienisch, M. Gu, J. B. Rand, S. L. Muller, G. Krause, E. M. Jorgensen, J. Klingauf, and V. Haucke Molecular basis of synaptic vesicle cargo recognition by the endocytic sorting adaptor stonin 2 J. Cell Biol., December 31, 2007; 179(7): 1497 - 1510. [Abstract] [Full Text] [PDF]

				C. A. Loewen, S.-M. Lee, Y.-K. Shin, and N. E. Reist C2B Polylysine Motif of Synaptotagmin Facilitates a Ca2+-independent Stage of Synaptic Vesicle Priming In Vivo Mol. Biol. Cell, December 1, 2006; 17(12): 5211 - 5226. [Abstract] [Full Text] [PDF]

				H. Bao, R. W. Daniels, G. T. MacLeod, M. P. Charlton, H. L. Atwood, and B. Zhang AP180 Maintains the Distribution of Synaptic and Vesicle Proteins in the Nerve Terminal and Indirectly Regulates the Efficacy of Ca2+-Triggered Exocytosis J Neurophysiol, September 1, 2005; 94(3): 1888 - 1903. [Abstract] [Full Text] [PDF]

				A. A. Wall, A. M. Phillips, and L. E. Kelly Effective Translation of the Second Cistron in Two Drosophila Dicistronic Transcripts Is Determined by the Absence of In-frame AUG Codons in the First Cistron J. Biol. Chem., July 29, 2005; 280(30): 27670 - 27678. [Abstract] [Full Text] [PDF]

				P. S. Estes, T. C. Jackson, D. T. Stimson, S. Sanyal, L. E. Kelly, and M. Ramaswami Functional Dissection of a Eukaryotic Dicistronic Gene: Transgenic stonedB, but Not stonedA, Restores Normal Synaptic Properties to Drosophila stoned Mutants Genetics, September 1, 2003; 165(1): 185 - 196. [Abstract] [Full Text] [PDF]

				R. Rikhy, V. Kumar, R. Mittal, and K. S. Krishnan Endophilin Is Critically Required for Synapse Formation and Function in Drosophila melanogaster J. Neurosci., September 1, 2002; 22(17): 7478 - 7484. [Abstract] [Full Text] [PDF]

				M. Boehm and J. S. Bonifacino Adaptins. The Final Recount Mol. Biol. Cell, October 1, 2001; 12(10): 2907 - 2920. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help