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The Journal of Neuroscience, June 1, 2002, 22(11):4388-4398

Bidirectional Alterations in Cerebellar Synaptic Transmission of tottering and rolling Ca²⁺ Channel Mutant Mice

Kaori Matsushita^{1, 4}, Minoru Wakamori^{1, 5}, Im Joo Rhyu⁶, Tatsuo Arii^{2, 4}, Sen-ichi Oda⁷, Yasuo Mori^{3, 4}, and Keiji Imoto^{1, 4}

¹ Department of Information Physiology and ² Center for Brain Experiment, National Institute for Physiological Sciences, ³ Center for Integrative Bioscience, Okazaki National Research Institutes, and ⁴ School of Life Science, the Graduate University for Advanced Studies, Okazaki 444-8585, Japan, ⁵ Department of Physiology, Kagoshima University Faculty of Medicine, Kagoshima 890-8520, Japan, ⁶ Department of Anatomy, Korea University College of Medicine, Seoul 136-705, Korea, and ⁷ Laboratory of Animal Management, School of Agricultural Sciences, Nagoya University, Nagoya, Aichi 464-8601, Japan

Hereditary ataxic mice, tottering (tg) and rolling Nagoya (tg^rol), carry mutations in the P/Q-type Ca²⁺ channel _1A subunit gene. The positions of the mutations and the neurological phenotypes are known, but the mechanisms of how the mutations cause the symptoms and how the different mutations lead to various onset and severity have remained unsolved. Here we compared fundamental properties of excitatory synaptic transmission in the cerebellum and roles of Ca²⁺ channel subtypes therein among wild-type control, tg, and tg^rol mice. The amplitude of EPSC of the parallel fiber-Purkinje cell (PF-PC) synapses was considerably reduced in ataxic tg^rol. Although the amplitude of the parallel fiber-mediated EPSC was only mildly decreased in young non-ataxic tg mice, it was drastically diminished in adult ataxic tg mice of postnatal day 28-35, showing a good correlation between the impairment of the PF-PC synaptic transmission and manifestation of ataxia. In contrast, the EPSC amplitude of the climbing fiber-Purkinje cell (CF-PC) synapses was preserved in tg, and it was even increased in tg^rol, which was associated with altered properties of the postsynaptic glutamate receptors. The climbing fiber-mediated EPSC was more dependent on other Ca²⁺ channel subtypes in mutant mice, suggesting that such compensatory mechanisms contribute to maintaining the CF-PC synaptic transmission virtually intact. The results indicate that different mutations of the P/Q-type Ca²⁺ channel not only cause the primary effect of different severity but also lead to diverse additional secondary effects, resulting in disruption of well balanced neural networks.

Key words: calcium channel; synaptic transmission; mutant mice; tottering mice; rolling Nagoya mice; cerebellar ataxia; glutamate receptor

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Copyright © 2002 Society for Neuroscience  0270-6474/02/22114388-11$05.00/0

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