 |
|||||
|
|||||
|
|||||
|
|||||
|
|||||
Previous Article | Next Article
The Journal of Neuroscience, September 1, 2002, 22(17):7797-7808
Identification and Characterization of the Feeding Circuit-Activating Peptides, a Novel Neuropeptide Family of Aplysia
J. V. Sweedler1, L. Li1, S. S. Rubakhin1, V. Alexeeva2, N. C. Dembrow2, O. Dowling2, J. Jing2, K. R. Weiss2, and F. S. Vilim2 1 Department of Chemistry and Beckman Institute, University of Illinois, Urbana, Illinois 61801, and 2 Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, New York 10029
We use a multidisciplinary approach to identify, map, and characterize the bioactivity of modulatory neuropeptides in the circuitry that generates feeding behavior in Aplysia. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of the cerebral-buccal connective (CBC), a nerve containing axons of many interneurons that control feeding behavior of Aplysia, was used to identify neuropeptides that may participate in generation and shaping of feeding motor programs. Using this functionally oriented search, we identified a novel family of peptides that we call the feeding circuit-activating peptides (FCAPs). Two peptides with masses identical to those observed in the CBCs (molecular weight 1387 and 1433) were purified from buccal ganglia and partially sequenced using mass spectrometry. The amino acid sequence was then used to clone the FCAP precursor, which encodes multiple copies of eight different FCAPs. The two FCAPs present in highest copy number correspond to those observed in the CBC. The distribution of FCAP expression was mapped using Northern analysis, whole-mount in situ hybridization, and immunocytochemistry. Consistent with our initial findings, FCAP-immunopositive axons were observed in the CBC. Furthermore, we found that FCAP was present in some cerebral-buccal and buccal-cerebral interneurons. As their name suggests, FCAPs are capable of initiating rhythmic feeding motor programs and are the first neuropeptides with such activity in this circuit. The actions of FCAPs suggest that these peptides may contribute to the induction and maintenance of food-induced arousal. FCAPs were also localized to several other neuronal systems, suggesting that FCAPs may play a role in the regulation of multiple behaviors.
Key words: MALDI-TOF MS; Aplysia californica; cDNA cloning; neuropeptide processing; in situ hybridization; immunocytochemistry; feeding behavior
Copyright © 2002 Society for Neuroscience 0270-6474/02/22177797-12$05.00/0
This article has been cited by other articles:
Y. Sugiura, Y. Konishi, N. Zaima, S. Kajihara, H. Nakanishi, R. Taguchi, and M. Setou
Visualization of the cell-selective distribution of PUFA-containing phosphatidylcholines in mouse brain by imaging mass spectrometry
J. Lipid Res., September 1, 2009; 50(9): 1776 - 1788.
[Abstract] [Full Text] [PDF]
J. Jing, F. S. Vilim, E. C. Cropper, and K. R. Weiss
Neural Analog of Arousal: Persistent Conditional Activation of a Feeding Modulator by Serotonergic Initiators of Locomotion
J. Neurosci., November 19, 2008; 28(47): 12349 - 12361.
[Abstract] [Full Text] [PDF]
J. Jing, F. S. Vilim, C. C. Horn, V. Alexeeva, N. G. Hatcher, K. Sasaki, I. Yashina, Y. Zhurov, I. Kupfermann, J. V. Sweedler, et al.
From Hunger to Satiety: Reconfiguration of a Feeding Network by Aplysia Neuropeptide Y
J. Neurosci., March 28, 2007; 27(13): 3490 - 3502.
[Abstract] [Full Text] [PDF]
R. Gillette
Evolution and Function in Serotonergic Systems
Integr. Comp. Biol., December 1, 2006; 46(6): 838 - 846.
[Abstract] [Full Text] [PDF]
M. Diaz-Rios and M. W. Miller
Target-Specific Regulation of Synaptic Efficacy in the Feeding Central Pattern Generator of Aplysia: Potential Substrates for Behavioral Plasticity?
Biol. Bull., June 1, 2006; 210(3): 215 - 229.
[Abstract] [Full Text] [PDF]
A. Proekt, F. S. Vilim, V. Alexeeva, V. Brezina, A. Friedman, J. Jing, L. Li, Y. Zhurov, J. V. Sweedler, and K. R. Weiss
Identification of a New Neuropeptide Precursor Reveals a Novel Source of Extrinsic Modulation in the Feeding System of Aplysia
J. Neurosci., October 19, 2005; 25(42): 9637 - 9648.
[Abstract] [Full Text] [PDF]
H.-Y. Koh and K. R. Weiss
Peptidergic Contribution to Posttetanic Potentiation at a Central Synapse of Aplysia
J Neurophysiol, August 1, 2005; 94(2): 1281 - 1286.
[Abstract] [Full Text] [PDF]
S. L. Hooper
Variation Is the Spice of Life. Focus on "Cycle-to-Cycle Variability of Neuromuscular Activity in Aplysia Feeding Behavior"
J Neurophysiol, July 1, 2004; 92(1): 40 - 41.
[Full Text] [PDF]
N. C. Dembrow, J. Jing, A. Proekt, A. Romero, F. S. Vilim, E. C. Cropper, and K. R. Weiss
A Newly Identified Buccal Interneuron Initiates and Modulates Feeding Motor Programs in Aplysia
J Neurophysiol, October 1, 2003; 90(4): 2190 - 2204.
[Abstract] [Full Text] [PDF]
H.-Y. Koh, F. S. Vilim, J. Jing, and K. R. Weiss
Two Neuropeptides Colocalized in a Command-Like Neuron Use Distinct Mechanisms to Enhance Its Fast Synaptic Connection
J Neurophysiol, September 1, 2003; 90(3): 2074 - 2079.
[Abstract] [Full Text] [PDF]
Y. Furukawa, K. Nakamaru, K. Sasaki, Y. Fujisawa, H. Minakata, S. Ohta, F. Morishita, O. Matsushima, L. Li, V. Alexeeva, et al.
PRQFVamide, a Novel Pentapeptide Identified From the CNS and Gut of Aplysia
J Neurophysiol, June 1, 2003; 89(6): 3114 - 3127.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|