QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (20) Citing Articles via Google Scholar Google Scholar Articles by Müller, U. Articles by Hildebrandt, H. Search for Related Content PubMed PubMed Citation Articles by Müller, U. Articles by Hildebrandt, H.

 Previous Article  |  Next Article 

The Journal of Neuroscience, October 1, 2002, 22(19):8739-8747

Nitric Oxide/cGMP-Mediated Protein Kinase A Activation in the Antennal Lobes Plays an Important Role in Appetitive Reflex Habituation in the Honeybee

Uli Müller¹ and Herbert Hildebrandt²

¹ Institut für Biologie der Freien Universität Berlin, Neurobiologie, 14195 Berlin, Germany, and ² Institut für Zoologie der Universität Stuttgart-Hohenheim, 70593 Stuttgart, Germany

Habituation, a form of non-associative learning, is observed throughout the animal kingdom. However, in contrast to associative learning, little is known about the underlying molecular mechanisms. Using the appetitive proboscis extension reflex in honeybees, we show that the cAMP-dependent protein kinase A (PKA) in the antennal lobe (AL) is implicated in the graded decline of behavioral response during habituation. Repeated stimulation leads to a slow and gradual increase in PKA activity superimposed on a fast transient PKA activation induced by each stimulus. These temporally distinct components of PKA activation are pharmacologically dissectible and are restricted to the AL on the stimulated side. Whereas the transient PKA activation induced by each stimulus requires monoaminergic transmission, the slow component of PKA activation is mediated by the nitric oxide (NO)/cGMP system. Local manipulation of the slow component of PKA activation in single ALs specifically interferes with the dynamic of habituation on the corresponding side. Our results provide strong evidence that NO/cGMP-mediated PKA activation in each AL contributes to temporal signal integration during habituation. Dishabituation by a sensory stimulus or spontaneous recovery from habituation does not require the PKA cascade. This provides evidence that the mechanisms underlying dishabituation and spontaneous recovery differ from those underlying temporal signal integration during habituation of the proboscis extension response.

Key words: antennal lobes; cAMP-dependent protein kinase; dishabituation; habituation; insect; plasticity; recovery from habituation; second messenger

---

Copyright © 2002 Society for Neuroscience  0270-6474/02/22198739-09$05.00/0

This article has been cited by other articles:

				T. Yabumoto, F. Takanashi, Y. Kirino, and S. Watanabe Nitric oxide is involved in appetitive but not aversive olfactory learning in the land mollusk Limax valentianus Learn. Mem., April 2, 2008; 15(4): 229 - 232. [Abstract] [Full Text] [PDF]

				I. T. Barbarossa, P. Muroni, M. D. Setzu, and A. M. Angioy Dose-Dependent Nonassociative Olfactory Learning in a Fly Chem Senses, July 1, 2007; 32(6): 535 - 541. [Abstract] [Full Text] [PDF]

				R. Menzel and G. Manz Neural plasticity of mushroom body-extrinsic neurons in the honeybee brain J. Exp. Biol., November 15, 2005; 208(22): 4317 - 4332. [Abstract] [Full Text] [PDF]

				K. C. Daly, T. A. Christensen, H. Lei, B. H. Smith, and J. G. Hildebrand Learning modulates the ensemble representations for odors in primary olfactory networks PNAS, July 13, 2004; 101(28): 10476 - 10481. [Abstract] [Full Text] [PDF]

				A. Friedrich, U. Thomas, and U. Muller Learning at Different Satiation Levels Reveals Parallel Functions for the cAMP-Protein Kinase A Cascade in Formation of Long-Term Memory J. Neurosci., May 5, 2004; 24(18): 4460 - 4468. [Abstract] [Full Text] [PDF]

				R. Scheiner, M. B. Sokolowski, and J. Erber Activity of cGMP-Dependent Protein Kinase (PKG) Affects Sucrose Responsiveness and Habituation in Drosophila melanogaster Learn. Mem., May 1, 2004; 11(3): 303 - 311. [Abstract] [Full Text] [PDF]

				A. J. Susswein, A. Katzoff, N. Miller, and I. Hurwitz Nitric Oxide and Memory Neuroscientist, April 1, 2004; 10(2): 153 - 162. [Abstract] [PDF]

				Y. Ben-Shahar, H.-T. Leung, W. L. Pak, M. B. Sokolowski, and G. E. Robinson cGMP-dependent changes in phototaxis: a possible role for the foraging gene in honey bee division of labor J. Exp. Biol., July 15, 2003; 206(14): 2507 - 2515. [Abstract] [Full Text] [PDF]

				T. Farooqui, K. Robinson, H. Vaessin, and B. H. Smith Modulation of Early Olfactory Processing by an Octopaminergic Reinforcement Pathway in the Honeybee J. Neurosci., June 15, 2003; 23(12): 5370 - 5380. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help