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The Journal of Neuroscience, January 15, 2002, 22(2):471-477
Antagonistic Effects of Rnd1 and RhoD GTPases Regulate Receptor Activity in Semaphorin 3A-Induced Cytoskeletal Collapse
Silvio M. Zanata1, 3, Iiris Hovatta1, Beate Rohm1, and Andreas W. Püschel1, 2 1 Molecular Neurogenetics Laboratory, Department of Neurochemistry, Max-Planck-Institute for Brain Research, D-60528 Frankfurt, Germany, 2 Institut für Allgemeine Zoologie und Genetik, Westfälische Wilhelms-Universität, D-48149 Münster, Germany, and 3 Ludwig Institute for Cancer Research, Cellular and Molecular Biology Laboratory, 01509-010 Sao Paulo, Brazil
The semaphorins are a large protein family that is involved in the patterning of neuronal connections in the developing nervous system of both vertebrates and invertebrates. The chemorepulsive axon guidance signal Semaphorin 3A (Sema3A) induces the depolymerization of actin filaments and the collapse of sensory growth cones by activating a receptor complex that contains a plexin as the signal-transducing subunit. Here we show that, of a large number of GTPases tested, only Rnd1 and RhoD bind the cytoplasmic domain of Plexin-A1. Recruitment of active Rnd1 is sufficient to trigger signaling by Plexin-A1, even in the absence of Sema3A, and initiates cytoskeletal collapse by activating its cytoplasmic domain. RhoD, in contrast, blocks Plexin-A1 activation by Rnd1 and repulsion of sympathetic axons by Sema3A. Thus, the antagonism of two GTPases regulates the activity of the Sema3A receptor, and activation by Rnd1 appears to be an essential step in signaling by Plexin-A1.
Key words: GTPases; cytoskeleton; neuropilin; plexin; actin; semaphorin
Copyright © 2002 Society for Neuroscience 0270-6474/02/222471-07$05.00/0
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