QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (22) Citing Articles via Google Scholar Google Scholar Articles by Rodan, A. R. Articles by Heberlein, U. Search for Related Content PubMed PubMed Citation Articles by Rodan, A. R. Articles by Heberlein, U.

 Previous Article  |  Next Article 

The Journal of Neuroscience, November 1, 2002, 22(21):9490-9501

Functional Dissection of Neuroanatomical Loci Regulating Ethanol Sensitivity in Drosophila

Aylin R. Rodan^{1, 3}, John A. Kiger Jr⁴, and Ulrike Heberlein^{1, 2, 3}

¹ Department of Anatomy and Programs in ² Neuroscience and ³ Biological Sciences, University of California, San Francisco, California 94143-0452, and ⁴ Department of Molecular and Cellular Biology, University of California, Davis, California 95616

Ethanol has complex but similar effects on behavior in mammals and the fruit fly Drosophila melanogaster. In addition, genetic and pharmacological approaches have implicated the cAMP pathway in the regulation of ethanol-induced behaviors in both flies and rodents. Here we examine the neuroanatomical loci that modulate ethanol sensitivity in Drosophila by targeting the expression of an inhibitor of cAMP-dependent protein kinase (PKA) to specific regions in the fly's brain. Expression of the inhibitor in most brain regions or in muscle has no effect on behavior. In contrast, inhibition of PKA in a relatively small number of cells, possibly neurosecretory cells, in the fly's brain is sufficient to decrease sensitivity to the incoordinating effects of ethanol. Additional brain areas are, however, also involved. The mushroom bodies, brain structures where cAMP signaling is required for olfactory classical conditioning, are dispensable for the regulation of ethanol sensitivity. Finally, different behavioral effects of ethanol, motor incoordination and sedation, appear to be regulated by PKA function in distinct brain regions. We conclude that the regulation of ethanol-induced behaviors by PKA involves complex interactions among groups of cells that mediate either increased or reduced sensitivity to the acute intoxicating effects of ethanol.

Key words: Drosophila; ethanol; PKA; neuroanatomy; mushroom bodies; postural control; locomotion

---

Copyright © 2002 Society for Neuroscience  0270-6474/02/22219490-12$05.00/0

This article has been cited by other articles:

				A. Crocker and A. Sehgal Octopamine Regulates Sleep in Drosophila through Protein Kinase A-Dependent Mechanisms J. Neurosci., September 17, 2008; 28(38): 9377 - 9385. [Abstract] [Full Text] [PDF]

				J. H. Bauer, C. Chang, S. N. S. Morris, S. Hozier, S. Andersen, J. S. Waitzman, and S. L. Helfand Expression of dominant-negative Dmp53 in the adult fly brain inhibits insulin signaling PNAS, August 14, 2007; 104(33): 13355 - 13360. [Abstract] [Full Text] [PDF]

				N. L. Urizar, Z. Yang, H. J. Edenberg, and R. L. Davis Drosophila Homer Is Required in a Small Set of Neurons Including the Ellipsoid Body for Normal Ethanol Sensitivity and Tolerance J. Neurosci., April 25, 2007; 27(17): 4541 - 4551. [Abstract] [Full Text] [PDF]

				H. Luan, W. C. Lemon, N. C. Peabody, J. B. Pohl, P. K. Zelensky, D. Wang, M. N. Nitabach, T. C. Holmes, and B. H. White Functional Dissection of a Neuronal Network Required for Cuticle Tanning and Wing Expansion in Drosophila J. Neurosci., January 11, 2006; 26(2): 573 - 584. [Abstract] [Full Text] [PDF]

				U. Heberlein, F. W. Wolf, A. Rothenfluh, and D. J. Guarnieri Molecular Genetic Analysis of Ethanol Intoxication in Drosophila melanogaster Integr. Comp. Biol., August 1, 2004; 44(4): 269 - 274. [Abstract] [Full Text] [PDF]

				F. W. Wolf, A. R. Rodan, L. T.-Y. Tsai, and U. Heberlein High-Resolution Analysis of Ethanol-Induced Locomotor Stimulation in Drosophila J. Neurosci., December 15, 2002; 22(24): 11035 - 11044. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help