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The Journal of Neuroscience, December 15, 2002, 22(24):10524-10528

BRIEF COMMUNICATION
Presynaptic Ca²⁺ Entry Is Unchanged during Hippocampal Mossy Fiber Long-Term Potentiation

Haruyuki Kamiya^{1, 2}, Kazumasa Umeda^{1, 3}, Seiji Ozawa^{2, 4}, and Toshiya Manabe^{1, 5}

¹ Division of Cell Biology and Neurophysiology, Department of Neuroscience, Faculty of Medicine, Kobe University, Kobe, Hyogo 650-0017, Japan, ² Department of Physiology, Gunma University School of Medicine, Maebashi, Gunma 371-8511, Japan, ³ Department of Applied Biology, Kyoto Institute of Technology, Kyoto 606-8585, Japan, ⁴ Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan, and ⁵ Division of Neuronal Network, Department of Basic Medical Sciences, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan

The hippocampal mossy fiber (MF)-CA3 synapse exhibits NMDA receptor-independent long-term potentiation (LTP), which is expressed by presynaptic mechanisms leading to persistent enhancement of transmitter release. Recent studies have identified several molecules that may play an important role in MF-LTP. These include Rab3A, RIM1, kainate autoreceptor, and hyperpolarization-activated cation channel (I_h). However, the precise cellular expression mechanism remains to be determined because some studies noticed essential roles of release machinery molecules, whereas others suggested modulation of the ionotropic processes affecting Ca²⁺ entry into the presynaptic terminals. Using fluorescence recordings of presynaptic Ca²⁺ in hippocampal slices, here we demonstrated that MF-LTP is not accompanied by an increase in presynaptic Ca²⁺ influx during an action potential. Whole-cell recordings from CA3 neurons revealed long-lasting increases in mean frequency, but not mean amplitude, of miniature EPSCs after the high-frequency stimulation of MFs. These data indicate that the presynaptic expression mechanisms responsible for enhanced transmitter release during MF-LTP involve persistent modification of presynaptic molecular targets residing downstream of Ca²⁺ entry.

Key words: cAMP; hippocampus; long-term potentiation; mossy fiber; presynaptic Ca²⁺ influx; transmitter release

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Copyright © 2002 Society for Neuroscience  0270-6474/02/222410524-05$05.00/0

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