WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (71)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Klein, C.
Right arrow Articles by Trotter, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Klein, C.
Right arrow Articles by Trotter, J.

 Previous Article  |  Next Article 

The Journal of Neuroscience, February 1, 2002, 22(3):698-707

Process Outgrowth of Oligodendrocytes Is Promoted by Interaction of Fyn Kinase with the Cytoskeletal Protein Tau

Corinna Klein1, Eva-Maria Krämer1, Anne-Marie Cardine2, Burkhardt Schraven2, Roland Brandt1, and Jacqueline Trotter1

Departments of 1 Neurobiology and 2 Immunology, University of Heidelberg, 69120 Heidelberg, Germany

Fyn kinase plays an important role during myelination and has been shown to promote morphological differentiation of cultured oligodendrocytes. We analyzed the downstream targets of Fyn kinase in oligodendrocytes. Because process outgrowth and wrapping of axons involve cytoskeletal rearrangement, we focused on cytoskeletal proteins linked to Fyn. Here we demonstrate that Fyn binds to the cytoskeletal proteins Tau and alpha -Tubulin in oligodendrocytes. Tau interacts with the Fyn SH3 domain whereas alpha -Tubulin binds to the Fyn SH2 and SH3 domains. To study the function of the Fyn-Tau interaction in oligodendrocytes, we designed a Tau deletion mutant that would compete with endogenous Tau-Fyn binding in transfected cells. The mutant Tau protein binds to the Fyn SH3 domain but lacks the microtubuli interaction domain and thus cannot bind to microtubuli. In the presence of the mutant Tau protein, a reduction of the process number and process length in oligodendroglial cells was observed. This effect is likely to be caused by interference with the Fyn-Tau-microtubuli cascade rather than inactivation of the kinase, because Fyn bound to the mutant Tau retains activity. A similar inhibition of process outgrowth was observed when oliogodendroglial cells were cultured in the presence of Fumonisin B1, an inhibitor of sphingolipid synthesis that prevents the formation of rafts. Because ligation of the cell adhesion molecule F3 on oligodendrocytes leads to activation of Fyn kinase localized in rafts, these findings suggest that recruitment of Tau and Tubulin to activated Fyn kinase in rafts is an important step in the initiation of myelination.

Key words: myelination; oligodendrocytes; Fyn kinase; cytoskeleton; Tau; glycosphingolipid-rich rafts

Copyright © 2002 Society for Neuroscience  0270-6474/02/223698-10$05.00/0


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
P.-S. Wang, J. Wang, Z.-C. Xiao, and C. J. Pallen
Protein-tyrosine Phosphatase {alpha} Acts as an Upstream Regulator of Fyn Signaling to Promote Oligodendrocyte Differentiation and Myelination
J. Biol. Chem., November 27, 2009; 284(48): 33692 - 33702.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
R. White, C. Gonsior, E.-M. Kramer-Albers, N. Stohr, S. Huttelmaier, and J. Trotter
Activation of oligodendroglial Fyn kinase enhances translation of mRNAs transported in hnRNP A2-dependent RNA granules
J. Cell Biol., October 17, 2008; 181(4): 579 - 586.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
Y. Miyamoto, J. Yamauchi, and A. Tanoue
Cdk5 Phosphorylation of WAVE2 Regulates Oligodendrocyte Precursor Cell Migration through Nonreceptor Tyrosine Kinase Fyn
J. Neurosci., August 13, 2008; 28(33): 8326 - 8337.
[Abstract] [Full Text] [PDF]

Home page
 BrainHome page
M. Dubois-Dalcq, A. Williams, C. Stadelmann, B. Stankoff, B. Zalc, and C. Lubetzki
From fish to man: understanding endogenous remyelination in central nervous system demyelinating diseases
Brain, July 1, 2008; 131(7): 1686 - 1700.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. H. Reynolds, C. J. Garwood, S. Wray, C. Price, S. Kellie, T. Perera, M. Zvelebil, A. Yang, P. W. Sheppard, I. M. Varndell, et al.
Phosphorylation Regulates Tau Interactions with Src Homology 3 Domains of Phosphatidylinositol 3-Kinase, Phospholipase C{gamma}1, Grb2, and Src Family Kinases
J. Biol. Chem., June 27, 2008; 283(26): 18177 - 18186.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
R. Williamson, A. Usardi, D. P. Hanger, and B. H. Anderton
Membrane-bound {beta}-amyloid oligomers are recruited into lipid rafts by a fyn-dependent mechanism
FASEB J, May 1, 2008; 22(5): 1552 - 1559.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
D. Beacham, M. Ahn, W. A. Catterall, and T. Scheuer
Sites and Molecular Mechanisms of Modulation of NaV1.2 Channels by Fyn Tyrosine Kinase
J. Neurosci., October 24, 2007; 27(43): 11543 - 11551.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
H. B. Werner, K. Kuhlmann, S. Shen, M. Uecker, A. Schardt, K. Dimova, F. Orfaniotou, A. Dhaunchak, B. G. Brinkmann, W. Mobius, et al.
Proteolipid Protein Is Required for Transport of Sirtuin 2 into CNS Myelin
J. Neurosci., July 18, 2007; 27(29): 7717 - 7730.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. N. Fewou, H. Ramakrishnan, H. Bussow, V. Gieselmann, and M. Eckhardt
Down-regulation of Polysialic Acid Is Required for Efficient Myelin Formation
J. Biol. Chem., June 1, 2007; 282(22): 16700 - 16711.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
J. Lee, M. Gravel, R. Zhang, P. Thibault, and P. E. Braun
Process outgrowth in oligodendrocytes is mediated by CNP, a novel microtubule assembly myelin protein
J. Cell Biol., August 15, 2005; 170(4): 661 - 673.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
P. Derkinderen, T. M. E. Scales, D. P. Hanger, K.-Y. Leung, H. L. Byers, M. A. Ward, C. Lenz, C. Price, I. N. Bird, T. Perera, et al.
Tyrosine 394 Is Phosphorylated in Alzheimer's Paired Helical Filament Tau and in Fetal Tau with c-Abl as the Candidate Tyrosine Kinase
J. Neurosci., July 13, 2005; 25(28): 6584 - 6593.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. P. Zamora-Leon, A. Bresnick, J. M. Backer, and B. Shafit-Zagardo
Fyn Phosphorylates Human MAP-2c on Tyrosine 67
J. Biol. Chem., January 21, 2005; 280(3): 1962 - 1970.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Z. Lu, L. Ku, Y. Chen, and Y. Feng
Developmental Abnormalities of Myelin Basic Protein Expression in fyn Knock-out Brain Reveal a Role of Fyn in Posttranscriptional Regulation
J. Biol. Chem., January 7, 2005; 280(1): 389 - 395.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C. E. Laurent, F. J. Delfino, H. Y. Cheng, and T. E. Smithgall
The Human c-Fes Tyrosine Kinase Binds Tubulin and Microtubules through Separate Domains and Promotes Microtubule Assembly
Mol. Cell. Biol., November 1, 2004; 24(21): 9351 - 9358.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
X. Liang, N. A. Draghi, and M. D. Resh
Signaling from Integrins to Fyn to Rho Family GTPases Regulates Morphologic Differentiation of Oligodendrocytes
J. Neurosci., August 11, 2004; 24(32): 7140 - 7149.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
T. Kawarabayashi, M. Shoji, L. H. Younkin, L. Wen-Lang, D. W. Dickson, T. Murakami, E. Matsubara, K. Abe, K. H. Ashe, and S. G. Younkin
Dimeric Amyloid {beta} Protein Rapidly Accumulates in Lipid Rafts followed by Apolipoprotein E and Phosphorylated Tau Accumulation in the Tg2576 Mouse Model of Alzheimer's Disease
J. Neurosci., April 14, 2004; 24(15): 3801 - 3809.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
L. Decker and C. ffrench-Constant
Lipid Rafts and Integrin Activation Regulate Oligodendrocyte Survival
J. Neurosci., April 14, 2004; 24(15): 3816 - 3825.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
J. AVILA, J. J. LUCAS, M. PEREZ, and F. HERNANDEZ
Role of Tau Protein in Both Physiological and Pathological Conditions
Physiol Rev, April 1, 2004; 84(2): 361 - 384.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
G. Lee, R. Thangavel, V. M. Sharma, J. M. Litersky, K. Bhaskar, S. M. Fang, L. H. Do, A. Andreadis, G. Van Hoesen, and H. Ksiezak-Reding
Phosphorylation of Tau by Fyn: Implications for Alzheimer's Disease
J. Neurosci., March 3, 2004; 24(9): 2304 - 2312.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
N. Setterblad, C. Roucard, C. Bocaccio, J.-P. Abastado, D. Charron, and N. Mooney
Composition of MHC class II-enriched lipid microdomains is modified during maturation of primary dendritic cells
J. Leukoc. Biol., July 1, 2003; 74(1): 40 - 48.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Stegmuller, H. Werner, K.-A. Nave, and J. Trotter
The Proteoglycan NG2 Is Complexed with alpha -Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptors by the PDZ Glutamate Receptor Interaction Protein (GRIP) in Glial Progenitor Cells. IMPLICATIONS FOR GLIAL-NEURONAL SIGNALING
J. Biol. Chem., January 31, 2003; 278(6): 3590 - 3598.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J.-H. Cho and G. V. W. Johnson
Glycogen Synthase Kinase 3beta Phosphorylates Tau at Both Primed and Unprimed Sites. DIFFERENTIAL IMPACT ON MICROTUBULE BINDING
J. Biol. Chem., January 3, 2003; 278(1): 187 - 193.
[Abstract] [Full Text] [PDF]


-
-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help
-
Copyright 2009 by Society for Neuroscience ONLINE ISSN: 1529-2401
-