 |
 Previous Article | Next Article 
The Journal of Neuroscience, February 1, 2002, 22(3):803-814
Delphilin: a Novel PDZ and Formin Homology Domain-Containing
Protein that Synaptically Colocalizes and Interacts with Glutamate
Receptor  2 Subunit
Yohei
Miyagi1,
Tetsuji
Yamashita2,
Masahiro
Fukaya3,
Tomoko
Sonoda2,
Toshiaki
Okuno4,
Kazuyuki
Yamada3,
Masahiko
Watanabe3,
Yoji
Nagashima1,
Ichiro
Aoki1,
Kenji
Okuda2,
Masayoshi
Mishina4, 5, and
Susumu
Kawamoto2
Departments of 1 Pathology and
2 Bacteriology, Yokohama City University School of
Medicine, Yokohama 236-0004, Japan, 3 Department of
Anatomy, Hokkaido University School of Medicine, Sapporo 060-8638, Japan, 4 Department of Molecular Neurobiology and
Pharmacology, University of Tokyo Graduate School of Medicine, Tokyo
113-0033, Japan, and 5 Core Research for Evolutional
Science and Technology, Japan Science and Technology
Corporation, Saitama 332-0012, Japan
The glutamate receptor  2 (GluR2) subunit is selectively
expressed in cerebellar Purkinje cells and plays an important role in
cerebellar long-term depression, motor learning, motor coordination, and synapse development. We identified a novel GluR2-interacting protein, named Delphilin, that contains a single PDZ domain and formin
homology (FH) domains FH1 and FH2 plus coiled-coil structure. As far as
we know, this is the first reported protein that contains both PDZ and
FH domains. Yeast two-hybrid and surface plasmon resonance (SPR)
analyses indicated that Delphilin interacts with the GluR2 C
terminus via its PDZ domain. This was also supported by
coimmunoprecipitation experiments using a heterologous expression system in mammalian cells. Yeast cell and SPR analyses also
demonstrated the possibility that the FH1 proline-rich region of
Delphilin interacts with profilin, an actin-binding protein, and with
the Src homology 3 domain of neuronal Src protein tyrosine
kinase. In situ hybridization demonstrated the highest
expression of Delphilin mRNA in Purkinje cells. Delphilin polypeptide
was highly enriched in the synaptosomal membrane fraction of the
cerebellum and coimmunoprecipitated with the GluR2 subunit. The
post-embedding immunogold technique demonstrated that Delphilin is
selectively localized at the postsynaptic junction site of the parallel
fiber-Purkinje cell synapse and colocalized with GluR2. Thus,
Delphilin is a postsynaptic scaffolding protein at the parallel
fiber-Purkinje cell synapse, where it may serve to link GluR2 with
actin cytoskeleton and various signaling molecules.
Key words:
glutamate receptor 2 subunit; cerebellum; Purkinje
cell; parallel fiber synapses; yeast two-hybrid; surface plasmon
resonance; PDZ domain; FH domain; post-embedding immunogold
labeling
Copyright © 2002 Society for Neuroscience 0270-6474/02/223803-12$05.00/0
This article has been cited by other articles:
|
|
|
|
 
D. Chalkia, N. Nikolaidis, W. Makalowski, J. Klein, and M. Nei
Origins and Evolution of the Formin Multigene Family That Is Involved in the Formation of Actin Filaments
Mol. Biol. Evol.,
December 1, 2008;
25(12):
2717 - 2733.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Kakegawa, T. Miyazaki, K. Emi, K. Matsuda, K. Kohda, J. Motohashi, M. Mishina, S. Kawahara, M. Watanabe, and M. Yuzaki
Differential Regulation of Synaptic Plasticity and Cerebellar Motor Learning by the C-Terminal PDZ-Binding Motif of GluR{delta}2
J. Neurosci.,
February 6, 2008;
28(6):
1460 - 1468.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Uemura, S. Kakizawa, M. Yamasaki, K. Sakimura, M. Watanabe, M. Iino, and M. Mishina
Regulation of Long-Term Depression and Climbing Fiber Territory by Glutamate Receptor {delta}2 at Parallel Fiber Synapses through its C-Terminal Domain in Cerebellar Purkinje Cells
J. Neurosci.,
October 31, 2007;
27(44):
12096 - 12108.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Kakegawa, K. Kohda, and M. Yuzaki
The {delta}2 'ionotropic' glutamate receptor functions as a non-ionotropic receptor to control cerebellar synaptic plasticity
J. Physiol.,
October 1, 2007;
584(1):
89 - 96.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Matsuda, S. Matsuda, C. M. Gladding, and M. Yuzaki
Characterization of the {delta}2 Glutamate Receptor-binding Protein Delphilin: SPLICING VARIANTS WITH DIFFERENTIAL PALMITOYLATION AND AN ADDITIONAL PDZ DOMAIN
J. Biol. Chem.,
September 1, 2006;
281(35):
25577 - 25587.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Yawata, H. Tsuchida, M. Kengaku, and T. Hirano
Membrane-proximal region of glutamate receptor delta2 subunit is critical for long-term depression and interaction with protein interacting with C kinase 1 in a cerebellar Purkinje neuron.
J. Neurosci.,
April 5, 2006;
26(14):
3626 - 3633.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Takeuchi, T. Miyazaki, M. Watanabe, H. Mori, K. Sakimura, and M. Mishina
Control of Synaptic Connection by Glutamate Receptor {delta}2 in the Adult Cerebellum
J. Neurosci.,
February 23, 2005;
25(8):
2146 - 2156.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. N. Higgs and K. J. Peterson
Phylogenetic Analysis of the Formin Homology 2 Domain
Mol. Biol. Cell,
January 1, 2005;
16(1):
1 - 13.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Wang, J. R. Eldstrom, J. Jantzi, E. D. Moore, and D. Fedida
Increased focal Kv4.2 channel expression at the plasma membrane is the result of actin depolymerization
Am J Physiol Heart Circ Physiol,
February 1, 2004;
286(2):
H749 - H759.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Evangelista, S. Zigmond, and C. Boone
Formins: signaling effectors for assembly and polarization of actin filaments
J. Cell Sci.,
July 1, 2003;
116(13):
2603 - 2611.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Tabakoff, S. V. Bhave, and P. L. Hoffman
Selective Breeding, Quantitative Trait Locus Analysis, and Gene Arrays Identify Candidate Genes for Complex Drug-Related Behaviors
J. Neurosci.,
June 1, 2003;
23(11):
4491 - 4498.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Sekerkova, P. A. Loomis, B. Changyaleket, L. Zheng, R. Eytan, B. Chen, E. Mugnaini, and J. R. Bartles
Novel Espin Actin-Bundling Proteins Are Localized to Purkinje Cell Dendritic Spines and Bind the Src Homology 3 Adapter Protein Insulin Receptor Substrate p53
J. Neurosci.,
February 15, 2003;
23(4):
1310 - 1319.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
