 |
 Previous Article | Next Article 
The Journal of Neuroscience, February 15, 2002, 22(4):1228-1237
Activation of Central Terminal Vanilloid Receptor-1 Receptors and
  -Methylene-ATP-Sensitive P2X Receptors Reveals a Converged
Synaptic Activity onto the Deep Dorsal Horn Neurons of the Spinal
Cord
Terumasa
Nakatsuka1,
Hidemasa
Furue2,
Megumu
Yoshimura2, and
Jianguo G.
Gu1
1 McKnight Brain Institute of the University of Florida
and Division of Neuroscience, Department of Oral Surgery, College of
Dentistry, University of Florida, Gainesville, Florida 32610, and
2 Department of Integrative Physiology, Graduate School of
Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku,
Fukuoka, 812-8582 Japan
Using a spinal cord slice preparation and patch-clamp recordings
from spinal cord dorsal horn neurons, we examined excitatory and
inhibitory circuits connecting to lamina V neurons after the activation
of afferent central terminal vanilloid receptor-1 (VR1) receptors and
P2X receptors. We found that single neurons in lamina V often received
excitatory inputs from two chemically defined afferent pathways. One of
these pathways was polysynaptic from capsaicin-sensitive afferent
terminals. In this pathway the capsaicin-sensitive afferent input first
activated interneurons in superficial laminas, and then the
excitatory activity was transmitted onto lamina V neurons.
The second excitatory input was monosynaptic from
  m-ATP-sensitive/capsaicin-insensitive afferent terminals. Both
capsaicin-sensitive and m-ATP-sensitive/capsaicin-insensitive pathways also recruited polysynaptic inhibitory inputs to lamina V
neurons. Furthermore, we demonstrated that simultaneous activation of
both capsaicin-sensitive afferent pathways and
m-ATP-sensitive/capsaicin-insensitive pathways could generate a
temporal summation of excitatory inputs onto single lamina V neurons.
These convergent pathways may provide a mechanism of sensory
integration for two chemically defined sensory inputs and may have
implications in different sensory states.
Key words:
capsaicin; VR1 receptors; ATP; ,-methylene-ATP; P2X receptors; EPSCs; IPSCs; spinal cord slice
preparation
Copyright © 2002 Society for Neuroscience 0270-6474/02/2241228-10$05.00/0
This article has been cited by other articles:
|
|
|
|
 
Y. V. Medvedeva, M.-S. Kim, and Y. M. Usachev
Mechanisms of Prolonged Presynaptic Ca2+ Signaling and Glutamate Release Induced by TRPV1 Activation in Rat Sensory Neurons
J. Neurosci.,
May 14, 2008;
28(20):
5295 - 5311.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Sikand and L. S. Premkumar
Potentiation of glutamatergic synaptic transmission by protein kinase C-mediated sensitization of TRPV1 at the first sensory synapse
J. Physiol.,
June 1, 2007;
581(2):
631 - 647.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kosugi, T. Nakatsuka, T. Fujita, Y. Kuroda, and E. Kumamoto
Activation of TRPA1 Channel Facilitates Excitatory Synaptic Transmission in Substantia Gelatinosa Neurons of the Adult Rat Spinal Cord
J. Neurosci.,
April 18, 2007;
27(16):
4443 - 4451.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Burnstock
Physiology and Pathophysiology of Purinergic Neurotransmission
Physiol Rev,
April 1, 2007;
87(2):
659 - 797.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Shiokawa, T. Nakatsuka, H. Furue, M. Tsuda, T. Katafuchi, K. Inoue, and M. Yoshimura
Direct excitation of deep dorsal horn neurones in the rat spinal cord by the activation of postsynaptic P2X receptors
J. Physiol.,
June 15, 2006;
573(3):
753 - 763.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Raisinghani, R. M Pabbidi, and L. S Premkumar
Activation of transient receptor potential vanilloid 1 (TRPV1) by resiniferatoxin
J. Physiol.,
September 15, 2005;
567(3):
771 - 786.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Y. Chiang, S. Zhang, Y. F. Xie, J. W. Hu, J. O. Dostrovsky, M. W. Salter, and B. J. Sessle
Endogenous ATP Involvement in Mustard-Oil-Induced Central Sensitization in Trigeminal Subnucleus Caudalis (Medullary Dorsal Horn)
J Neurophysiol,
September 1, 2005;
94(3):
1751 - 1760.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-H. Jin, T. W. Bailey, B.-y. Li, J. H. Schild, and M. C. Andresen
Purinergic and Vanilloid Receptor Activation Releases Glutamate from Separate Cranial Afferent Terminals in Nucleus Tractus Solitarius
J. Neurosci.,
May 19, 2004;
24(20):
4709 - 4717.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. North
P2X3 receptors and peripheral pain mechanisms
J. Physiol.,
January 15, 2004;
554(2):
301 - 308.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. G. Gu
P2X Receptor-Mediated Modulation of Sensory Transmission to the Spinal Cord Dorsal Horn
Neuroscientist,
October 1, 2003;
9(5):
370 - 378.
[Abstract]
[PDF]
|
|
|
|
|
|
|
K. Tsuzuki, A. Ase, P. Seguela, T. Nakatsuka, C.-Y. Wang, J.-X. She, and J. G. Gu
TNP-ATP-Resistant P2X Ionic Current on the Central Terminals and Somata of Rat Primary Sensory Neurons
J Neurophysiol,
June 1, 2003;
89(6):
3235 - 3242.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Nakatsuka, K. Tsuzuki, J. X. Ling, H. Sonobe, and J. G. Gu
Distinct Roles of P2X Receptors in Modulating Glutamate Release at Different Primary Sensory Synapses in Rat Spinal Cord
J Neurophysiol,
June 1, 2003;
89(6):
3243 - 3252.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Marinelli, V. Di Marzo, N. Berretta, I. Matias, M. Maccarrone, G. Bernardi, and N. B. Mercuri
Presynaptic Facilitation of Glutamatergic Synapses to Dopaminergic Neurons of the Rat Substantia Nigra by Endogenous Stimulation of Vanilloid Receptors
J. Neurosci.,
April 15, 2003;
23(8):
3136 - 3144.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. W. Doyle, T. W. Bailey, Y.-H. Jin, and M. C. Andresen
Vanilloid Receptors Presynaptically Modulate Cranial Visceral Afferent Synaptic Transmission in Nucleus Tractus Solitarius
J. Neurosci.,
September 15, 2002;
22(18):
8222 - 8229.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
