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The Journal of Neuroscience, February 15, 2002, 22(4):1228-1237

Activation of Central Terminal Vanilloid Receptor-1 Receptors and -Methylene-ATP-Sensitive P2X Receptors Reveals a Converged Synaptic Activity onto the Deep Dorsal Horn Neurons of the Spinal Cord

Terumasa Nakatsuka¹, Hidemasa Furue², Megumu Yoshimura², and Jianguo G. Gu¹

¹ McKnight Brain Institute of the University of Florida and Division of Neuroscience, Department of Oral Surgery, College of Dentistry, University of Florida, Gainesville, Florida 32610, and ² Department of Integrative Physiology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582 Japan

Using a spinal cord slice preparation and patch-clamp recordings from spinal cord dorsal horn neurons, we examined excitatory and inhibitory circuits connecting to lamina V neurons after the activation of afferent central terminal vanilloid receptor-1 (VR1) receptors and P2X receptors. We found that single neurons in lamina V often received excitatory inputs from two chemically defined afferent pathways. One of these pathways was polysynaptic from capsaicin-sensitive afferent terminals. In this pathway the capsaicin-sensitive afferent input first activated interneurons in superficial laminas, and then the excitatory activity was transmitted onto lamina V neurons. The second excitatory input was monosynaptic from m-ATP-sensitive/capsaicin-insensitive afferent terminals. Both capsaicin-sensitive and m-ATP-sensitive/capsaicin-insensitive pathways also recruited polysynaptic inhibitory inputs to lamina V neurons. Furthermore, we demonstrated that simultaneous activation of both capsaicin-sensitive afferent pathways and m-ATP-sensitive/capsaicin-insensitive pathways could generate a temporal summation of excitatory inputs onto single lamina V neurons. These convergent pathways may provide a mechanism of sensory integration for two chemically defined sensory inputs and may have implications in different sensory states.

Key words: capsaicin; VR1 receptors; ATP; ,-methylene-ATP; P2X receptors; EPSCs; IPSCs; spinal cord slice preparation

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Copyright © 2002 Society for Neuroscience  0270-6474/02/2241228-10$05.00/0

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