QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (44) Citing Articles via Google Scholar Google Scholar Articles by Cho, H. Articles by Oh, U. Search for Related Content PubMed PubMed Citation Articles by Cho, H. Articles by Oh, U.

 Previous Article  |  Next Article 

The Journal of Neuroscience, February 15, 2002, 22(4):1238-1247

Mechanosensitive Ion Channels in Cultured Sensory Neurons of Neonatal Rats

Hawon Cho, Jieun Shin, Chan Young Shin, Soon-Youl Lee, and Uhtaek Oh

The Sensory Research Center, Creative Research Initiatives, College of Pharmacy, Seoul National University, Seoul 151-742, Korea

Mechanosensitive (MS) ion channels are present in a variety of cells. However, very little is known about the ion channels that account for mechanical sensitivity in sensory neurons. We identified the two most frequently encountered but distinct types of MS channels in 1390 of 2962 membrane patches tested in cultured dorsal root ganglion neurons. The two MS channels exhibited different thresholds, thus named as low-threshold (LT) and high-threshold (HT) MS channels, and sensitivity to pressure. The two channels retained different single-channel conductances and current-voltage relationships: LT and HT channels elicited large- and small-channel conductance with outwardly rectifying and linear I-V relationships, respectively. Both LT and HT MS channels were permeable to monovalent cations and Ca²⁺ and were blocked by gadolinium, a blocker of MS channels. Colchicine and cytochalasin D markedly reduced the activities of the two MS channels, indicating that cytoskeletal elements support the mechanosensitivity. Both types of MS channels were found primarily in small sensory neurons with diameters of <30 µm. Furthermore, HT MS channels were sensitized by a well known inducer of mechanical hyperalgesia, prostaglandin E₂, via the protein kinase A pathway. We identified two distinct types of MS channels in sensory neurons that probably give rise to the observed MS whole-cell currents and transduce mechanical stimuli to neural signals involved in somatosensation, including pain.

Key words: mechanosensitive channels; cationic; sensory neurons; somatosensation; pain; sensitization

---

Copyright © 2002 Society for Neuroscience  0270-6474/02/2241238-10$05.00/0

This article has been cited by other articles:

				S. G. Lechner and G. R. Lewin Peripheral sensitisation of nociceptors via G-proteindependent potentiation of mechanotransduction currents J. Physiol., July 15, 2009; 587(14): 3493 - 3503. [Abstract] [Full Text] [PDF]

				N. Alessandri-Haber, O. A. Dina, X. Chen, and J. D. Levine TRPC1 and TRPC6 Channels Cooperate with TRPV4 to Mediate Mechanical Hyperalgesia and Nociceptor Sensitization J. Neurosci., May 13, 2009; 29(19): 6217 - 6228. [Abstract] [Full Text] [PDF]

				T. Ishida, T. Yabushita, and K. Soma Effects of a Liquid Diet on Temporomandibular Joint Mechano-receptors Journal of Dental Research, February 1, 2009; 88(2): 187 - 191. [Abstract] [Full Text] [PDF]

				M. R. C. Bhattacharya, D. M. Bautista, K. Wu, H. Haeberle, E. A. Lumpkin, and D. Julius Radial stretch reveals distinct populations of mechanosensitive mammalian somatosensory neurons PNAS, December 16, 2008; 105(50): 20015 - 20020. [Abstract] [Full Text] [PDF]

				A. Gomis, S. Soriano, C. Belmonte, and F. Viana Hypoosmotic- and pressure-induced membrane stretch activate TRPC5 channels J. Physiol., December 1, 2008; 586(23): 5633 - 5649. [Abstract] [Full Text] [PDF]

				N. Milenkovic, C. Wetzel, R. Moshourab, and G. R. Lewin Speed and Temperature Dependences of Mechanotransduction in Afferent Fibers Recorded From the Mouse Saphenous Nerve J Neurophysiol, November 1, 2008; 100(5): 2771 - 2783. [Abstract] [Full Text] [PDF]

				J. H. Nam, H.-S. Lee, Y. H. Nguyen, T. M. Kang, S. W. Lee, H.-Y. Kim, S. J. Kim, Y. E. Earm, and S. J. Kim Mechanosensitive activation of K+ channel via phospholipase C-induced depletion of phosphatidylinositol 4,5-bisphosphate in B lymphocytes J. Physiol., August 1, 2007; 582(3): 977 - 990. [Abstract] [Full Text] [PDF]

				Z. Zhang, A. N. Kindrat, R. Sharif-Naeini, and C. W. Bourque Actin Filaments Mediate Mechanical Gating during Osmosensory Transduction in Rat Supraoptic Nucleus Neurons J. Neurosci., April 11, 2007; 27(15): 4008 - 4013. [Abstract] [Full Text] [PDF]

				J. Hu and G. R. Lewin Mechanosensitive currents in the neurites of cultured mouse sensory neurones J. Physiol., December 15, 2006; 577(3): 815 - 828. [Abstract] [Full Text] [PDF]

				B. J. Canning Reflex regulation of airway smooth muscle tone J Appl Physiol, September 1, 2006; 101(3): 971 - 985. [Abstract] [Full Text] [PDF]

				S. Ciura and C. W. Bourque Transient receptor potential vanilloid 1 is required for intrinsic osmoreception in organum vasculosum lamina terminalis neurons and for normal thirst responses to systemic hyperosmolality. J. Neurosci., August 30, 2006; 26(35): 9069 - 9075. [Abstract] [Full Text] [PDF]

				C. Roza, J.-L. Puel, M. Kress, A. Baron, S. Diochot, M. Lazdunski, and R. Waldmann Knockout of the ASIC2 channel in mice does not impair cutaneous mechanosensation, visceral mechanonociception and hearing J. Physiol., July 15, 2004; 558(2): 659 - 669. [Abstract] [Full Text] [PDF]

				J. Aleu, M. Martin-Satue, P. Navarro, I. P. de Lara, L. Bahima, J. Marsal, and C. Solsona Release of ATP induced by hypertonic solutions in Xenopus oocytes J. Physiol., February 15, 2003; 547(1): 209 - 219. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help