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The Journal of Neuroscience, February 15, 2002, 22(4):1290-1302

I_h Channels Contribute to the Different Functional Properties of Identified Dopaminergic Subpopulations in the Midbrain

Henrike Neuhoff^*, Axel Neu^*, Birgit Liss, and Jochen Roeper

Medical Research Council, Anatomical Neuropharmacology Unit, Department of Pharmacology, Oxford University, OX1 3TH United Kingdom

Dopaminergic (DA) midbrain neurons in the substantia nigra (SN) and ventral tegmental area (VTA) are involved in various brain functions such as voluntary movement and reward and are targets in disorders such as Parkinson's disease and schizophrenia. To study the functional properties of identified DA neurons in mouse midbrain slices, we combined patch-clamp recordings with either neurobiotin cell-filling and triple labeling confocal immunohistochemistry, or single-cell RT-PCR. We discriminated four DA subpopulations based on anatomical and neurochemical differences: two calbindin D₂₈-k (CB)-expressing DA populations in the substantia nigra (SN/CB+) or ventral tegmental area (VTA/CB+), and respectively, two calbindin D₂₈-k negative DA populations (SN/CB, VTA/CB). VTA/CB+ DA neurons displayed significantly faster pacemaker frequencies with smaller afterhyperpolarizations compared with other DA neurons. In contrast, all four DA populations possessed significant differences in I_h channel densities and I_h channel-mediated functional properties like sag amplitudes and rebound delays in the following order: SN/CB  VTA/CB  SN/CB+  VTA/CB+. Single-cell RT-multiplex PCR experiments demonstrated that differential calbindin but not calretinin expression is associated with differential I_h channel densities. Only in SN/CB DA neurons, however, I_h channels were actively involved in pacemaker frequency control. In conclusion, diversity within the DA system is not restricted to distinct axonal projections and differences in synaptic connectivity, but also involves differences in postsynaptic conductances between neurochemically and topographically distinct DA neurons.

Key words: HCN channels; dopamine; calbindin; substantia nigra; ventral tegmental area; pacemaker; Parkinson's disease; confocal immunohistochemistry; single-cell RT-PCR

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^* H.N. and A.N. contributed equally to this work.

Correspondence should be addressed to Dr. Jochen Roeper, Medical Research Council, Anatomical Neuropharmacology Unit, Oxford University, Mansfield Road, Oxford OX1 3TH, UK. E-mail: jochen.roeper{at}pharm.ox.ac.uk.

H. Neuhoff's present address: Scientific Services, Morphology, Zentrum für Molekulare Neurobiologie Hamburg, D-20251 Hamburg, Germany.

A. Neu's present address: Institute for Neural Signaltransduction, Zentrum für Molekulare Neurobiologie Hamburg, D-20251 Hamburg, Germany.

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Copyright © 2002 Society for Neuroscience  0270-6474/02/2241290-13$05.00/0

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