QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (27) Citing Articles via Google Scholar Google Scholar Articles by Pan, C.-Y. Articles by Fox, A. P. Search for Related Content PubMed PubMed Citation Articles by Pan, C.-Y. Articles by Fox, A. P.

 Previous Article  |  Next Article 

The Journal of Neuroscience, April 1, 2002, 22(7):2427-2433

Alterations in Exocytosis Induced by Neuronal Ca²⁺ Sensor-1 in Bovine Chromaffin Cells

Chien-Yuan Pan¹, Andreas Jeromin², Kenneth Lundstrom³, Seung Hyun Yoo⁴, John Roder², and Aaron P. Fox¹

¹ Department of Neurobiology, Pharmacology, and Physiology, The University of Chicago, Chicago, Illinois 60637, ² Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, M5G 1X5 Canada, ³ F. Hoffmann-La Roche, CNS Department, CH-4070 Basel, Switzerland, and ⁴ National Creative Research Initiative Center for Secretory Granule Research, Korean Advanced Institute of Science and Technology, Dae Jeon 305-701, Korea

A variety of Ca²⁺ binding proteins are known to play an integral role in catecholamine release from synapses as well as secretory cells, such as chromaffin cells. The Drosophila protein frequenin and its mammalian homolog neuronal Ca²⁺ sensor-1 (NCS-1) belong to a family of Ca²⁺ sensors with EF hands that bind Ca²⁺ and then interact with other proteins. Frequenin/NCS-1 has been shown to enhance exocytotic activity in addition to altering Ca²⁺ channel regulation. To better understand how NCS-1 regulates stimulus-secretion coupling, bovine chromaffin cells were infected with Semliki Forest virus (SFV) vectors containing the rat NCS-1 gene. Cells were studied in the perforated whole-cell patch-clamp configuration. Membrane capacitance was monitored as an indicator of exocytosis-endocytosis. Exocytosis elicited by membrane depolarization was not significantly different between cells infected with SFV expressing green fluorescent protein (GFP) or GFP plus NCS-1, except that the overexpression of NCS-1 resulted in a faster rundown in exocytosis. When cells were stimulated with histamine, NCS-1 overexpression led to higher exocytosis, as well as [Ca²⁺]_i elevation. Immunocytochemistry showed a similar distribution of NCS-1 and phosphatidylinositol 4-kinase  (PI4K). NCS-1 and PI4K coimmunoprecipitate, opening up the possibility that the two proteins directly interact. These results suggest that NCS-1 may regulate cellular activity through the modulation of the phosphatidylinositol signaling pathway.

Key words: neuronal calcium sensor; NCS-1; chromaffin; exocytosis; calcium; histamine; phosphatidylinositol; IP₃; Semliki Forest virus vector

---

Copyright © 2002 Society for Neuroscience  0270-6474/02/2272427-07$05.00/0

This article has been cited by other articles:

				A. A. Long, E. Kim, H.-T. Leung, E. Woodruff III, L. An, R. W. Doerge, W. L. Pak, and K. Broadie Presynaptic Calcium Channel Localization and Calcium-Dependent Synaptic Vesicle Exocytosis Regulated by the Fuseless Protein J. Neurosci., April 2, 2008; 28(14): 3668 - 3682. [Abstract] [Full Text] [PDF]

				C. C. Hernandez, O. Zaika, G. P. Tolstykh, and M. S. Shapiro Regulation of neural KCNQ channels: signalling pathways, structural motifs and functional implications J. Physiol., April 1, 2008; 586(7): 1811 - 1821. [Abstract] [Full Text] [PDF]

				S. Alesci, S. M. Perera, E. W. Lai, C. Kukura, M. Abu-Asab, M. Tsokos, J. C. Morris, and K. Pacak Adenoviral Gene Transfer in Bovine Adrenomedullary and Murine Pheochromocytoma Cells: Potential Clinical and Therapeutic Relevance Endocrinology, August 1, 2007; 148(8): 3900 - 3907. [Abstract] [Full Text] [PDF]

				R. C. E. Wykes, C. S. Bauer, S. U. Khan, J. L. Weiss, and E. P. Seward Differential Regulation of Endogenous N- and P/Q-Type Ca2+ Channel Inactivation by Ca2+/Calmodulin Impacts on Their Ability to Support Exocytosis in Chromaffin Cells J. Neurosci., May 9, 2007; 27(19): 5236 - 5248. [Abstract] [Full Text] [PDF]

				C. S. Bauer, R. J. Woolley, A. G. Teschemacher, and E. P. Seward Potentiation of Exocytosis by Phospholipase C-Coupled G-Protein-Coupled Receptors Requires the Priming Protein Munc13-1 J. Neurosci., January 3, 2007; 27(1): 212 - 219. [Abstract] [Full Text] [PDF]

				R. Thiagarajan, J. Wilhelm, T. Tewolde, Y. Li, M. M. Rich, and K. L. Engisch Enhancement of Asynchronous and Train-Evoked Exocytosis in Bovine Adrenal Chromaffin Cells Infected With a Replication Deficient Adenovirus J Neurophysiol, November 1, 2005; 94(5): 3278 - 3291. [Abstract] [Full Text] [PDF]

				N. Gamper, V. Reznikov, Y. Yamada, J. Yang, and M. S. Shapiro Phosphotidylinositol 4,5-Bisphosphate Signals Underlie Receptor-Specific Gq/11-Mediated Modulation of N-Type Ca2+ Channels J. Neurosci., December 1, 2004; 24(48): 10980 - 10992. [Abstract] [Full Text] [PDF]

				J. Andrade, H. Zhao, B. Titus, S. Timm Pearce, and M. Barroso The EF-Hand Ca2+-binding Protein p22 Plays a Role in Microtubule and Endoplasmic Reticulum Organization and Dynamics with Distinct Ca2+-binding Requirements Mol. Biol. Cell, February 1, 2004; 15(2): 481 - 496. [Abstract] [Full Text] [PDF]

				T. Strahl, B. Grafelmann, J. Dannenberg, J. Thorner, and O. Pongs Conservation of Regulatory Function in Calcium-binding Proteins: HUMAN FREQUENIN (NEURONAL CALCIUM SENSOR-1) ASSOCIATES PRODUCTIVELY WITH YEAST PHOSPHATIDYLINOSITOL 4-KINASE ISOFORM, Pik1 J. Biol. Chem., December 5, 2003; 278(49): 49589 - 49599. [Abstract] [Full Text] [PDF]

				D. W. O'Callaghan, B. Hasdemir, M. Leighton, and R. D. Burgoyne Residues within the myristoylation motif determine intracellular targeting of the neuronal Ca2+ sensor protein KChIP1 to post-ER transport vesicles and traffic of Kv4 K+ channels J. Cell Sci., December 1, 2003; 116(23): 4833 - 4845. [Abstract] [Full Text] [PDF]

				M. Rousset, T. Cens, S. Gavarini, A. Jeromin, and P. Charnet Down-regulation of Voltage-gated Ca2+ Channels by Neuronal Calcium Sensor-1 Is beta Subunit-specific J. Biol. Chem., February 21, 2003; 278(9): 7019 - 7026. [Abstract] [Full Text] [PDF]

				N. Kabbani, L. Negyessy, R. Lin, P. Goldman-Rakic, and R. Levenson Interaction with Neuronal Calcium Sensor NCS-1 Mediates Desensitization of the D2 Dopamine Receptor J. Neurosci., October 1, 2002; 22(19): 8476 - 8486. [Abstract] [Full Text] [PDF]

				S. Koizumi, P. Rosa, G. B. Willars, R. A. J. Challiss, E. Taverna, M. Francolini, M. D. Bootman, P. Lipp, K. Inoue, J. Roder, et al. Mechanisms Underlying the Neuronal Calcium Sensor-1-evoked Enhancement of Exocytosis in PC12 Cells J. Biol. Chem., August 9, 2002; 277(33): 30315 - 30324. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help