WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience MBF Stereo Investigator
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (20)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hu, J.-Y.
Right arrow Articles by Schacher, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hu, J.-Y.
Right arrow Articles by Schacher, S.

 Previous Article  |  Next Article 

The Journal of Neuroscience, April 1, 2002, 22(7):2669-2678

Target Interaction Regulates Distribution and Stability of Specific mRNAs

Jiang-Yuan Hu, Xu Meng, and Samuel Schacher

Center for Neurobiology and Behavior, Columbia University College of Physicians and Surgeons, and New York State Psychiatric Institute, New York, New York 10032

Several factors regulate export of mRNAs from neuronal cell bodies. Using in situ hybridization and RT-PCR, we examined how target interaction influences the distribution of mRNAs expressed in sensory neurons (SNs) of Aplysia maintained in cell culture. Interaction with a synaptic target has two effects on the distribution of mRNA encoding an SN-specific peptide, sensorin: the target affects the accumulation of sensorin mRNA at the axon hillock and the stability of sensorin mRNA exported to distal sites. Synapse formation with motor neuron L7 results in the accumulation of high levels of sensorin mRNA in the axon hillock of the SN and in SN neurites contacting L7. SNs cultured alone or in contact with motor neuron L11, with which no synapses form, show a more uniform distribution of sensorin mRNA in the cytoplasm of the SN cell body, with little expression in neurites. Contact with L7 or L11 had little or no effect on the distribution of two other mRNAs in the cytoplasm of SN cell bodies. Sensorin mRNA exported to SN neurites after 1 d in culture is more stable when the SN contacts L7 compared with SN neurites that contact L11. After removal of the SN cell body, the amounts of sensorin mRNA already exported to the neurites are greater when neurites contact L7 compared with neurites in contact with L11. The results indicate that target interaction and synapse formation regulate both the accumulations of specific mRNAs destined for export and their stability at distant sites.

Key words: synapse formation; synapse specificity; mRNA expression; mRNA export; mRNA stability; cell culture; Aplysia

Copyright © 2002 Society for Neuroscience  0270-6474/02/2272669-10$05.00/0


This article has been cited by other articles:


Home page
 J. Neurosci.Home page
J.-Y. Hu, Y. Chen, and S. Schacher
Multifunctional Role of Protein Kinase C in Regulating the Formation and Maturation of Specific Synapses
J. Neurosci., October 24, 2007; 27(43): 11712 - 11724.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J.-Y. Hu, Y. Chen, and S. Schacher
Protein Kinase C Regulates Local Synthesis and Secretion of a Neuropeptide Required for Activity-Dependent Long-Term Synaptic Plasticity
J. Neurosci., August 15, 2007; 27(33): 8927 - 8939.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. A. Marti, X. Li, S. Jockusch, Z. Li, B. Raveendra, S. Kalachikov, J. J. Russo, I. Morozova, S. V. Puthanveettil, J. Ju, et al.
Pyrene binary probes for unambiguous detection of mRNA using time-resolved fluorescence spectroscopy
Nucleic Acids Res., June 12, 2006; 34(10): 3161 - 3168.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. Liu, J.-Y. Hu, F. Wu, J. H. Schwartz, and S. Schacher
Two mRNA-Binding Proteins Regulate the Distribution of Syntaxin mRNA in Aplysia Sensory Neurons.
J. Neurosci., May 10, 2006; 26(19): 5204 - 5214.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J.-Y. Hu, F. Wu, and S. Schacher
Two Signaling Pathways Regulate the Expression and Secretion of a Neuropeptide Required for Long-Term Facilitation in Aplysia
J. Neurosci., January 18, 2006; 26(3): 1026 - 1035.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J.-Y. Hu, J. Goldman, F. Wu, and S. Schacher
Target-Dependent Release of a Presynaptic Neuropeptide Regulates the Formation and Maturation of Specific Synapses in Aplysia
J. Neurosci., November 3, 2004; 24(44): 9933 - 9943.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. Liu, J.-Y. Hu, S. Schacher, and J. H. Schwartz
The Two Regulatory Subunits of Aplysia cAMP-Dependent Protein Kinase Mediate Distinct Functions in Producing Synaptic Plasticity
J. Neurosci., March 10, 2004; 24(10): 2465 - 2474.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
J. Bi, X. Hu, H. H. Loh, and L.-N. Wei
Mouse {kappa}-Opioid Receptor mRNA Differential Transport in Neurons
Mol. Pharmacol., September 1, 2003; 64(3): 594 - 599.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J.-Y. Hu, X. Meng, and S. Schacher
Redistribution of Syntaxin mRNA in Neuronal Cell Bodies Regulates Protein Expression and Transport during Synapse Formation and Long-Term Synaptic Plasticity
J. Neurosci., March 1, 2003; 23(5): 1804 - 1815.
[Abstract] [Full Text] [PDF]


-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help
-
Copyright 2008 by Society for Neuroscience ONLINE ISSN: 1529-2401
-