QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, June 1, 2003, 23(11):4509-4518

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (30) Citing Articles via Google Scholar Google Scholar Articles by Devaux, J. Articles by Scherer, S. S. Search for Related Content PubMed PubMed Citation Articles by Devaux, J. Articles by Scherer, S. S. Pubmed/NCBI databases Gene GEO Profiles HomoloGene Nucleotide Protein UniGene Compound via MeSH Substance via MeSH Hazardous Substances DB 4-AMINOPYRIDINE

 Previous Article  |  Next Article 

Kv3.1b Is a Novel Component of CNS Nodes

Jérôme Devaux,^1,6 Gisèle Alcaraz,² Judith Grinspan,³ Vann Bennett,⁴ Rolf Joho,⁵ Marcel Crest,⁶ and Steven S. Scherer¹

¹ Department of Neurology, The University of Pennsylvania Medical Center, Philadelphia, Pennsylvania 19104-6077, ² Institut National de la Santé et de la Recherche Médicale Unité 464, Institut Jean Roche, Faculté de Médecine Nord, Université de la Méditerranée, 13916 Marseille Cedex 20, France, ³ Division of Neurology Research, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 19104-6077, ⁴ Howard Hughes Medical Institute and Departments of Biochemistry and Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, ⁵ Center for Basic Neuroscience, The University of Texas Southwestern Medical Center, Dallas, Texas 75390-9111, and ⁶ Laboratoire Intégration des Informations Sensorielles, Centre National de la Recherche Scientifique, 13402 Marseille Cedex 20, France

We herein demonstrate that Kv3.1b subunits are present at nodes of Ranvier in the CNS of both rats and mice. Kv3.1b colocalizes with voltage-gated Na⁺ channels in a subset of nodes in the spinal cord, particularly those of large myelinated axons. Kv3.1b is abundantly expressed in the gray matter of the spinal cord, but does not colocalize with Na⁺ channels in initial segments. In the PNS, few nodes are Kv3.1b-positive. During the development of the CNS, Kv3.1b clustering at nodes occurs later than that of Na⁺ channels, but precedes the juxtaparanodal clustering of Kv1.2. Moreover, in myelin-deficient rats, which have severe CNS dysmyelination, node-like clusters of Kv3.1b and Na⁺ channels are observed even in regions devoid of oligodendrocytes. Ankyrin G coimmunoprecipitates Kv3.1b in vivo, indicating that these two proteins may interact in the CNS at nodes. 4-Aminopyridine, a K⁺ channel blocker, broadened the compound action potential recorded from adult rat optic nerve and spinal cord, but not from the sciatic nerve. These effects were also observed in Kv3.1-deficient mice. In conclusion, Kv3.1b is the first K⁺ channel subunit to be identified in CNS nodes; but Kv3.1b does not account for the effects of 4-aminopyridine on central myelinated tracts.

Key words: Shaw; potassium channels; oligodendrocyte; Schwann cells; myelin; multiple sclerosis

---

Received Aug. 22, 2002; revised Feb. 21, 2003; accepted Mar. 12, 2003.

This article has been cited by other articles:

				M. Xu, R. Cao, R. Xiao, M. X. Zhu, and C. Gu The Axon Dendrite Targeting of Kv3 (Shaw) Channels Is Determined by a Targeting Motif That Associates with the T1 Domain and Ankyrin G J. Neurosci., December 19, 2007; 27(51): 14158 - 14170. [Abstract] [Full Text] [PDF]

				S. Tiwari-Woodruff, L. Beltran-Parrazal, A. Charles, T. Keck, T. Vu, and J. Bronstein K+ channel KV3.1 associates with OSP/claudin-11 and regulates oligodendrocyte development Am J Physiol Cell Physiol, October 1, 2006; 291(4): C687 - C698. [Abstract] [Full Text] [PDF]

				K. A. Kleopa, L. B. Elman, B. Lang, A. Vincent, and S. S. Scherer Neuromyotonia and limbic encephalitis sera target mature Shaker-type K+ channels: subunit specificity correlates with clinical manifestations Brain, June 1, 2006; 129(6): 1570 - 1584. [Abstract] [Full Text] [PDF]

				M. C. Inda, J. DeFelipe, and A. Munoz Voltage-gated ion channels in the axon initial segment of human cortical pyramidal cells and their relationship with chandelier cells PNAS, February 21, 2006; 103(8): 2920 - 2925. [Abstract] [Full Text] [PDF]

				J. J. Devaux and S. S. Scherer Altered Ion Channels in an Animal Model of Charcot-Marie-Tooth Disease Type IA J. Neurosci., February 9, 2005; 25(6): 1470 - 1480. [Abstract] [Full Text] [PDF]

				Y. Yang, S. Lacas-Gervais, D. K. Morest, M. Solimena, and M. N. Rasband {beta}IV Spectrins Are Essential for Membrane Stability and the Molecular Organization of Nodes of Ranvier J. Neurosci., August 18, 2004; 24(33): 7230 - 7240. [Abstract] [Full Text] [PDF]

				J. J. Devaux, K. A. Kleopa, E. C. Cooper, and S. S. Scherer KCNQ2 Is a Nodal K+ Channel J. Neurosci., February 4, 2004; 24(5): 1236 - 1244. [Abstract] [Full Text] [PDF]

				S. Poliak, D. Salomon, H. Elhanany, H. Sabanay, B. Kiernan, L. Pevny, C. L. Stewart, X. Xu, S.-Y. Chiu, P. Shrager, et al. Juxtaparanodal clustering of Shaker-like K+ channels in myelinated axons depends on Caspr2 and TAG-1 J. Cell Biol., September 15, 2003; 162(6): 1149 - 1160. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help