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The Journal of Neuroscience, June 15, 2003, 23(12):4942-4950

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Chloride Influx Aggravates Ca²⁺-Dependent AMPA Receptor-Mediated Motoneuron Death

P. Van Damme,¹ G. Callewaert,² J. Eggermont,² W. Robberecht,¹ and L. Van Den Bosch¹

Departments of ¹Neurobiology and ²Physiology, Catholic University Leuven, Campus Gasthuisberg, B-3000 Leuven, Belgium

AMPA receptor-mediated excitotoxicity has been implicated in the pathogenesis of stroke, neurotrauma, epilepsy, and many neurodegenerative diseases such as motoneuron disease. We studied the role of Cl^- in AMPA receptor-mediated Ca²⁺-dependent excitotoxicity in cultured rat spinal motoneurons. Using the gramicidin perforated patch-clamp technique, the intracellular Cl^- concentration could be calculated from the reversal potential of the GABA-induced current. The membrane depolarization caused by AMPA receptor stimulation resulted in Cl^- influx through 5-nitro-2(3-phenylpropyl-amino) benzoic acid- and niflumic acid-sensitive Cl^- channels. Cl^- influx during AMPA receptor stimulation aggravated excitotoxic motoneuron death by two mechanisms: an increase of AMPA receptor conductance and an elevation of the Ca²⁺ driving force through a partial repolarization. The Cl^- influx during AMPA receptor stimulation was enhanced by coadministration of GABA. This resulted in an increased Ca²⁺ influx and an enhanced cell death, suggesting that concomitant GABAergic stimulation may aggravate excitotoxic motoneuron death.

Key words: AMPA receptor; calcium; motoneuron; chloride; excitotoxicity; amyotrophic lateral sclerosis

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Received Oct. 16, 2002; revised Apr. 8, 2003; accepted Apr. 8, 2003.

This article has been cited by other articles:

				J. N. Chabwine, K. Talavera, L. Verbert, J. Eggermont, J.-M. Vanderwinden, H. De Smedt, L. Van Den Bosch, W. Robberecht, and G. Callewaert Differential contribution of the Na+-K+-2Cl- cotransporter NKCC1 to chloride handling in rat embryonic dorsal root ganglion neurons and motor neurons FASEB J, April 1, 2009; 23(4): 1168 - 1176. [Abstract] [Full Text] [PDF]

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