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The Journal of Neuroscience, July 16, 2003, 23(15):6232-6237

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Tenascin-R as a Repellent Guidance Molecule for Developing Optic Axons in Zebrafish

Catherina G. Becker, Jörn Schweitzer, Julia Feldner, Thomas Becker, and Melitta Schachner

Zentrum für Molekulare Neurobiologie Hamburg, Universität Hamburg, D-20246 Hamburg, Germany

To investigate the role of tenascin-R in nervous system development, we studied axon pathfinding in the developing optic system of zebrafish. Zebrafish tenascin-R has the same domain structure as tenascin-R in amniotes. Amino acid sequence identity with human tenascin-R is 60%. In 3-d-old larvae, tenascin-R mRNA is expressed in scattered cells throughout the periventricular cell layer of the diencephalon and tectum. Tenascin-R immunoreactivity is not detectable in the optic nerve, optic tract, or tectal optic neuropil but immediately borders the optic tract caudally. Reducing expression of tenascin-R in 3-d-old larvae in vivo by injecting morpholinos into fertilized eggs led to excessive branching of the optic tract in 86% of all injected larvae compared with 20–37% in controls. Branches were almost exclusively caudal, where tenascin-R immunoreactivity normally borders the optic tract, suggesting a role for tenascin-R in guiding optic axons in the ventral diencephalon by a contact-repellent mechanism.

Key words: extracellular matrix; chondroitin sulfate proteoglycans; semaphorin; slit; axon guidance; Danio rerio

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Received Mar. 31, 2003; revised May. 13, 2003; accepted May. 16, 2003.

This article has been cited by other articles:

				I. Apostolova, A. Irintchev, and M. Schachner Tenascin-R restricts posttraumatic remodeling of motoneuron innervation and functional recovery after spinal cord injury in adult mice. J. Neurosci., July 26, 2006; 26(30): 7849 - 7859. [Abstract] [Full Text] [PDF]

				T. Xiao, T. Roeser, W. Staub, and H. Baier A GFP-based genetic screen reveals mutations that disrupt the architecture of the zebrafish retinotectal projection Development, July 1, 2005; 132(13): 2955 - 2967. [Abstract] [Full Text] [PDF]

				F. de Winter, Q. Cui, N. Symons, J. Verhaagen, and A. R. Harvey Expression of Class-3 Semaphorins and Their Receptors in the Neonatal and Adult Rat Retina Invest. Ophthalmol. Vis. Sci., December 1, 2004; 45(12): 4554 - 4562. [Abstract] [Full Text] [PDF]

				C. G. Becker, B. C. Lieberoth, F. Morellini, J. Feldner, T. Becker, and M. Schachner L1.1 Is Involved in Spinal Cord Regeneration in Adult Zebrafish J. Neurosci., September 8, 2004; 24(36): 7837 - 7842. [Abstract] [Full Text] [PDF]

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