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The Journal of Neuroscience, October 15, 2003, 23(28):9328-9339
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Cellular/Molecular
Correlation between Olfactory Receptor Cell Type and Function in the Channel Catfish
Anne Hansen,1
Shane H. Rolen,2
Karl Anderson,1
Yasuhiro Morita,1,3
John Caprio,2 and
Thomas E. Finger1
1Department of Cellular and Structural Biology, University of Colorado Health Sciences Center, Denver, Colorado 80262, 2Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana 70830, and 3Department of Anatomy and Physiology, Kagawa Prefecture College, Kagawa 761-0123, Japan
The olfactory epithelium of fish contains three intermingled types of olfactory receptor neurons (ORNs): ciliated, microvillous, and crypt. The present experiments were undertaken to test whether the different types of ORNs respond to different classes of odorants via different families of receptor molecules and G-proteins corresponding to the morphology of the ORN. In catfish, ciliated ORNs express OR-type receptors and G olf. Microvillous ORNs are heterogeneous, with many expressing Gq/11, whereas crypt ORNs express Go. Retrograde tracing experiments show that ciliated ORNs project predominantly to regions of the olfactory bulb (OB) that respond to bile salts (medial) and amino acids (ventral) (Nikonov and Caprio, 2001). In contrast, microvillous ORNs project almost entirely to the dorsal surface of the OB, where responses to nucleotides (posterior OB) and amino acids (anterior OB) predominate. These anatomical findings are consistent with our pharmacological results showing that forskolin (which interferes with Golf/cAMP signaling) blocks responses to bile salts and markedly reduces responses to amino acids. Conversely, U-73122 and U-73343 (which interfere with Gq/11/phospholipase C signaling) diminish amino acid responses but leave bile salt and nucleotide responses essentially unchanged. In summary, our results indicate that bile salt odorants are detected predominantly by ciliated ORNs relying on the Golf/cAMP transduction cascade. Nucleotides are detected by microvillous ORNs using neither Golf/cAMP nor Gq/11/PLC cascades. Finally, amino acid odorants activate both ciliated and microvillous ORNs but via different transduction pathways in the two types of cells.
Key words: olfactory; receptor; G-protein; amino acid; bile salt; transduction
Received June 9, 2003;
revised August 23, 2003;
accepted August 24, 2003.
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