QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, October 22, 2003, 23(29):9547-9556

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (18) Citing Articles via Google Scholar Google Scholar Articles by Sun, T. Articles by Stiles, C. D. Search for Related Content PubMed PubMed Citation Articles by Sun, T. Articles by Stiles, C. D.

 Previous Article  |  Next Article 

Cellular/Molecular
Cross-Repressive Interaction of the Olig2 and Nkx2.2 Transcription Factors in Developing Neural Tube Associated with Formation of a Specific Physical Complex

Tao Sun,^1 * Hualing Dong,^2 * Lizi Wu,³ Michael Kane,² David H. Rowitch,¹ and Charles D. Stiles²

Departments of ¹Pediatric Oncology, ²Cancer Biology, and ³Adult Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115

In developing neural tube, the basic helix-loop-helix (bHLH) transcription factor Olig2 interacts with the homeodomain transcription factor Nkx2.2 at two distinct stages. During neuronogenesis, a cross-repressive interaction appears to establish the precise boundary between the p3 and pMN domains. At later times, a cooperative interaction is noted because Nkx2.2 promotes maturation of oligodendrocyte progenitor cells specified by expression of Olig2. We show here that the Olig2 protein can form a physical complex with Nkx2.2 protein in mammalian cells and yeast two-hybrid trap assay. This interaction is specific because Olig2 does not bind to a biologically irrelevant homeodomain transcription factor (Nkx6.1), and Nkx2.2 does not interact with a biologically irrelevant bHLH protein (NeuroD). Deletion mapping analysis suggests that formation of an Olig2-Nkx2.2 physical complex is insufficient for the induction of oligodendrocyte progenitors in developing spine; however, the protein-protein interaction observed might be important for the cross-repressive interaction between Olig2 and Nkx2.2 that helps to establish the pMN-p3 boundary in the developing spinal cord.

Key words: Nkx2.2; Olig2; homeodomain; bHLH; neural tube; neuronal subtype specification; oligodendrocyte; interneuron

---

Received April 28, 2003; revised August 26, 2003; accepted August 28, 2003.

This article has been cited by other articles:

				L. Lyck, I. Dalmau, J. Chemnitz, B. Finsen, and H. D. Schroder Immunohistochemical Markers for Quantitative Studies of Neurons and Glia in Human Neocortex J. Histochem. Cytochem., March 1, 2008; 56(3): 201 - 221. [Abstract] [Full Text] [PDF]

				N. Kessaris, N. Pringle, and W. D Richardson Specification of CNS glia from neural stem cells in the embryonic neuroepithelium Phil Trans R Soc B, January 12, 2008; 363(1489): 71 - 85. [Abstract] [Full Text] [PDF]

				H. Li, Y. Lu, H. K. Smith, and W. D. Richardson Olig1 and Sox10 Interact Synergistically to Drive Myelin Basic Protein Transcription in Oligodendrocytes J. Neurosci., December 26, 2007; 27(52): 14375 - 14382. [Abstract] [Full Text] [PDF]

				F. Guillemot Spatial and temporal specification of neural fates by transcription factor codes Development, November 1, 2007; 134(21): 3771 - 3780. [Abstract] [Full Text] [PDF]

				S. Gokhan, M. Marin-Husstege, S. Y. Yung, D. Fontanez, P. Casaccia-Bonnefil, and M. F. Mehler Combinatorial Profiles of Oligodendrocyte-Selective Classes of Transcriptional Regulators Differentially Modulate Myelin Basic Protein Gene Expression J. Neurosci., September 7, 2005; 25(36): 8311 - 8321. [Abstract] [Full Text] [PDF]

				Q. Wei, W. K. Miskimins, and R. Miskimins Stage-specific Expression of Myelin Basic Protein in Oligodendrocytes Involves Nkx2.2-mediated Repression That Is Relieved by the Sp1 Transcription Factor J. Biol. Chem., April 22, 2005; 280(16): 16284 - 16294. [Abstract] [Full Text] [PDF]

				S.-K. Lee, B. Lee, E. C. Ruiz, and S. L. Pfaff Olig2 and Ngn2 function in opposition to modulate gene expression in motor neuron progenitor cells Genes & Dev., January 15, 2005; 19(2): 282 - 294. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help