QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, October 22, 2003, 23(29):9664-9674

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (34) Citing Articles via Google Scholar Google Scholar Articles by Bacci, A. Articles by Prince, D. A. Search for Related Content PubMed PubMed Citation Articles by Bacci, A. Articles by Prince, D. A.

 Previous Article  |  Next Article 

Cellular/Molecular
Major Differences in Inhibitory Synaptic Transmission onto Two Neocortical Interneuron Subclasses

Alberto Bacci,¹ Uwe Rudolph,² John R. Huguenard,¹ and David A. Prince¹

¹Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, California 94305, and ²Institute of Pharmacology and Toxicology, University of Zürich, CH-8057 Zürich, Switzerland

Locally projecting GABAergic interneurons are the major providers of inhibition in the neocortex and play a crucial role in several brain functions. Neocortical interneurons are connected via electrical and chemical synapses that may be crucial in modulating complex network oscillations. We investigated the properties of spontaneous and evoked IPSCs in two morphologically and physiologically identified interneuron subtypes, the fast-spiking (FS) and low threshold-spiking (LTS) cells in layer V of rodent sensorimotor cortex. We found that IPSCs recorded in FS cells were several orders of magnitude more frequent, larger in amplitude, and had faster kinetics than IPSCs recorded in LTS cells. GABA_A receptor - and -subunit selective modulators, zolpidem and loreclezole, had different effects on IPSCs in FS and LTS interneurons, suggesting differential expression of GABA_A receptor subunit subtypes. These pharmacological data indicated that the 1 subunit subtype is poorly expressed by LTS cells but makes a large contribution to GABA_A receptors on FS cells. This was confirmed by experiments performed in genetically modified mice in which the 1 subunit had been made insensitive to benzodiazepine-like agonists. These results suggest that differences in IPSC waveform are likely attributable to distinctive expression of GABA_A receptor subunits in FS and LTS cells. The particular properties of GABAergic input on different interneuronal subtypes might have important consequences for generation and pacing of cortical rhythms underlying several brain functions. Moreover, selective pharmacological manipulation of distinct inhibitory circuits might allow regulation of pyramidal cell activities under specific physiological and pathophysiological conditions.

Key words: interneurons; neocortex; synaptic transmission; GABA; GABA_A receptors; inhibition

---

Received June 12, 2003; revised September 2, 2003; accepted September 3, 2003.

This article has been cited by other articles:

				G. Gonzalez-Burgos and D. A. Lewis GABA Neurons and the Mechanisms of Network Oscillations: Implications for Understanding Cortical Dysfunction in Schizophrenia Schizophr Bull, September 1, 2008; 34(5): 944 - 961. [Abstract] [Full Text] [PDF]

				V. C. Kotak, A. E. Takesian, and D. H. Sanes Hearing Loss Prevents the Maturation of GABAergic Transmission in the Auditory Cortex Cereb Cortex, September 1, 2008; 18(9): 2098 - 2108. [Abstract] [Full Text] [PDF]

				E. I. Krook-Magnuson, P. Li, S. M. Paluszkiewicz, and M. M. Huntsman Tonically Active Inhibition Selectively Controls Feedforward Circuits in Mouse Barrel Cortex J Neurophysiol, August 1, 2008; 100(2): 932 - 944. [Abstract] [Full Text] [PDF]

				E. Krook-Magnuson and M. M. Huntsman The transience of interneuron circuit diversity just "sped" up PNAS, October 23, 2007; 104(43): 16723 - 16724. [Full Text] [PDF]

				J.-t. Lu, C.-y. Li, J.-P. Zhao, M.-m. Poo, and X.-h. Zhang Spike-Timing-Dependent Plasticity of Neocortical Excitatory Synapses on Inhibitory Interneurons Depends on Target Cell Type J. Neurosci., September 5, 2007; 27(36): 9711 - 9720. [Abstract] [Full Text] [PDF]

				K. Morita, M. Okada, and K. Aihara Selectivity and Stability via Dendritic Nonlinearity. Neural Comput., July 1, 2007; 19(7): 1798 - 1853. [Abstract] [Full Text] [PDF]

				B. Halabisky, F. Shen, J. R. Huguenard, and D. A. Prince Electrophysiological Classification of Somatostatin-Positive Interneurons in Mouse Sensorimotor Cortex J Neurophysiol, August 1, 2006; 96(2): 834 - 845. [Abstract] [Full Text] [PDF]

				G. A. Prenosil, E. M. Schneider Gasser, U. Rudolph, R. Keist, J.-M. Fritschy, and K. E. Vogt Specific Subtypes of GABAA Receptors Mediate Phasic and Tonic Forms of Inhibition in Hippocampal Pyramidal Neurons J Neurophysiol, August 1, 2006; 96(2): 846 - 857. [Abstract] [Full Text] [PDF]

				J. R. Gibson, A. F. Bartley, and K. M. Huber Role for the Subthreshold Currents ILeak and IH in the Homeostatic Control of Excitability in Neocortical Somatostatin-Positive Inhibitory Neurons J Neurophysiol, July 1, 2006; 96(1): 420 - 432. [Abstract] [Full Text] [PDF]

				X. Jin, D. A. Prince, and J. R. Huguenard Enhanced excitatory synaptic connectivity in layer v pyramidal neurons of chronically injured epileptogenic neocortex in rats. J. Neurosci., May 3, 2006; 26(18): 4891 - 4900. [Abstract] [Full Text] [PDF]

				V. S. Sohal, S. Pangratz-Fuehrer, U. Rudolph, and J. R. Huguenard Intrinsic and synaptic dynamics interact to generate emergent patterns of rhythmic bursting in thalamocortical neurons. J. Neurosci., April 19, 2006; 26(16): 4247 - 4255. [Abstract] [Full Text] [PDF]

				M. M. Huntsman and J. R. Huguenard Fast IPSCs in rat thalamic reticular nucleus require the GABAA receptor {beta}1 subunit J. Physiol., April 15, 2006; 572(2): 459 - 475. [Abstract] [Full Text] [PDF]

				V. S. Sohal and J. R. Huguenard Inhibitory coupling specifically generates emergent gamma oscillations in diverse cell types PNAS, December 20, 2005; 102(51): 18638 - 18643. [Abstract] [Full Text] [PDF]

				L.-R. Shao and F. E. Dudek Changes in mIPSCs and sIPSCs After Kainate Treatment: Evidence for Loss of Inhibitory Input to Dentate Granule Cells and Possible Compensatory Responses J Neurophysiol, August 1, 2005; 94(2): 952 - 960. [Abstract] [Full Text] [PDF]

				R. D. Traub, D. Contreras, M. O. Cunningham, H. Murray, F. E. N. LeBeau, A. Roopun, A. Bibbig, W. B. Wilent, M. J. Higley, and M. A. Whittington Single-Column Thalamocortical Network Model Exhibiting Gamma Oscillations, Sleep Spindles, and Epileptogenic Bursts J Neurophysiol, April 1, 2005; 93(4): 2194 - 2232. [Abstract] [Full Text] [PDF]

				M. Blatow, A. Caputi, and H. Monyer Molecular diversity of neocortical GABAergic interneurones J. Physiol., January 1, 2005; 562(1): 99 - 105. [Abstract] [Full Text] [PDF]

				N. Kopell and B. Ermentrout Chemical and electrical synapses perform complementary roles in the synchronization of interneuronal networks PNAS, October 26, 2004; 101(43): 15482 - 15487. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help