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The Journal of Neuroscience, February 1, 2003, 23(3):837
Involvement of Actin Polymerization in Vesicle
Recruitment at the Calyx of Held Synapse
Takeshi
Sakaba and
Erwin
Neher
Department of Membrane Biophysics, Max-Planck-Institute for
Biophysical Chemistry, Göttingen, D-37077, Germany
Depletion and replenishment of pools of synaptic vesicles are
important determinants of short-term synaptic plasticity, but the
underlying molecular mechanisms are not yet clear. As a first step
toward understanding the process of vesicle recruitment, we have
applied various specific agents directly to the presynaptic terminal of
the calyx of Held synapse. Here we show that the nonhydrolyzable ATP
analog ATP- S retards the recovery from vesicle pool depletion, as
does latrunculin A. Phalloidin has no effects on recovery, suggesting
that dynamic actin reorganization is not necessary. Unexpectedly,
neither N-ethylmaleimide nor staurosporine affected the
recovery, calling into question the role of
N-ethylmaleimide-sensitive factor and protein
kinases. The results suggest that intact actin polymerization is
involved in vesicle recruitment.
Key words:
synaptic transmission; synaptic depression; synaptic plasticity; vesicle pool; vesicle recruitment; actin
Copyright © 2003 Society for Neuroscience 0270-6474/03/233837-10$05.00/0
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