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The Journal of Neuroscience, April 15, 2003, 23(8):3251
Activity-Dependent Trafficking and Dynamic Localization of
Zipcode Binding Protein 1 and  -Actin mRNA in Dendrites and Spines of
Hippocampal Neurons
Dhanrajan M.
Tiruchinapalli1,
Yuri
Oleynikov2,
Sofija
Keli 1,
Shailesh M.
Shenoy2,
Adam
Hartley1,
Patric K.
Stanton1,
Robert H.
Singer2, and
Gary J.
Bassell1
1 Department of Neuroscience, Rose F. Kennedy Center
for Research in Mental Retardation and Human Development, and
2 Department of Anatomy and Structural Biology, Albert
Einstein College of Medicine, Bronx, New York 10461
RNA binding proteins may be important mediators of the
activity-dependent transport of mRNAs to dendritic spines of activated synapses. We used fluorescence microscopy and digital imaging techniques applied to both fixed and live cultured hippocampal neurons
to visualize the localization of the mRNA binding protein, zipcode
binding protein 1 (ZBP1), and its dynamic movements in response to
KCl-induced depolarization at high spatial and temporal resolution.
With the use of immunofluorescence, image deconvolution, and
three-dimensional reconstruction, ZBP1 was localized in the form of
granules that were distributed in dendrites, spines, and subsynaptic
sites. KCl depolarization increased the dendritic localization of ZBP1
that was not attributed to an increase in ZBP1 expression. Live cell
imaging of single cells before and after perfusion of KCl revealed the
rapid and directed efflux of ZBP1 granules from the cell body into
dendrites in a proximo-distal gradient. High-speed imaging of enhanced
green fluorescence protein-ZBP1 granules revealed rapid anterograde and
retrograde movements in dendrites as well as dynamic movements in
dendritic spines. A population of ZBP1 granules colocalized with
 -actin mRNA, and their spatial association in dendrites was
increased by KCl depolarization. The NMDA receptor antagonist AP-5
impaired the dendritic localization of ZBP1 and -actin mRNA and
inhibited the KCl-induced transport of ZBP1. The activity-dependent
trafficking of ZBP1 and its dynamic movements within dendritic spines
provide new evidence to implicate RNA binding proteins as regulators of
mRNA transport to activated synapses in response to synaptic activity.
Key words:
mRNA localization; mRNA binding protein; zipcode
binding protein; -actin mRNA; dendritic spines; NMDA receptors
Copyright © 2003 Society for Neuroscience 0270-6474/03/2383251-11$05.00/0
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