WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience The New Axio Examiner
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via ISI Web of Science (119)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kamme, F.
Right arrow Articles by Erlander, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kamme, F.
Right arrow Articles by Erlander, M.

 Previous Article  |  Next Article 

The Journal of Neuroscience, May 1, 2003, 23(9):3607

Single-Cell Microarray Analysis in Hippocampus CA1: Demonstration and Validation of Cellular Heterogeneity

Fredrik Kamme1, Ranelle Salunga2, Jingxue Yu1, Da-Thao Tran1, Jessica Zhu1, Lin Luo1, Anton Bittner1, Hong-Qing Guo1, Nancy Miller3, Jackson Wan1, and Mark Erlander2

1 Johnson & Johnson Pharmaceutical Research and Development LLC, San Diego, California 92121, 2 Arcturus Applied Genomics, Carlsbad, California 92008, and 3 OmniViz, Maynard, Massachusetts 01754

Laser capture microdissection in combination with microarrays allows for the expression analysis of thousands of genes in selected cells. Here we describe single-cell gene expression profiling of CA1 neurons in the rat hippocampus using a combination of laser capture, T7 RNA amplification, and cDNA microarray analysis. Subsequent cluster analysis of the microarray data identified two different cell types: pyramidal neurons and an interneuron. Cluster analysis also revealed differences among the pyramidal neurons, indicating that even a single cell type in vivo is not a homogeneous population of cells at the gene expression level. Microarray data were confirmed by quantitative RT-PCR and in situ hybridization. We also report on the reproducibility and sensitivity of this combination of methods. Single-cell gene expression profiling offers a powerful tool to tackle the complexity of the mammalian brain.

Key words: laser capture microdissection; gene expression profiling; RNA amplification; single cell; microarray; cell type definition

Copyright © 2003 Society for Neuroscience  0270-6474/03/2393607-09$05.00/0




-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help
-
Copyright 2008 by Society for Neuroscience ONLINE ISSN: 1529-2401
-