WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience MBF Stereo Investigator
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
The Journal of Neuroscience, March 31, 2004, 24(13):3251-3259; doi:10.1523/JNEUROSCI.5173-03.2004

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Figures
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (48)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Overstreet, L. S.
Right arrow Articles by Rubinstein, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Overstreet, L. S.
Right arrow Articles by Rubinstein, M.

 Previous Article  |  Next Article 

Development/Plasticity/Repair
A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus

Linda S. Overstreet,1 Shane T. Hentges,1 Viviana F. Bumaschny,4 Flavio S. J. de Souza,4 James L. Smart,1 Andrea M. Santangelo,4 Malcolm J. Low,1,2,3 Gary L. Westbrook,1 and Marcelo Rubinstein3,4,5

1Vollum Institute, 2Department of Behavioral Neuroscience, and 3The Center for Study of Weight Regulation and Associated Disorders, Oregon Health and Science University, Portland, Oregon 97239, 4Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas, y Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina, and 5Centro de Estudios Científicos, Casilla 1469, Valdivia, Chile

Neurogenesis in the dentate gyrus continues into adulthood, yet little is known about the function of newly born neurons or how they integrate into an existing network of mature neurons. We made transgenic mice that selectively and transiently express enhanced green fluorescent protein (EGFP) in newly born granule cells of the dentate gyrus under the transcriptional control of proopiomelanocortin (POMC) genomic sequences. Analysis of transgenic pedigrees with truncation or deletion mutations indicated that EGFP expression in the dentate gyrus required cryptic POMC promoter regions dispensable for arcuate hypothalamic or pituitary expression. Unlike arcuate neurons, dentate granule cells did not express the endogenous POMC gene. EGFP-positive neurons had immature properties, including short spineless dendrites and small action potentials. Colocalization with bromodeoxyuridine indicated that EGFP-labeled granule cells were ~2 weeks postmitotic. EGFP-labeled cells expressed markers for immature granule cells but not the glial marker GFAP. The number of EGFP-labeled neurons declined with age and increased with exercise, paralleling neurogenesis. Our results indicate that POMC-EGFP marks immature granule cells and that adult-generated granule cells integrate quite slowly into the hippocampal circuitry.

Key words: neurogenesis; neuronal progenitor; dentate granule cell; green fluorescent protein; transgenic mice; proopiomelanocortin; BrdU; PSA-nCAM

Received Nov 23, 2003; revised February 17, 2004; accepted February 18, 2004.




This article has been cited by other articles:


Home page
 J. Physiol.Home page
S. Vicini
The role of GABA and glutamate on adult neurogenesis
J. Physiol., August 15, 2008; 586(16): 3737 - 3738.
[Full Text] [PDF]

Home page
 J. Physiol.Home page
S. Ge, K. A. Sailor, G.-l. Ming, and H. Song
Synaptic integration and plasticity of new neurons in the adult hippocampus
J. Physiol., August 15, 2008; 586(16): 3759 - 3765.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
S. Markwardt and L. Overstreet-Wadiche
GABAergic signalling to adult-generated neurons
J. Physiol., August 15, 2008; 586(16): 3745 - 3749.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
X. Gao, P. Arlotta, J. D. Macklis, and J. Chen
Conditional Knock-Out of -Catenin in Postnatal-Born Dentate Gyrus Granule Neurons Results in Dendritic Malformation
J. Neurosci., December 26, 2007; 27(52): 14317 - 14325.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. R. Regan, Y. H. Huang, Y. S. Kim, M. I. Dykes-Hoberg, L. Jin, A. M. Watkins, D. E. Bergles, and J. D. Rothstein
Variations in Promoter Activity Reveal a Differential Expression and Physiology of Glutamate Transporters by Glia in the Developing and Mature CNS
J. Neurosci., June 20, 2007; 27(25): 6607 - 6619.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
L. Verret, J. L. Jankowsky, G. M. Xu, D. R. Borchelt, and C. Rampon
Alzheimer's-Type Amyloidosis in Transgenic Mice Impairs Survival of Newborn Neurons Derived from Adult Hippocampal Neurogenesis
J. Neurosci., June 20, 2007; 27(25): 6771 - 6780.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
T. L. Walker, T. Yasuda, D. J. Adams, and P. F. Bartlett
The Doublecortin-Expressing Population in the Developing and Adult Brain Contains Multipotential Precursors in Addition to Neuronal-Lineage Cells
J. Neurosci., April 4, 2007; 27(14): 3734 - 3742.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
L. S. Overstreet-Wadiche, D. A. Bromberg, A. L. Bensen, and G. L. Westbrook
Seizures Accelerate Functional Integration of Adult-Generated Granule Cells
J. Neurosci., April 12, 2006; 26(15): 4095 - 4103.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
L. S. Overstreet-Wadiche, A. L. Bensen, and G. L. Westbrook
Delayed development of adult-generated granule cells in dentate gyrus.
J. Neurosci., February 22, 2006; 26(8): 2326 - 2334.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
C. Zhao, E. M. Teng, R. G. Summers Jr, G.-l. Ming, and F. H. Gage
Distinct Morphological Stages of Dentate Granule Neuron Maturation in the Adult Mouse Hippocampus
J. Neurosci., January 4, 2006; 26(1): 3 - 11.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
L. O. Wadiche, D. A. Bromberg, A. L. Bensen, and G. L. Westbrook
GABAergic Signaling to Newborn Neurons in Dentate Gyrus
J Neurophysiol, December 1, 2005; 94(6): 4528 - 4532.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
H. Song, G. Kempermann, L. O. Wadiche, C. Zhao, A. F. Schinder, and J. Bischofberger
New Neurons in the Adult Mammalian Brain: Synaptogenesis and Functional Integration
J. Neurosci., November 9, 2005; 25(45): 10366 - 10368.
[Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
F. S. J. de Souza, A. M. Santangelo, V. Bumaschny, M. E. Avale, J. L. Smart, M. J. Low, and M. Rubinstein
Identification of Neuronal Enhancers of the Proopiomelanocortin Gene by Transgenic Mouse Analysis and Phylogenetic Footprinting
Mol. Cell. Biol., April 15, 2005; 25(8): 3076 - 3086.
[Abstract] [Full Text] [PDF]


-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help
-
Copyright 2008 by Society for Neuroscience ONLINE ISSN: 1529-2401
-