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The Journal of Neuroscience, January 14, 2004, 24(2):420-433; doi:10.1523/JNEUROSCI.4452-03.2004
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Cellular/Molecular
Competition between Phasic and Asynchronous Release for Recovered Synaptic Vesicles at Developing Hippocampal Autaptic Synapses
Yo Otsu,1,2 Vahid Shahrezaei,4,5 Bo Li,1,2 Lynn A. Raymond,1,2,3 Kerry R. Delaney,5 andTimothy H. Murphy1,2,3 1Kinsmen Laboratory and Brain Research Centre and Departments of 2Psychiatry and 3Physiology, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3, and Departments of 4Physics and 5Biological Sciences, Simon Fraser University, Burnaby, British Columbia, Canada V5A 1S6
Developing hippocampal neurons in microisland culture undergo rapid and extensive transmitter release-dependent depression of evoked (phasic) excitatory synaptic activity in response to 1 sec trains of 20 Hz stimulation. Although evoked phasic release was attenuated by repeated stimuli, asynchronous (miniature like) release continued at a high rate equivalent to
2.8 readily releasable pools (RRPs) of quanta/sec. Asynchronous release reflected the recovery and immediate release of quanta because it was resistant to sucrose-induced depletion of the RRP. Asynchronous and phasic release appeared to compete for a common limited supply of release-ready quanta because agents that block asynchronous release, such as EGTA-AM, led to enhanced steady-state phasic release, whereas prolongation of the asynchronous release time course by LiCl delayed recovery of phasic release from depression. Modeling suggested that the resistance of asynchronous release to depression was associated with its ability to out-compete phasic release for recovered quanta attributable to its relatively low release rate (up to 0.04/msec per vesicle) stimulated by bulk intracellular Ca2+ concentration ([Ca2+]i) that could function over prolonged intervals between successive stimuli. Although phasic release was associated with a considerably higher peak rate of release (0.4/msec per vesicle), the [Ca2+]i microdomains that trigger it are brief (1 msec), and with asynchronous release present, relatively few quanta can accumulate within the RRP to be available for phasic release. We conclude that despite depression of phasic release during train stimulation, transmission can be maintained at a near-maximal rate by switching to an asynchronous mode that takes advantage of a bulk presynaptic [Ca2+]i.
Key words: short-term synaptic depression; asynchronous release; phasic release; glutamatergic; readily releasable pool; release probability; hippocampus; autapse
Received Oct 1, 2003; revised November 5, 2003; accepted November 5, 2003.
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