QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, June 23, 2004, 24(25):5684-5693; doi:10.1523/JNEUROSCI.0492-04.2004

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (17) Citing Articles via Google Scholar Google Scholar Articles by Dhingra, A. Articles by Vardi, N. Search for Related Content PubMed PubMed Citation Articles by Dhingra, A. Articles by Vardi, N.

 Previous Article  |  Next Article 

Cellular/Molecular
A Retinal-Specific Regulator of G-Protein Signaling Interacts with G_o and Accelerates an Expressed Metabotropic Glutamate Receptor 6 Cascade

Anuradha Dhingra,¹ Eva Faurobert,² Nathan Dascal,³ Peter Sterling,¹ and Noga Vardi¹

¹Department of Neuroscience, University of Pennsylvania, Philadelphia, Pennsylvania 19104, ²Institut de Pharmacologie Moleculaire et Cellulaire, Centre National de la Recherche Scientifique Unité Propre de Recherche, 06560 Valbonne, France, and ³Department of Physiology and Pharmacology, Tel Aviv University, Tel Aviv 69978, Israel

G_o is the most abundant G-protein in the brain, but its regulators are essentially unknown. In retina, G_o1 is obligatory in mediating the metabotropic glutamate receptor 6 (mGluR6)-initiated ON response. To identify the interactors of G_o, we conducted a yeast two-hybrid screen with constituitively active G_o as a bait. The screen frequently identified a regulator of G-protein signaling (RGS), Ret-RGS1, the interaction of which we confirmed by coimmunoprecipitation with G_o in transfected cells and in retina. Ret-RGS1 localized to the dendritic tips of ON bipolar neurons, along with mGluR6 and G_o1. When Ret-RGS1 was coexpressed in Xenopus oocytes with mGluR6, G_o1, and a GIRK (G-protein-gated inwardly rectifying K⁺) channel, it accelerated the deactivation of the channel response to glutamate in a concentration-dependent manner. Because light onset suppresses glutamate release from photoreceptors onto the ON bipolar dendrites, Ret-RGS1 should accelerate the rising phase of the light response of the ON bipolar cell. This would tend to match its kinetics to that of the OFF bipolar that arises directly from ligand-gated channels.

Key words: ON bipolar neuron; retina; G_o; Ret-RGS1; GTPase-activating protein; GIRK channel

---

Received Feb 11, 2004; revised April 16, 2004; accepted May 7, 2004.

This article has been cited by other articles:

				D. Beqollari, M. J. Betzenhauser, and P. J. Kammermeier Altered G-Protein Coupling in an mGluR6 Point Mutant Associated with Congenital Stationary Night Blindness Mol. Pharmacol., November 1, 2009; 76(5): 992 - 997. [Abstract] [Full Text] [PDF]

				Y. Cao, I. Masuho, H. Okawa, K. Xie, J. Asami, P. J. Kammermeier, D. M. Maddox, T. Furukawa, T. Inoue, A. P. Sampath, et al. Retina-Specific GTPase Accelerator RGS11/G{beta}5S/R9AP Is a Constitutive Heterotrimer Selectively Targeted to mGluR6 in ON-Bipolar Neurons J. Neurosci., July 22, 2009; 29(29): 9301 - 9313. [Abstract] [Full Text] [PDF]

				D. K. Mojumder, Y. Qian, and T. G. Wensel Two R7 Regulator of G-Protein Signaling Proteins Shape Retinal Bipolar Cell Signaling J. Neurosci., June 17, 2009; 29(24): 7753 - 7765. [Abstract] [Full Text] [PDF]

				Y. Nakajima, M. Moriyama, M. Hattori, N. Minato, and S. Nakanishi Isolation of ON Bipolar Cell Genes via hrGFP-coupled Cell Enrichment Using the mGluR6 Promoter J. Biochem., June 1, 2009; 145(6): 811 - 818. [Abstract] [Full Text] [PDF]

				Y. Xu, P. Sulaiman, R. M. Feddersen, J. Liu, R. G. Smith, and N. Vardi Retinal ON Bipolar Cells Express a New PCP2 Splice Variant That Accelerates the Light Response J. Neurosci., September 3, 2008; 28(36): 8873 - 8884. [Abstract] [Full Text] [PDF]

				A. Rao, R. Dallman, S. Henderson, and C.-K. Chen G 5 Is Required for Normal Light Responses and Morphology of Retinal ON-Bipolar Cells J. Neurosci., December 19, 2007; 27(51): 14199 - 14204. [Abstract] [Full Text] [PDF]

				T. A. John, B. O. Ibe, and J. Usha Raj Oxygen alters caveolin-1 and nitric oxide synthase-3 functions in ovine fetal and neonatal lung microvascular endothelial cells Am J Physiol Lung Cell Mol Physiol, November 1, 2006; 291(5): L1079 - L1093. [Abstract] [Full Text] [PDF]

				N. Wettschureck and S. Offermanns Mammalian G Proteins and Their Cell Type Specific Functions Physiol Rev, October 1, 2005; 85(4): 1159 - 1204. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help