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The Journal of Neuroscience, July 28, 2004, 24(30):6703-6714; doi:10.1523/JNEUROSCI.0307-04.2004
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Cellular/Molecular
Voltage Imaging from Dendrites of Mitral Cells: EPSP Attenuation and Spike Trigger Zones
Maja Djurisic,1,3 Srdjan Antic,1,2,3 Wei R. Chen,2,3 andDejan Zecevic1,3 Departments of 1Cellular and Molecular Physiology and 2Neurobiology, Yale University School of Medicine, New Haven, Connecticut 06520, and 3Marine Biological Laboratory, Woods Hole, Massachusetts 02543
To obtain a more complete description of individual neurons, it is necessary to complement the electrical patch pipette measurements with technologies that permit a massive parallel recording from many sites on neuronal processes. This can be achieved by using voltage imaging with intracellular dyes. With this approach, we investigated the functional structure of a mitral cell, the principal output neuron in the rat olfactory bulb. The most significant finding concerns the characteristics of EPSPs at the synaptic sites and surprisingly small attenuation along the trunk of the primary dendrite. Also, the experiments were performed to determine the number, location, and stability of spike trigger zones, the excitability of terminal dendritic branches, and the pattern and nature of spike initiation and propagation in the primary and secondary dendrites. The results show that optical data can be used to deduce the amplitude and shape of the EPSPs evoked by olfactory nerve stimulation at the site of origin (glomerular tuft) and to determine its attenuation along the entire length of the primary dendrite. This attenuation corresponds to an unusually large mean apparent "length constant" of the primary dendrite. Furthermore, the images of spike trigger zones showed that an action potential can be initiated in three different compartments of the mitral cell: the soma-axon region, the primary dendrite trunk, and the terminal dendritic tuft, which appears to be fully excitable. Finally, secondary dendrites clearly support the active propagation of action potentials.
Key words: mitral cells; voltage imaging; voltage-sensitive dyes; dendrites; synaptic integration; olfaction
Received Jan 27, 2004; revised June 10, 2004; accepted June 10, 2004.
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