QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, October 13, 2004, 24(41):9059-9066; doi:10.1523/JNEUROSCI.1645-04.2004

This Article Full Text Full Text (PDF) HTML Page - index.htslp Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (60) Citing Articles via Google Scholar Google Scholar Articles by Gong, Z. Articles by Kim, C. Search for Related Content PubMed PubMed Citation Articles by Gong, Z. Articles by Kim, C.

 Previous Article  |  Next Article 

Cellular/Molecular
Two Interdependent TRPV Channel Subunits, Inactive and Nanchung, Mediate Hearing in Drosophila

Zhefeng Gong,^1 * Wonseok Son,^2 * Yun Doo Chung,^3 * Janghwan Kim,^2,6 Dong Wook Shin,² Colleen A. McClung,¹ Yong Lee,⁴ Hye Won Lee,² Deok-Jin Chang,⁴ Bong-Kiun Kaang,⁴ Hawon Cho,⁵ Uhtaek Oh,⁵ Jay Hirsh,¹ Maurice J. Kernan,³ and Changsoo Kim^2,7

¹Biology Department, University of Virginia, Charlottesville, Virginia 22904, ²Department of Genetics, Hanwha Chemical Company R&D Center, Sinsung-Dong, Yusung-Gu, Daejeon 305-345, Korea, ³Department of Neurobiology and Behavior, State University of New York at Stony Brook, New York 11794, ⁴National Research Laboratory, School of Biological Sciences, Seoul National University, Seoul 151-742, Korea, ⁵Sensory Research Center, Creative Research Initiatives, Seoul National University, Seoul 151-742, Korea, ⁶Department of Microbiology, Chungnam National University, Daejeon 305-764, Korea, and ⁷School of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Korea

Hearing in Drosophila depends on the transduction of antennal vibration into receptor potentials by ciliated sensory neurons in Johnston's organ, the antennal chordotonal organ. We previously found that a Drosophila protein in the vanilloid receptor subfamily (TRPV) channel subunit, Nanchung (NAN), is localized to the chordotonal cilia and required to generate sound-evoked potentials (Kim et al., 2003). Here, we show that the only other Drosophila TRPV protein is mutated in the behavioral mutant inactive (iav). The IAV protein forms a hypotonically activated channel when expressed in cultured cells; in flies, it is specifically expressed in the chordotonal neurons, localized to their cilia and required for hearing. IAV and NAN are each undetectable in cilia of mutants lacking the other protein, indicating that they both contribute to a heteromultimeric transduction channel in vivo. A functional green fluorescence protein-IAV fusion protein shows that the channel is restricted to the proximal cilium, constraining models for channel activation.

Key words: channel; deafness; Drosophila; hair cell; hearing; mechanosensory

---

Received April 29, 2004; revised August 4, 2004; accepted August 4, 2004.

This article has been cited by other articles:

				Q. Lu, P. R. Senthilan, T. Effertz, B. Nadrowski, and M. C. Gopfert Using Drosophila for studying fundamental processes in hearing Integr. Comp. Biol., December 1, 2009; 49(6): 674 - 680. [Abstract] [Full Text] [PDF]

				Y. Sun, L. Liu, Y. Ben-Shahar, J. S. Jacobs, D. F. Eberl, and M. J. Welsh TRPA channels distinguish gravity sensing from hearing in Johnston's organ PNAS, August 11, 2009; 106(32): 13606 - 13611. [Abstract] [Full Text] [PDF]

				A. Crocker and A. Sehgal Octopamine Regulates Sleep in Drosophila through Protein Kinase A-Dependent Mechanisms J. Neurosci., September 17, 2008; 28(38): 9377 - 9385. [Abstract] [Full Text] [PDF]

				H. Ruan and C.-F. Wu Social interaction-mediated lifespan extension of Drosophila Cu/Zn superoxide dismutase mutants PNAS, May 27, 2008; 105(21): 7506 - 7510. [Abstract] [Full Text] [PDF]

				C. Grimm, M. P. Cuajungco, A. F. J. van Aken, M. Schnee, S. Jors, C. J. Kros, A. J. Ricci, and S. Heller A helix-breaking mutation in TRPML3 leads to constitutive activity underlying deafness in the varitint-waddler mouse PNAS, December 4, 2007; 104(49): 19583 - 19588. [Abstract] [Full Text] [PDF]

				A. M. Jose, I. A. Bany, D. L. Chase, and M. R. Koelle A Specific Subset of Transient Receptor Potential Vanilloid-Type Channel Subunits in Caenorhabditis elegans Endocrine Cells Function as Mixed Heteromers to Promote Neurotransmitter Release Genetics, January 1, 2007; 175(1): 93 - 105. [Abstract] [Full Text] [PDF]

				W. Liedtke Transient receptor potential vanilloid channels functioning in transduction of osmotic stimuli J. Endocrinol., December 1, 2006; 191(3): 515 - 523. [Abstract] [Full Text] [PDF]

				Y.-K. Bae, H. Qin, K. M. Knobel, J. Hu, J. L. Rosenbaum, and M. M. Barr General and cell-type specific mechanisms target TRPP2/PKD-2 to cilia Development, October 1, 2006; 133(19): 3859 - 3870. [Abstract] [Full Text] [PDF]

				V. Singla and J. F. Reiter The primary cilium as the cell's antenna: signaling at a sensory organelle. Science, August 4, 2006; 313(5787): 629 - 633. [Abstract] [Full Text] [PDF]

				S. H. Cole, G. E. Carney, C. A. McClung, S. S. Willard, B. J. Taylor, and J. Hirsh Two Functional but Noncomplementing Drosophila Tyrosine Decarboxylase Genes: DISTINCT ROLES FOR NEURAL TYRAMINE AND OCTOPAMINE IN FEMALE FERTILITY J. Biol. Chem., April 15, 2005; 280(15): 14948 - 14955. [Abstract] [Full Text] [PDF]

				C. Montell The TRP Superfamily of Cation Channels Sci. Signal., February 22, 2005; 2005(272): re3 - re3. [Abstract] [Full Text] [PDF]

				M. Rosenzweig, K. M. Brennan, T. D. Tayler, P. O. Phelps, A. Patapoutian, and P. A. Garrity The Drosophila ortholog of vertebrate TRPA1 regulates thermotaxis Genes & Dev., February 15, 2005; 19(4): 419 - 424. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help