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The Journal of Neuroscience, October 27, 2004, 24(43):9521-9530; doi:10.1523/JNEUROSCI.2639-04.2004

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Cellular/Molecular
TRPV1 Function in Mouse Colon Sensory Neurons Is Enhanced by Metabotropic 5-Hydroxytryptamine Receptor Activation

Takeshi Sugiuar,^1,2 Klaus Bielefeldt,³ and G. F. Gebhart¹

¹Department of Pharmacology, Carver College of Medicine, The University of Iowa, Iowa City, Iowa 52242, ²Department of Anesthesiology and Medical Crisis Management, Nagoya City University Graduate School Medical Sciences, Nagoya 467-8601, Japan, and ³Division of Gastroenterology, Department of Internal Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261

Using whole-cell patch-clamp methods, we examined the hypothesis that serotonin [5-hydroxytryptamine (5-HT)] receptor activation enhances TRPV1 function in mouse colon sensory neurons in lumbosacral dorsal root ganglia, which were identified by retrograde labeling with DiI (1,1'-dioctadecyl-3,3,3',3-tetramethlindocarbocyanine methanesulfonate) injected into multiple sites in the wall of the descending colon. 5-HT increased membrane excitability at a temperature below body temperature in response to thermal ramp stimuli in colon sensory neurons from wild-type mice, but not from TRPV1 knock-out mice. 5-HT significantly enhanced capsaicin-, heat-, and proton-evoked currents with an EC₅₀ value of 2.2 µM. 5-HT (1 µM) significantly increased capsaicin-evoked (100 nM) and proton-evoked (pH 5.5) currents 1.6- and 4.7-fold, respectively, and significantly decreased the threshold temperature for heat current activation from 42 to 38°C. The enhancement of TRPV1 by 5-HT was significantly attenuated by selective 5-HT₂ and 5-HT₄ receptor antagonists, but not by a 5-HT₃ receptor antagonist. In support, 5-HT₂ and 5-HT₄ receptor agonists mimicked the facilitating effects of 5-HT on TRPV1 function. Downstream signaling required G-protein activation and phosphorylation as intracellularly administered GDP--S [guanosine 5'-O-(2-thiodiphosphate], protein kinase A inhibitors, and an A-kinase anchoring protein inhibitor significantly blocked serotonergic facilitation of TRPV1 function; 5-HT₂ receptor-mediated facilitation was also inhibited by a PKC inhibitor. We conclude that the facilitation of TRPV1 by metabotropic 5-HT receptor activation may contribute to hypersensitivity of primary afferent neurons in irritable bowel syndrome patients.

Key words: visceral pain; thermal hyperalgesia; DRG; serotonin receptor; capsaicin receptor; DiI

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Received July 2, 2004; revised August 5, 2004; accepted September 1, 2004.

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