QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, October 27, 2004, 24(43):9612-9622; doi:10.1523/JNEUROSCI.5583-03.2004

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (18) Citing Articles via Google Scholar Google Scholar Articles by Unni, V. K. Articles by Siegelbaum, S. A. Search for Related Content PubMed PubMed Citation Articles by Unni, V. K. Articles by Siegelbaum, S. A.

 Previous Article  |  Next Article 

Cellular/Molecular
Calcium Release from Presynaptic Ryanodine-Sensitive Stores Is Required for Long-Term Depression at Hippocampal CA3-CA3 Pyramidal Neuron Synapses

Vivek K. Unni,¹ Stanislav S. Zakharenko,¹ Leonard Zablow,^1,3 Anthony J. DeCostanzo,² and Steven A. Siegelbaum^1,2,3

¹Center for Neurobiology and Behavior, ²Department of Pharmacology, and ³Howard Hughes Medical Institute, Columbia University, New York, New York 10032

Although Ca²⁺ release from internal stores has been proposed to be important for the induction of long-term synaptic plasticity, the importance of Ca²⁺ stores localized in presynaptic terminals remains unclear. Here, we have selectively applied pharmacological antagonists to either the presynaptic or postsynaptic cell in paired whole-cell recordings from hippocampal CA3 pyramidal neurons in slice culture. We demonstrate directly the necessary role of presynaptic, but not postsynaptic, ryanodine-sensitive Ca²⁺ stores in the induction of NMDA receptor (NMDAR)-dependent long-term depression (LTD). Using two-photon laser scanning microscopy, we further find that release from the ryanodine-sensitive stores during prolonged synaptic stimulation generates a slowly rising Ca²⁺ signal in the presynaptic terminal that is required for the induction of LTD. Moreover, this form of LTD has a significant presynaptic component of expression because it causes a marked decrease in the rate of release from CA3 neuron presynaptic terminals of FM 1-43, a fluorescent probe of synaptic vesicle cycling. Thus, Ca²⁺ release from presynaptic ryanodine-sensitive stores is critical in the induction of a presynaptic component of NMDAR-dependent LTD.

Key words: long-term depression; CA3; internal calcium stores; ryanodine-sensitive stores; hippocampus; synaptic plasticity

---

Received Dec 18, 2003; revised September 7, 2004; accepted September 9, 2004.

This article has been cited by other articles:

				H. Shimizu, M. Fukaya, M. Yamasaki, M. Watanabe, T. Manabe, and H. Kamiya Use-dependent amplification of presynaptic Ca2+ signaling by axonal ryanodine receptors at the hippocampal mossy fiber synapse PNAS, August 19, 2008; 105(33): 11998 - 12003. [Abstract] [Full Text] [PDF]

				P. J. Sjostrom, E. A. Rancz, A. Roth, and M. Hausser Dendritic Excitability and Synaptic Plasticity Physiol Rev, April 1, 2008; 88(2): 769 - 840. [Abstract] [Full Text] [PDF]

				R. A. Nichols, A. F. Dengler, E. M. Nakagawa, M. Bashkin, B. T. Paul, J. Wu, and G. M. Khan A Constitutive, Transient Receptor Potential-like Ca2+ Influx Pathway in Presynaptic Nerve Endings Independent of Voltage-gated Ca2+ Channels and Na+/Ca2+ Exchange J. Biol. Chem., December 7, 2007; 282(49): 36102 - 36111. [Abstract] [Full Text] [PDF]

				C. Supnet, J. Grant, H. Kong, D. Westaway, and M. Mayne Amyloid-beta-(1-42) Increases Ryanodine Receptor-3 Expression and Function in Neurons of TgCRND8 Mice J. Biol. Chem., December 15, 2006; 281(50): 38440 - 38447. [Abstract] [Full Text] [PDF]

				V. De Crescenzo, K. E. Fogarty, R. ZhuGe, R. A. Tuft, L. M. Lifshitz, J. Carmichael, K. D. Bellve, S. P. Baker, S. Zissimopoulos, F. A. Lai, et al. Dihydropyridine receptors and type 1 ryanodine receptors constitute the molecular machinery for voltage-induced Ca2+ release in nerve terminals. J. Neurosci., July 19, 2006; 26(29): 7565 - 7574. [Abstract] [Full Text] [PDF]

				V. A. Bender, K. J. Bender, D. J. Brasier, and D. E. Feldman Two coincidence detectors for spike timing-dependent plasticity in somatosensory cortex. J. Neurosci., April 19, 2006; 26(16): 4166 - 4177. [Abstract] [Full Text] [PDF]

				R. ZhuGe, V. DeCrescenzo, V. Sorrentino, F. A. Lai, R. A. Tuft, L. M. Lifshitz, J. R. Lemos, C. Smith, K. E. Fogarty, and J. V. Walsh Jr. Syntillas Release Ca2+ at a Site Different from the Microdomain Where Exocytosis Occurs in Mouse Chromaffin Cells Biophys. J., March 15, 2006; 90(6): 2027 - 2037. [Abstract] [Full Text] [PDF]

				F.-M. Lu and R. D. Hawkins Presynaptic and postsynaptic Ca2+ and CamKII contribute to long-term potentiation at synapses between individual CA3 neurons. PNAS, March 14, 2006; 103(11): 4264 - 4269. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help