QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, December 1, 2004, 24(48):10900-10907; doi:10.1523/JNEUROSCI.2240-04.2004

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (10) Citing Articles via Google Scholar Google Scholar Articles by Ohtsuki, G. Articles by Hirano, T. Search for Related Content PubMed PubMed Citation Articles by Ohtsuki, G. Articles by Hirano, T.

 Previous Article  |  Next Article 

Cellular/Molecular
Enhanced Inhibitory Synaptic Transmission in the Cerebellar Molecular Layer of the GluR2 Knock-Out Mouse

Gen Ohtsuki,^1,2 Shin-ya Kawaguchi,^1,2 Masayoshi Mishina,³ and Tomoo Hirano^1,2

¹Department of Biophysics, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan, ²Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Kawaguchi 332-0012, Japan, and ³Department of Molecular Neurobiology and Pharmacology, School of Medicine, University of Tokyo, and Solution-Oriented Research for Science and Technology, Tokyo 113-0033, Japan

A novel ionotropic glutamate receptor subunit 2 (GluR2), which is specifically expressed in cerebellar Purkinje neurons (PNs), is implicated in the induction of long-term depression. Mutant mice deficient in GluR2 (2^-/-) have abnormal cerebellar synaptic organization and impaired motor coordination and learning. Previous in vivo extracellular recordings in2^-/- revealed that PN activity distinct from that in wild-type (WT) mice is attributable to enhanced climbing fiber activity. Here, we report that GABAergic synaptic transmission was enhanced in the molecular layer of the cerebellar cortex in 2^-/-. Optical recordings in cerebellar slice preparations indicated that application of bicuculline, a GABA_A receptor antagonist, increased the amplitude and area of excitation propagation more in 2^-/- than in WT. Whole-cell patch-clamp recordings from PNs demonstrated that miniature IPSC (mIPSC) amplitude were larger in 2^-/- than in WT. Also, rebound potentiation (RP), a type of long-lasting inhibitory synaptic potentiation inducible by postsynaptic depolarization of PNs in WT, was not induced in slices prepared from 2^-/-. In contrast, RP was induced in cultured PNs prepared from 2^-/-. Pharmacologic activation of climbing fibers in WT in vivo increased mIPSC amplitudes in PNs and prevented RP induction. These results suggest that enhanced climbing fiber activity in 2^-/- potentiates IPSC amplitudes in PNs through RP in vivo, resulting in the prevention of additional RP induction.

Key words: cerebellum; Purkinje cell; receptor; glutamate; inhibitory synapse; synaptic plasticity

---

Received June 9, 2004; revised September 24, 2004; accepted October 18, 2004.

This article has been cited by other articles:

				N. H. Barmack and V. Yakhnitsa Functions of Interneurons in Mouse Cerebellum J. Neurosci., January 30, 2008; 28(5): 1140 - 1152. [Abstract] [Full Text] [PDF]

				W. Mittmann and M. Hausser Linking Synaptic Plasticity and Spike Output at Excitatory and Inhibitory Synapses onto Cerebellar Purkinje Cells J. Neurosci., May 23, 2007; 27(21): 5559 - 5570. [Abstract] [Full Text] [PDF]

				J.-M. Fritschy, P. Panzanelli, J. E. Kralic, K. E. Vogt, and M. Sassoe-Pognetto Differential dependence of axo-dendritic and axo-somatic GABAergic synapses on GABAA receptors containing the alpha1 subunit in Purkinje cells. J. Neurosci., March 22, 2006; 26(12): 3245 - 3255. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help