 |
||||
|
||||
|
||||
|
||||
The Journal of Neuroscience, February 11, 2004, 24(6):1468-1477; doi:10.1523/JNEUROSCI.3661-03.2004
Previous Article | Next Article
Cellular/Molecular
Novel Features of Cryptochrome-Mediated Photoreception in the Brain Circadian Clock of Drosophila
André Klarsfeld, Sébastien Malpel, Christine Michard-Vanhée, Marie Picot, Elisabeth Chélot, andFrançois Rouyer Institut de Neurobiologie Alfred Fessard, Centre National de la Recherche Scientifique, Unité Propre de Recherche 2216, 91198 Gif-sur-Yvette, France
In Drosophila, light affects circadian behavioral rhythms via at least two distinct mechanisms. One of them relies on the visual phototransduction cascade. The other involves a presumptive photopigment, cryptochrome (cry), expressed in lateral brain neurons that control behavioral rhythms. We show here that cry is expressed in most, if not all, larval and adult neuronal groups expressing the PERIOD (PER) protein, with the notable exception of larval dorsal neurons (DN2s) in which PER cycles in antiphase to all other known cells. Forcing cry expression in the larval DN2s gave them a normal phase of PER cycling, indicating that their unique antiphase rhythm is related to their lack of cry expression. We were able to directly monitor CRY protein in Drosophila brains in situ. It appeared highly unstable in the light, whereas in the dark, it accumulated in both the nucleus and the cytoplasm, including some neuritic projections. We also show that dorsal PER-expressing brain neurons, the adult DN1s, are the only brain neurons to coexpress the CRY protein and the photoreceptor differentiation factor GLASS. Studies of various visual system mutants and their combination with the cryb mutation indicated that the adult DN1s contribute significantly to the light sensitivity of the clock controlling activity rhythms, and that this contribution depends on CRY. Moreover, all CRY-independent light inputs into this central behavioral clock were found to require the visual system. Finally, we show that the photoreceptive DN1 neurons do not behave as autonomous oscillators, because their PER oscillations in constant darkness rapidly damp out in the absence of pigment-dispersing-factor signaling from the ventral lateral neurons.
Key words: locomotor activity rhythms; entrainment; circadian photoreception; cryptochrome; GLASS protein; PERIOD protein; pigment-dispersing factor; dorsal neurons
Received Aug 5, 2003; revised December 10, 2003; accepted December 11, 2003.
This article has been cited by other articles:
Hao Zheng, F. Ng, Yixiao Liu, and P. E. Hardin
Spatial and Circadian Regulation of cry in Drosophila
J Biol Rhythms, August 1, 2008; 23(4): 283 - 295.
[Abstract] [PDF]
J. Benito, J. H. Houl, G. W. Roman, and P. E. Hardin
The Blue-Light Photoreceptor CRYPTOCHROME Is Expressed in a Subset of Circadian Oscillator Neurons in the Drosophila CNS
J Biol Rhythms, August 1, 2008; 23(4): 296 - 307.
[Abstract] [PDF]
B. Richier, C. Michard-Vanhee, A. Lamouroux, C. Papin, and F. Rouyer
The Clockwork Orange Drosophila Protein Functions as Both an Activator and a Repressor of Clock Gene Expression
J Biol Rhythms, April 1, 2008; 23(2): 103 - 116.
[Abstract] [PDF]
V. Sheeba, H. Gu, V. K. Sharma, D. K. O'Dowd, and T. C. Holmes
Circadian- and Light-Dependent Regulation of Resting Membrane Potential and Spontaneous Action Potential Firing of Drosophila Circadian Pacemaker Neurons
J Neurophysiol, February 1, 2008; 99(2): 976 - 988.
[Abstract] [Full Text] [PDF]
E. Dolezelova, D. Dolezel, and J. C. Hall
Rhythm Defects Caused by Newly Engineered Null Mutations in Drosophila's cryptochrome Gene
Genetics, September 1, 2007; 177(1): 329 - 345.
[Abstract] [Full Text] [PDF]
M. d. l. P. Fernandez, J. Chu, A. Villella, N. Atkinson, S. A. Kay, and M. F. Ceriani
Impaired clock output by altered connectivity in the circadian network
PNAS, March 27, 2007; 104(13): 5650 - 5655.
[Abstract] [Full Text] [PDF]
S. Veleri, D. Rieger, C. Helfrich-Forster, and R. Stanewsky
Hofbauer-Buchner Eyelet Affects Circadian Photosensitivity and Coordinates TIM and PER Expression in Drosophila Clock Neurons
J Biol Rhythms, February 1, 2007; 22(1): 29 - 42.
[Abstract] [PDF]
F. T. Glaser and R. Stanewsky
Synchronization of the Drosophila Circadian Clock by Temperature Cycles
Cold Spring Harb Symp Quant Biol, January 1, 2007; 72(0): 233 - 242.
[Abstract] [PDF]
C. Helfrich-Forster, T. Yoshii, C. Wulbeck, E. Grieshaber, D. Rieger, W. Bachleitner, P. Cusumano, and F. Rouyer
The Lateral and Dorsal Neurons of Drosophila melanogaster: New Insights about Their Morphology and Function
Cold Spring Harb Symp Quant Biol, January 1, 2007; 72(0): 517 - 525.
[Abstract] [PDF]
P. H. Taghert and O. T. Shafer
Mechanisms of Clock Output in the Drosophila Circadian Pacemaker System.
J Biol Rhythms, December 1, 2006; 21(6): 445 - 457.
[Abstract] [PDF]
K. D. Williams, M. Busto, M. L. Suster, A. K.-C. So, Y. Ben-Shahar, S. J. Leevers, and M. B. Sokolowski
Natural variation in Drosophila melanogaster diapause due to the insulin-regulated PI3-kinase
PNAS, October 24, 2006; 103(43): 15911 - 15915.
[Abstract] [Full Text] [PDF]
B. L. Rush, A. Murad, P. Emery, and J. M. Giebultowicz
Ectopic CRYPTOCHROME Renders TIM Light Sensitive in the Drosophila Ovary.
J Biol Rhythms, August 1, 2006; 21(4): 272 - 278.
[Abstract] [PDF]
|
|
|
|
 |
|