WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
The Journal of Neuroscience, January 5, 2005, 25(1):260-270; doi:10.1523/JNEUROSCI.3165-04.2005

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (48)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sara, Y.
Right arrow Articles by Kavalali, E. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sara, Y.
Right arrow Articles by Kavalali, E. T.

 Previous Article

Cellular/Molecular
Selective Capability of SynCAM and Neuroligin for Functional Synapse Assembly

Yildirim Sara,1 Thomas Biederer,1,5 Deniz Atasoy,1 Alexander Chubykin,1 Marina G. Mozhayeva,1 Thomas C. Südhof,1,2,4 and Ege T. Kavalali1,3

1Center for Basic Neuroscience, Departments of 2Molecular Genetics and 3Physiology, and 4Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center, Dallas, Texas 75390-9111, and 5Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520-8024

Synaptic cell adhesion is central for synapse formation and function. Recently, the synaptic cell adhesion molecules neuroligin 1 (NL1) and SynCAM were shown to induce presynaptic differentiation in cocultured neurons when expressed in a non-neuronal cell. However, it is uncertain how similar the resulting artificial synapses are to regular synapses. Are these molecules isofunctional, or do all neuronal cell adhesion molecules nonspecifically activate synapse formation? To address these questions, we analyzed the properties of artificial synapses induced by NL1 and SynCAM, compared the actions of these molecules with those of other neuronal cell adhesion molecules, and examined the functional effects of NL1 and SynCAM overexpression in neurons. We found that only NL1 and SynCAM specifically induced presynaptic differentiation in cocultured neurons. The induced nerve terminals were capable of both spontaneous and evoked neurotransmitter release, suggesting that a full secretory apparatus was assembled. By all measures, SynCAM- and NL1-induced artificial synapses were identical. Overexpression in neurons demonstrated that only SynCAM, but not NL1, increased synaptic function in immature developing excitatory neurons after 8 d in vitro. Tests of chimeric molecules revealed that the dominant-positive effect of SynCAM on synaptic function in developing neurons was mediated by its intracellular cytoplasmic tail. Interestingly, morphological analysis of neurons overexpressing SynCAM or NL1 showed the opposite of the predictions from electrophysiological results. In this case, only NL1 increased the synapse number, suggesting a role for NL1 in morphological synapse induction. These results suggest that both NL1 and SynCAM act similarly and specifically in artificial synapse induction but that this process does not reflect a shared physiological function of these molecules.

Key words: neuroligin; SynCAM; synaptogenesis; hippocampal neuron; cell adhesion; neurotransmitter release

Received Aug 2, 2004; revised October 28, 2004; accepted November 12, 2004.




This article has been cited by other articles:


Home page
 J. Neurosci.Home page
A. L. Lucido, F. Suarez Sanchez, P. Thostrup, A. V. Kwiatkowski, S. Leal-Ortiz, G. Gopalakrishnan, D. Liazoghli, W. Belkaid, R. B. Lennox, P. Grutter, et al.
Rapid Assembly of Functional Presynaptic Boutons Triggered by Adhesive Contacts
J. Neurosci., October 7, 2009; 29(40): 12449 - 12466.
[Abstract] [Full Text] [PDF]

Home page
 Cold Spring Harb. Perspect. Biol.Home page
N. Giagtzoglou, C. V. Ly, and H. J. Bellen
Cell Adhesion, the Backbone of the Synapse: "Vertebrate" and "Invertebrate" Perspectives
Cold Spring Harb Perspect Biol, October 1, 2009; 1(4): a003079 - a003079.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
Z. Wang, B. Wang, L. Yang, Q. Guo, N. Aithmitti, Z. Songyang, and H. Zheng
Presynaptic and Postsynaptic Interaction of the Amyloid Precursor Protein Promotes Peripheral and Central Synaptogenesis
J. Neurosci., September 2, 2009; 29(35): 10788 - 10801.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. Wittenmayer, C. Korber, H. Liu, T. Kremer, F. Varoqueaux, E. R. Chapman, N. Brose, T. Kuner, and T. Dresbach
Postsynaptic Neuroligin1 regulates presynaptic maturation
PNAS, August 11, 2009; 106(32): 13564 - 13569.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Heine, O. Thoumine, M. Mondin, B. Tessier, G. Giannone, and D. Choquet
Activity-independent and subunit-specific recruitment of functional AMPA receptors at neurexin/neuroligin contacts
PNAS, December 30, 2008; 105(52): 20947 - 20952.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
A. T. Suckow, D. Comoletti, M. A. Waldrop, M. Mosedale, S. Egodage, P. Taylor, and S. D. Chessler
Expression of Neurexin, Neuroligin, and Their Cytoplasmic Binding Partners in the Pancreatic {beta}-Cells and the Involvement of Neuroligin in Insulin Secretion
Endocrinology, December 1, 2008; 149(12): 6006 - 6017.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. Park, B. Liu, T. Chen, H. Li, X. Hu, J. Gao, Y. Zhu, Q. Zhu, B. Qiang, J. Yuan, et al.
Disruption of Nectin-Like 1 Cell Adhesion Molecule Leads to Delayed Axonal Myelination in the CNS
J. Neurosci., November 26, 2008; 28(48): 12815 - 12819.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. W. Lyckman, S. Horng, C. A. Leamey, D. Tropea, A. Watakabe, A. Van Wart, C. McCurry, T. Yamamori, and M. Sur
Gene expression patterns in visual cortex during the critical period: Synaptic stabilization and reversal by visual deprivation
PNAS, July 8, 2008; 105(27): 9409 - 9414.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
R. M. Hines, L. Wu, D. J. Hines, H. Steenland, S. Mansour, R. Dahlhaus, R. R. Singaraja, X. Cao, E. Sammler, S. G. Hormuzdi, et al.
Synaptic Imbalance, Stereotypies, and Impaired Social Interactions in Mice with Altered Neuroligin 2 Expression
J. Neurosci., June 11, 2008; 28(24): 6055 - 6067.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Kang, X. Zhang, F. Dobie, H. Wu, and A. M. Craig
Induction of GABAergic Postsynaptic Differentiation by {alpha}-Neurexins
J. Biol. Chem., January 25, 2008; 283(4): 2323 - 2334.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
A. I. Fogel, M. R. Akins, A. J. Krupp, M. Stagi, V. Stein, and T. Biederer
SynCAMs Organize Synapses through Heterophilic Adhesion
J. Neurosci., November 14, 2007; 27(46): 12516 - 12530.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J.-Y. Hu, Y. Chen, and S. Schacher
Multifunctional Role of Protein Kinase C in Regulating the Formation and Maturation of Specific Synapses
J. Neurosci., October 24, 2007; 27(43): 11712 - 11724.
[Abstract] [Full Text] [PDF]

Home page
 Sci SignalHome page
K. Gerrow and A. El-Husseini
Receptors Look Outward: Revealing Signals That Bring Excitation to Synapses
Sci. Signal., October 16, 2007; 2007(408): pe56 - pe56.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
J. Rohrbough, E. Rushton, E. Woodruff III, T. Fergestad, K. Vigneswaran, and K. Broadie
Presynaptic establishment of the synaptic cleft extracellular matrix is required for post-synaptic differentiation
Genes & Dev., October 15, 2007; 21(20): 2607 - 2628.
[Abstract] [Full Text] [PDF]

Home page
 NeuroscientistHome page
B. Lardi-Studler and J.-M. Fritschy
Matching of Pre- and Postsynaptic Specializations during Synaptogenesis
Neuroscientist, April 1, 2007; 13(2): 115 - 126.
[Abstract] [PDF]

Home page
 J. Neurosci.Home page
H. Taniguchi, L. Gollan, F. G. Scholl, V. Mahadomrongkul, E. Dobler, N. Limthong, M. Peck, C. Aoki, and P. Scheiffele
Silencing of Neuroligin Function by Postsynaptic Neurexins
J. Neurosci., March 14, 2007; 27(11): 2815 - 2824.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
D. J. Solecki, E.-E. Govek, T. Tomoda, and M. E. Hatten
Neuronal polarity in CNS development
Genes & Dev., October 1, 2006; 20(19): 2639 - 2647.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J.-M. Fritschy, P. Panzanelli, J. E. Kralic, K. E. Vogt, and M. Sassoe-Pognetto
Differential dependence of axo-dendritic and axo-somatic GABAergic synapses on GABAA receptors containing the alpha1 subunit in Purkinje cells.
J. Neurosci., March 22, 2006; 26(12): 3245 - 3255.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. A. Chubykin, X. Liu, D. Comoletti, I. Tsigelny, P. Taylor, and T. C. Sudhof
Dissection of Synapse Induction by Neuroligins: EFFECT OF A NEUROLIGIN MUTATION ASSOCIATED WITH AUTISM
J. Biol. Chem., June 10, 2005; 280(23): 22365 - 22374.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
W. Zhang, A. Rohlmann, V. Sargsyan, G. Aramuni, R. E. Hammer, T. C. Sudhof, and M. Missler
Extracellular Domains of {alpha}-Neurexins Participate in Regulating Synaptic Transmission by Selectively Affecting N- and P/Q-Type Ca2+ Channels
J. Neurosci., April 27, 2005; 25(17): 4330 - 4342.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. I. Nam and L. Chen
Postsynaptic assembly induced by neurexin-neuroligin interaction and neurotransmitter
PNAS, April 26, 2005; 102(17): 6137 - 6142.
[Abstract] [Full Text] [PDF]

Home page
 Sci SignalHome page
T. Biederer
Progress from the Postsynaptic Side: Signaling in Synaptic Differentiation
Sci. Signal., March 8, 2005; 2005(274): pe9 - pe9.
[Abstract] [Full Text] [PDF]


-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help
-
Copyright 2010 by Society for Neuroscience ONLINE ISSN: 1529-2401
-