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The Journal of Neuroscience, June 1, 2005, 25(22):5333-5338; doi:10.1523/JNEUROSCI.1085-05.2005
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BRIEF COMMUNICATION
Rapid Reversible Changes in Dendritic Spine Structure In Vivo Gated by the Degree of Ischemia
Shengxiang Zhang,1,2
Jamie Boyd,4
Kerry Delaney,4 and
Timothy H. Murphy1,2,3
1Kinsmen Laboratory, Department of Psychiatry, 2Brain Research Center, and 3Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3, and 4Department of Biology, University of Victoria, Victoria, British Columbia, Canada V8W 3N5
Current therapeutic windows for effective application of thrombolytic agents are within 3-6 h of stroke. Although treatment can improve outcome, it is unclear what happens to synaptic fine structure during this critical period in vivo. The relationship between microcirculation and dendritic spine structure was determined in mouse somatosensory neurons during stroke. Spines were, on average, 13 µm from a capillary and were supplied by  100 red blood cells per second. Moderate ischemia (50% supply) did not significantly affect spines within 5 h; however, severe ischemia (<10% supply) caused a rapid loss of spine and dendrite structure within as little as 10 min. Surprisingly, if reperfusion occurred within 20-60 min, dendrite and spine structure was mostly restored. These data suggest that the basic dendritic wiring diagram remains mostly intact during moderate ischemia and that affected synapses could potentially contribute to functional recovery. With severe ischemia, markedly deformed dendritic structure can partially recover if reperfusion occurs early.
Key words: stroke; dendritic spine; plasticity; fluorescence microscopy; cerebral blood flow; dendritic arborization; glutamatergic neurons; ischemia; PSD; recovery; structural plasticity
Received March 20, 2005;
revised April 18, 2005;
accepted April 21, 2005.
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