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The Journal of Neuroscience, June 8, 2005, 25(23):5611-5622; doi:10.1523/JNEUROSCI.3972-04.2005

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Development/Plasticity/Repair
Axodendritic Contacts onto Calcium/Calmodulin-Dependent Protein Kinase Type II-Expressing Neurons in the Barn Owl Auditory Space Map

Adrian Rodriguez-Contreras, Xiao-Bo Liu, and William M. DeBello

Department of Neurobiology, Physiology, and Behavior, Center for Neuroscience, University of California, Davis, Davis, California 95616

In the owl midbrain, a map of auditory space is synthesized in the inferior colliculus (IC) and conveyed to the optic tectum (OT). Ascending auditory information courses through these structures via topographic axonal projections. Little is known about the molecular composition of projection neurons or their postsynaptic targets. To visualize axodendritic contacts between identified cell types, we used double-label immunohistochemistry, in vivo retrograde tracing, in vitro anterograde tracing, high-resolution confocal microscopy, three-dimensional reconstruction and fly-through visualization. We discovered a major class of IC neurons that strongly expressed calcium/calmodulin-dependent protein kinase type II, subunit (CaMKII). The distribution of these cells within the IC was mostly restricted to the external nucleus of the IC (ICX), in which the auditory space map is assembled. A large proportion of ICX-OT projection neurons were CaMKII positive. In addition to being the principal outputs, CaMKII cells were in direct contact with axonal boutons emanating from the main source of input to ICX, the lateral shell of the central nucleus of the inferior colliculus (ICCls). Numerous sites of putative synaptic contact were found on the somata, proximal dendrites, and distal dendrites. Double-label immunoelectron microscopy confirmed the existence of synapses between ICCls axons and the dendrites of CaMKII cells. Collectively, our data indicate that CaMKII ICX neurons are a cellular locus for the computation of auditory space-specific responses. Because the ICCls-ICX projection is physically altered during experience-dependent plasticity, these results lay the groundwork for probing microanatomical rearrangements that may underlie plasticity and learning.

Key words: convergence; dendrite; development; sound localization; synapse

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Received Sep 24, 2004; revised April 19, 2005; accepted April 22, 2005.

This article has been cited by other articles:

				G. S. Nichols and W. M. DeBello Bidirectional Regulation of the cAMP Response Element Binding Protein Encodes Spatial Map Alignment in Prism-Adapting Barn Owls J. Neurosci., October 1, 2008; 28(40): 9898 - 9909. [Abstract] [Full Text] [PDF]

				T. J. McBride, A. Rodriguez-Contreras, A. Trinh, R. Bailey, and W. M. DeBello Learning Drives Differential Clustering of Axodendritic Contacts in the Barn Owl Auditory System J. Neurosci., July 2, 2008; 28(27): 6960 - 6973. [Abstract] [Full Text] [PDF]

				Y. Gutfreund and E. I. Knudsen Adaptation in the Auditory Space Map of the Barn Owl J Neurophysiol, August 1, 2006; 96(2): 813 - 825. [Abstract] [Full Text] [PDF]

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