 |
||||
|
||||
|
||||
|
||||
The Journal of Neuroscience, June 15, 2005, 25(24):5691-5699; doi:10.1523/JNEUROSCI.1030-05.2005
Previous Article | Next Article
Development/Plasticity/Repair
Nucleokinesis in Tangentially Migrating Neurons Comprises Two Alternating Phases: Forward Migration of the Golgi/Centrosome Associated with Centrosome Splitting and Myosin Contraction at the Rear
Arnaud Bellion,2 * Jean-Pierre Baudoin,1 * Chantal Alvarez,1 Michel Bornens,3 andChristine Métin1,2 1Unité 616, Institut National de la Santé et de la Recherche Médicale, Université Paris 6, Institut Fédératif de Recherche des Sciences 70, Hôpital Pitié-Salpêtrière, 75651 Paris Cédex 13, France, 2Unité Mixte de Recherche 8542, Centre National de la Recherche Scientifique, Ecole Normale Supérieure, Equipe Régionalisation Nerveuse, 75230 Paris Cédex 05, France, and 3Unité Mixte de Recherche 144, Centre National de la Recherche Scientifique, Institut Curie, 75248 Paris Cédex 05, France
During rodent cortex development, cells born in the medial ganglionic eminence (MGE) of the basal telencephalon reach the embryonic cortex by tangential migration and differentiate as interneurons. Migrating MGE cells exhibit a saltatory progression of the nucleus and continuously extend and retract branches in their neuritic arbor. We have analyzed the migration cycle of these neurons using in vitro models.
We show that the nucleokinesis in MGE cells comprises two phases. First, cytoplasmic organelles migrate forward, and second, the nucleus translocates toward these organelles. During the first phase, a large swelling that contains the centrosome and the Golgi apparatus separates from the perinuclear compartment and moves rostrally into the leading neurite, up to 30 µm from the waiting nucleus. This long-distance migration is associated with a splitting of the centrioles that line up along a linear Golgi apparatus. It is followed by the second, dynamic phase of nuclear translocation toward the displaced centrosome and Golgi apparatus.
The forward movement of the nucleus is blocked by blebbistatin, a specific inhibitor of nonmuscle myosin II. Because myosin II accumulates at the rear of migrating MGE cells, actomyosin contraction likely plays a prominent role to drive forward translocations of the nucleus toward the centrosome. During this phase of nuclear translocation, the leading growth cone either stops migrating or divides, showing a tight correlation between leading edge movements and nuclear movements.
Key words: migration; video microscopy; cortical interneurons; centrosome; Golgi apparatus; nonmuscle myosin II
Received March 16, 2005; revised May 1, 2005; accepted May 2, 2005.
This article has been cited by other articles:
C. Metin, R. B. Vallee, P. Rakic, and P. G. Bhide
Modes and Mishaps of Neuronal Migration in the Mammalian Brain
J. Neurosci., November 12, 2008; 28(46): 11746 - 11752.
[Abstract] [Full Text] [PDF]
C. Beadle, M. C. Assanah, P. Monzo, R. Vallee, S. S. Rosenfeld, and P. Canoll
The Role of Myosin II in Glioma Invasion of the Brain
Mol. Biol. Cell, August 1, 2008; 19(8): 3357 - 3368.
[Abstract] [Full Text] [PDF]
Y. Hirota, T. Ohshima, N. Kaneko, M. Ikeda, T. Iwasato, A. B. Kulkarni, K. Mikoshiba, H. Okano, and K. Sawamoto
Cyclin-Dependent Kinase 5 Is Required for Control of Neuroblast Migration in the Postnatal Subventricular Zone
J. Neurosci., November 21, 2007; 27(47): 12829 - 12838.
[Abstract] [Full Text] [PDF]
C. H. Faux and J. G. Parnavelas
The Role of Intracellular Calcium and RhoA in Neuronal Migration
Sci. Signal., November 13, 2007; 2007(412): pe62 - pe62.
[Abstract] [Full Text] [PDF]
H. Umeshima, T. Hirano, and M. Kengaku
Microtubule-based nuclear movement occurs independently of centrosome positioning in migrating neurons
PNAS, October 9, 2007; 104(41): 16182 - 16187.
[Abstract] [Full Text] [PDF]
C. Metin, C. Alvarez, D. Moudoux, T. Vitalis, C. Pieau, and Z. Molnar
Conserved pattern of tangential neuronal migration during forebrain development
Development, August 1, 2007; 134(15): 2815 - 2827.
[Abstract] [Full Text] [PDF]
T. Ohshima, M. Hirasawa, H. Tabata, T. Mutoh, T. Adachi, H. Suzuki, K. Saruta, T. Iwasato, S. Itohara, M. Hashimoto, et al.
Cdk5 is required for multipolar-to-bipolar transition during radial neuronal migration and proper dendrite development of pyramidal neurons in the cerebral cortex
Development, June 15, 2007; 134(12): 2273 - 2282.
[Abstract] [Full Text] [PDF]
J. E. Crandall, D. M. McCarthy, K. Y. Araki, J. R. Sims, J.-Q. Ren, and P. G. Bhide
Dopamine Receptor Activation Modulates GABA Neuron Migration from the Basal Forebrain to the Cerebral Cortex
J. Neurosci., April 4, 2007; 27(14): 3813 - 3822.
[Abstract] [Full Text] [PDF]
G. Friocourt, J. S. Liu, M. Antypa, S. Rakic, C. A. Walsh, and J. G. Parnavelas
Both Doublecortin and Doublecortin-Like Kinase Play a Role in Cortical Interneuron Migration
J. Neurosci., April 4, 2007; 27(14): 3875 - 3883.
[Abstract] [Full Text] [PDF]
L. Zhou, Y. Jossin, and A. M. Goffinet
Identification of Small Molecules That Interfere with Radial Neuronal Migration and Early Cortical Plate Development
Cereb Cortex, January 1, 2007; 17(1): 211 - 220.
[Abstract] [Full Text] [PDF]
R. B. Vallee and J.-W. Tsai
The cellular roles of the lissencephaly gene LIS1, and what they tell us about brain development
Genes & Dev., June 1, 2006; 20(11): 1384 - 1393.
[Full Text] [PDF]
C. Kappeler, Y. Saillour, J.-P. Baudoin, F. P. D. Tuy, C. Alvarez, C. Houbron, P. Gaspar, G. Hamard, J. Chelly, C. Metin, et al.
Branching and nucleokinesis defects in migrating interneurons derived from doublecortin knockout mice
Hum. Mol. Genet., May 1, 2006; 15(9): 1387 - 1400.
[Abstract] [Full Text] [PDF]
B. T. Schaar and S. K. McConnell
Cytoskeletal coordination during neuronal migration
PNAS, September 20, 2005; 102(38): 13652 - 13657.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|