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The Journal of Neuroscience, July 13, 2005, 25(28):6610-6620; doi:10.1523/JNEUROSCI.5009-04.2005

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Cellular/Molecular
The Zebrafish shocked Gene Encodes a Glycine Transporter and Is Essential for the Function of Early Neural Circuits in the CNS

Wilson W. Cui,¹ Sean E. Low,² Hiromi Hirata,³ Louis Saint-Amant,³ Robert Geisler,⁴ Richard I. Hume,^2,3 and John Y. Kuwada^1,2,3

¹Cell and Molecular Biology Graduate Program, ²Neuroscience Graduate Program, and ³Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109 and ⁴Max-Planck-Institut für Entwicklungsbiologie, Abteilung III, Tübingen 72076, Germany

shocked (sho) is a zebrafish mutation that causes motor deficits attributable to CNS defects during the first2dof development. Mutant embryos display reduced spontaneous coiling of the trunk, diminished escape responses when touched, and an absence of swimming. A missense mutation in the slc6a9 gene that encodes a glycine transporter (GlyT1) was identified as the cause of the sho phenotype. Antisense knock-down of GlyT1 in wild-type embryos phenocopies sho, and injection of wild-type GlyT1 mRNA into mutants rescues them. A comparison of glycine-evoked inward currents in Xenopus oocytes expressing either the wild-type or mutant protein found that the missense mutation results in a nonfunctional transporter. glyt1 and the related glyt2 mRNAs are expressed in the hindbrain and spinal cord in nonoverlapping patterns. The fact that these regions are known to be required for generation of early locomotory behaviors suggests that the regulation of extracellular glycine levels in the CNS is important for proper function of neural networks. Furthermore, physiological analysis after manipulation of glycinergic activity in wild-type and sho embryos suggests that the mutant phenotype is attributable to elevated extracellular glycine within the CNS.

Key words: behavior; zebrafish; mutation; glycine transporter; behavior; strychnine

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Received Dec 8, 2004; revised May 31, 2005; accepted June 2, 2005.

This article has been cited by other articles:

				K. E. Epley, J. M. Urban, T. Ikenaga, and F. Ono A Modified Acetylcholine Receptor {delta}-Subunit Enables a Null Mutant to Survive Beyond Sexual Maturation J. Neurosci., December 3, 2008; 28(49): 13223 - 13231. [Abstract] [Full Text] [PDF]

				R. Mongeon, M. R. Gleason, M. A. Masino, J. R. Fetcho, G. Mandel, P. Brehm, and J. E. Dallman Synaptic Homeostasis in a Zebrafish Glial Glycine Transporter Mutant J Neurophysiol, October 1, 2008; 100(4): 1716 - 1723. [Abstract] [Full Text] [PDF]

				K. E Lewis How do genes regulate simple behaviours? Understanding how different neurons in the vertebrate spinal cord are genetically specified Phil Trans R Soc B, January 29, 2006; 361(1465): 45 - 66. [Abstract] [Full Text] [PDF]

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