QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, January 19, 2005, 25(3):558-565; doi:10.1523/JNEUROSCI.3799-04.2005

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (23) Citing Articles via Google Scholar Google Scholar Articles by Müller, A. Articles by Dietrich, D. Search for Related Content PubMed PubMed Citation Articles by Müller, A. Articles by Dietrich, D.

 Previous Article  |  Next Article 

Cellular/Molecular
Endogenous Ca²⁺ Buffer Concentration and Ca²⁺ Microdomains in Hippocampal Neurons

Andreas Müller,¹ Maria Kukley,¹ Pia Stausberg,¹ Heinz Beck,² Wolfgang Müller,³ and Dirk Dietrich¹

Departments of ¹Neurosurgery and ²Epileptology, University Clinic Bonn, D-53105 Bonn, Germany, and ³Charite, Neurowissenschaftliches Forschungszentrum, D-10117 Berlin, Germany

Ca²⁺-binding proteins are ubiquitously expressed throughout the CNS and serve as valuable immunohistochemical markers for certain types of neurons. However, the functional role of most Ca²⁺-binding proteins has to date remained obscure because their concentration in central neurons is not known. In this study, we investigate the intracellular concentration of the widely expressed Ca²⁺-binding protein calbindin-D_28k in adult hippocampal slices using patch-clamp recordings and immunohistochemistry. First, we show that calbindin-D_28k freely exchanges between patch pipette and cytoplasm during whole cell patch-clamp recordings with a time constant of 10 min. Substituting known concentrations of recombinant calbindin-D_28k in patch pipettes enabled us to determine the endogenous calbindin-D_28k concentration by postrecording immunohistochemistry. Using this calibration procedure, we find that mature granule cells (doublecortin-) contain 40 µM, and newborn granule cells (doublecortin+) contain 0-20 µM calbindin-D_28k. CA3 stratum radiatum interneurons and CA1 pyramidal cells enclose 47 and 45 µM calbindin-D_28k, respectively. Numerical simulations showed that 40 µM calbindin-D_28k is capable of tuning Ca²⁺ microdomains associated with action potentials at the mouth of single or clustered Ca²⁺ channels: calbindin-D_28k reduces the increment in free Ca²⁺ at a distance of 100 and 200 nm by 20 and 35%, respectively, and strongly accelerates the collapse of the Ca²⁺ gradient after cessation of Ca²⁺ influx. These data suggest that calbindin-D_28k equips hippocampal neurons with 160 µM mobile, high-affinity Ca²⁺-binding sites (_S 200) that slow and reduce global Ca²⁺ signals while they enhance the spatiotemporal fidelity of submicroscopic Ca²⁺ signals.

Key words: calcium; calcium binding ratio; adult neurogenesis; confocal laser scanning microscopy; buffered calcium diffusion; single compartment model

---

Received Sep 14, 2004; revised November 18, 2004; accepted November 22, 2004.

This article has been cited by other articles:

				R. C. Foehring, X. F. Zhang, J.C.F. Lee, and J. C. Callaway Endogenous Calcium Buffering Capacity of Substantia Nigral Dopamine Neurons J Neurophysiol, October 1, 2009; 102(4): 2326 - 2333. [Abstract] [Full Text] [PDF]

				T. Hori and T. Takahashi Mechanisms underlying short-term modulation of transmitter release by presynaptic depolarization J. Physiol., June 15, 2009; 587(12): 2987 - 3000. [Abstract] [Full Text] [PDF]

				G. Stocca, C. Schmidt-Hieber, and J. Bischofberger Differential dendritic Ca2+ signalling in young and mature hippocampal granule cells J. Physiol., August 15, 2008; 586(16): 3795 - 3811. [Abstract] [Full Text] [PDF]

				R. Scott, A. Ruiz, C. Henneberger, D. M. Kullmann, and D. A. Rusakov Analog Modulation of Mossy Fiber Transmission Is Uncoupled from Changes in Presynaptic Ca2+ J. Neurosci., July 30, 2008; 28(31): 7765 - 7773. [Abstract] [Full Text] [PDF]

				D. Lee, K.-H. Lee, W.-K. Ho, and S.-H. Lee Target Cell-Specific Involvement of Presynaptic Mitochondria in Post-Tetanic Potentiation at Hippocampal Mossy Fiber Synapses J. Neurosci., December 12, 2007; 27(50): 13603 - 13613. [Abstract] [Full Text] [PDF]

				H. Schmidt, S. Kunerth, C. Wilms, R. Strotmann, and J. Eilers Spino-dendritic cross-talk in rodent Purkinje neurons mediated by endogenous Ca2+-binding proteins J. Physiol., June 1, 2007; 581(2): 619 - 629. [Abstract] [Full Text] [PDF]

				A. Muller, M. Kukley, M. Uebachs, H. Beck, and D. Dietrich Nanodomains of Single Ca2+ Channels Contribute to Action Potential Repolarization in Cortical Neurons J. Neurosci., January 17, 2007; 27(3): 483 - 495. [Abstract] [Full Text] [PDF]

				R. Scott and D. A. Rusakov Main determinants of presynaptic Ca2+ dynamics at individual mossy fiber-CA3 pyramidal cell synapses. J. Neurosci., June 28, 2006; 26(26): 7071 - 7081. [Abstract] [Full Text] [PDF]

				L. Kreiner and A. Lee Endogenous and Exogenous Ca2+ Buffers Differentially Modulate Ca2+-dependent Inactivation of CaV2.1 Ca2+ Channels J. Biol. Chem., February 24, 2006; 281(8): 4691 - 4698. [Abstract] [Full Text] [PDF]

				H. Song, G. Kempermann, L. O. Wadiche, C. Zhao, A. F. Schinder, and J. Bischofberger New Neurons in the Adult Mammalian Brain: Synaptogenesis and Functional Integration J. Neurosci., November 9, 2005; 25(45): 10366 - 10368. [Full Text] [PDF]

				R. Jabs, T. Pivneva, K. Huttmann, A. Wyczynski, C. Nolte, H. Kettenmann, and C. Steinhauser Synaptic transmission onto hippocampal glial cells with hGFAP promoter activity J. Cell Sci., August 15, 2005; 118(16): 3791 - 3803. [Abstract] [Full Text] [PDF]

				D. A. Rusakov, F. Saitow, K. P. Lehre, and S. Konishi Modulation of Presynaptic Ca2+ Entry by AMPA Receptors at Individual GABAergic Synapses in the Cerebellum J. Neurosci., May 18, 2005; 25(20): 4930 - 4940. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help