 |
|||||
|
|||||
|
|||||
|
|||||
|
|||||
The Journal of Neuroscience, August 31, 2005, 25(35):7914-7923; doi:10.1523/JNEUROSCI.1471-05.2005
Previous Article | Next Article
Cellular/Molecular
Gating Reaction Mechanisms for NMDA Receptor Channels
Anthony Auerbach andYu Zhou Center for Single Molecule Biophysics and Department of Physiology and Biophysics, State University of New York, Buffalo, New York 14214
NMDA receptors (NMDARs) mediate the slow component of excitatory transmission in the CNS and play key roles in synaptic plasticity and excitotoxicity. We investigated the gating reaction mechanism of fully liganded NR1/NR2A recombinant NMDARs (expressed in Xenopus oocytes) by fitting all possible three-closed/two-open-state, noncyclic kinetic schemes to currents elicited by saturating concentrations of glutamate plus glycine. The adequacy of each scheme was assessed by maximum likelihood values and autocorrelation coefficients of single-channel currents, as well as by the predicted time courses of transient macroscopic currents. Two schemes provided the best description for NMDAR gating at both the single-channel and macroscopic levels. These two schemes had coupled open states, only one gateway between the closed and open aggregates, and at least two preopening closed states. These two models could be condensed into a cyclic reaction mechanism. Using a linear reaction scheme, the overall "gating" rates (from the initial stable closed state to the final stable open state) are 177 and 4.4 s–1.
Key words: channel; kinetics; oocyte; glutamate receptor; glutamate; NMDA receptor
Received April 14, 2005; revised July 5, 2005; accepted July 5, 2005.
This article has been cited by other articles:
C. L. Kussius, N. Kaur, and G. K. Popescu
Pregnanolone Sulfate Promotes Desensitization of Activated NMDA Receptors
J. Neurosci., May 27, 2009; 29(21): 6819 - 6827.
[Abstract] [Full Text] [PDF]
S. M. Dravid, A. Prakash, and S. F. Traynelis
Activation of recombinant NR1/NR2C NMDA receptors
J. Physiol., September 15, 2008; 586(18): 4425 - 4439.
[Abstract] [Full Text] [PDF]
M. L. Blanke and A. M. J. VanDongen
The NR1 M3 Domain Mediates Allosteric Coupling in the N-Methyl-D-aspartate Receptor
Mol. Pharmacol., August 1, 2008; 74(2): 454 - 465.
[Abstract] [Full Text] [PDF]
L. Sornarajah, O. C. Vasuta, L. Zhang, C. Sutton, B. Li, A. El-Husseini, and L. A. Raymond
NMDA Receptor Desensitization Regulated by Direct Binding to PDZ1-2 Domains of PSD-95
J Neurophysiol, June 1, 2008; 99(6): 3052 - 3062.
[Abstract] [Full Text] [PDF]
K. Erreger and S. F. Traynelis
Zinc inhibition of rat NR1/NR2A N-methyl-D-aspartate receptors
J. Physiol., February 1, 2008; 586(3): 763 - 778.
[Abstract] [Full Text] [PDF]
A. I. Sobolevsky, M. L. Prodromou, M. V. Yelshansky, and L. P. Wollmuth
Subunit-specific Contribution of Pore-forming Domains to NMDA Receptor Channel Structure and Gating
J. Gen. Physiol., June 1, 2007; 129(6): 509 - 525.
[Abstract] [Full Text] [PDF]
A.-M. L. Fay and D. Bowie
Concanavalin-A reports agonist-induced conformational changes in the intact GluR6 kainate receptor
J. Physiol., April 1, 2006; 572(1): 201 - 213.
[Abstract] [Full Text] [PDF]
G. M. C. Lema and A. Auerbach
Modes and models of GABAA receptor gating
J. Physiol., April 1, 2006; 572(1): 183 - 200.
[Abstract] [Full Text] [PDF]
S. Schorge, S. Elenes, and D. Colquhoun
Maximum likelihood fitting of single channel NMDA activity with a mechanism composed of independent dimers of subunits
J. Physiol., December 1, 2005; 569(2): 395 - 418.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|