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The Journal of Neuroscience, September 21, 2005, 25(38):8620-8626; doi:10.1523/JNEUROSCI.2225-05.2005

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Cellular/Molecular
Independent Circadian Oscillations of Period1 in Specific Brain Areas In Vivo and In Vitro

Ute Abraham,¹ Julie L. Prior,² Daniel Granados-Fuentes,¹ David R. Piwnica-Worms,² and Erik D. Herzog¹

¹Department of Biology, Washington University, St. Louis, Missouri 63130, and ²Mallinckrodt Institute of Radiology, Molecular Imaging Center and Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, Missouri 63110

Behavioral and physiological circadian rhythms in mammals are controlled by a master pacemaker in the hypothalamic suprachiasmatic nuclei (SCN). Recently, circadian oscillations of hormone secretion, clock gene expression, and electrical activity have been demonstrated in explants of other brain regions. This suggests that some extra-SCN brain regions contain a functional, SCN-independent circadian clock, but in vivo evidence for intrinsic pacemaking is still lacking. We developed a novel method to image bioluminescence in vivo from the main olfactory bulbs (OB) of intact and SCN-lesioned (SCNX) Period1::luciferase rats for 2 d in constant darkness. The OBs expressed circadian rhythms in situ with a reliable twofold increase from day to night, similar to the phase and amplitude of ex vivo rhythms. In vivo cycling persisted for at least 1 month in the absence of the SCN. To assess indirectly in vivo rhythmicity of other brain areas, we measured the phase-dependence of their in vitro rhythms on the time of surgery. Surgery reliably reset the phase of the pineal gland and vascular organ of the lamina terminalis (VOLT) harvested from SCNX rats but had little effect on the phase of the OB. We deduce that the SCN and OB contain self-sustained circadian oscillators, whereas the pineal gland and VOLT are weak oscillators that require input from the SCN to show coordinated circadian rhythms. We conclude that the mammalian brain comprises a diverse set of SCN-dependent and SCN-independent circadian oscillators.

Key words: suprachiasmatic nucleus; olfactory bulb; pineal gland; vascular organ of the lamina terminalis; bioluminescence; real-time imaging

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Received April 7, 2005; revised August 9, 2005; accepted August 9, 2005.

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