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The Journal of Neuroscience, October 12, 2005, 25(41):9515-9523; doi:10.1523/JNEUROSCI.1643-05.2005

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Cellular/Molecular
Interaction of SPIN90 with Dynamin I and Its Participation in Synaptic Vesicle Endocytosis

Yujin Kim, Sunyun Kim, Suho Lee, Sung Hyun Kim, Yoonju Kim, Zee Yong Park, Woo Keun Song, and Sunghoe Chang

Department of Life Science and Molecular Diseaome Research Center, Gwangju Institute of Science and Technology, Gwangju, South Korea 500-712

SH3 protein interacting with Nck, 90 kDa (SPIN90) is an Nck-binding protein that contains one Src homology 3 (SH3) domain, three proline-rich domains (PRDs), a serine/threonine-rich region, and a hydrophobic C-terminal region. Previously, we have shown that SPIN90 plays roles in the sarcomere assembly in cardiac muscles and in the formation of focal contacts in HeLa cells. Besides in heart, SPIN90 is also highly expressed in the brain, but its role in the neuronal system is completely unknown. Here, we found that SPIN90 is expressed in the presynaptic compartment in which it binds dynamin I, a key component of the endocytic machinery, and that it participates in synaptic vesicle endocytosis. Pull-down analysis and coimmunoprecipitation proved the associations of SPIN90 with dynamin I through SH3-PRD interaction. Overexpression of SPIN90 or knocking down SPIN90 by small interfering RNA impaired synaptic vesicle endocytosis. We further confirmed by the rescue experiments that the endocytic defects by SPIN90 expression come from its interaction with dynamin I. Exocytosis kinetics was not affected by SPIN90 expression. Together, our findings suggest that SPIN90 could modulate the interactions of dynamin I with other endocytic proteins that cooperate in the coated vesicle formation, thus regulating synaptic vesicle endocytosis.

Key words: SPIN90; dynamin I; synaptic vesicle; endocytosis; SH3; PRD

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Received April 26, 2005; revised August 27, 2005; accepted August 29, 2005.

This article has been cited by other articles:

				N. Shin, S. Lee, N. Ahn, S.-A Kim, S.-G. Ahn, Z. YongPark, and S. Chang Sorting Nexin 9 Interacts with Dynamin 1 and N-WASP and Coordinates Synaptic Vesicle Endocytosis J. Biol. Chem., September 28, 2007; 282(39): 28939 - 28950. [Abstract] [Full Text] [PDF]

				S. H. Kim, H. J. Choi, K. W. Lee, N. H. Hong, B. H. Sung, K. Y. Choi, S.-M. Kim, S. Chang, S. H. Eom, and W. K. Song Interaction of SPIN90 with syndapin is implicated in clathrin-mediated endocytic pathway in fibroblasts. Genes Cells, October 1, 2006; 11(10): 1197 - 1211. [Abstract] [Full Text] [PDF]

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