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The Journal of Neuroscience, October 26, 2005, 25(43):9836-9849; doi:10.1523/JNEUROSCI.3497-05.2005

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Cellular/Molecular
7 Neuronal Nicotinic Acetylcholine Receptors Are Negatively Regulated by Tyrosine Phosphorylation and Src-Family Kinases

Eric Charpantier,^2 * Andreas Wiesner,^1 * Kyung-Hye Huh,^1 * Roch Ogier,² Jean-Charles Hoda,² Geraldine Allaman,² Mario Raggenbass,² Dominik Feuerbach,³ Daniel Bertrand,² and Christian Fuhrer¹

¹Department of Neurochemistry, Brain Research Institute, University of Zürich, CH-8057 Zürich, Switzerland, ²Department of Neurosciences, University Medical Center, CH-1211 Geneva 4, Switzerland, and ³Novartis Institutes for BioMedical Research, CH-4002 Basel, Switzerland

Nicotine, a component of tobacco, is highly addictive but possesses beneficial properties such as cognitive improvements and memory maintenance. Involved in these processes is the neuronal nicotinic acetylcholine receptor (nAChR) 7, whose activation triggers depolarization, intracellular signaling cascades, and synaptic plasticity underlying addiction and cognition. It is therefore important to investigate intracellular mechanisms by which a cell regulates 7 nAChR activity. We have examined the role of phosphorylation by combining molecular biology, biochemistry, and electrophysiology in SH-SY5Y neuroblastoma cells, Xenopus oocytes, rat hippocampal interneurons, and neurons from the supraoptic nucleus, and we found tyrosine phosphorylation of 7 nAChRs. Tyrosine kinase inhibition by genistein decreased 7 nAChR phosphorylation but strongly increased acetylcholine-evoked currents, whereas tyrosine phosphatase inhibition by pervanadate produced opposite effects. Src-family kinases (SFKs) directly interacted with the cytoplasmic loop of 7 nAChRs and phosphorylated the receptors at the plasma membrane. SFK inhibition by PP2 [4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine] or SU6656 (2,3-dihydro-N,N-dimethyl-2-oxo-3-[(4,5,6,7-tetrahydro-1H-indol-2-yl)methylene]-1H-indole-5-sulfonamide) increased 7 nAChR-mediated responses, whereas expression of active Src reduced 7 nAChR activity. Mutant 7 nAChRs lacking cytoplasmic loop tyrosine residues because of alanine replacement of Tyr-386 and Tyr-442 were more active than wild-type receptors and insensitive to kinase or phosphatase inhibition. Because the amount of surface 7 receptors was not affected by kinase or phosphatase inhibitors, these data show that functional properties of 7 nAChRs depend on the tyrosine phosphorylation status of the receptor and are the result of a balance between SFKs and tyrosine phosphatases. These findings reveal novel regulatory mechanisms that may help to understand nicotinic receptor-dependent plasticity, addiction, and pathology.

Key words: nicotinic receptor; 7 nAChR; Src kinase; phosphorylation; phosphatase; regulation

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Received March 10, 2005; accepted September 15, 2005.

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