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The Journal of Neuroscience, November 16, 2005, 25(46):10598-10606; doi:10.1523/JNEUROSCI.2990-05.2005

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Cellular/Molecular
Binding of the Positron Emission Tomography Tracer Pittsburgh Compound-B Reflects the Amount of Amyloid- in Alzheimer's Disease Brain But Not in Transgenic Mouse Brain

William E. Klunk,¹ Brian J. Lopresti,² Milos D. Ikonomovic,³ Iliya M. Lefterov,⁴ Radosveta P. Koldamova,⁵ Eric E. Abrahamson,³ Manik L. Debnath,¹ Daniel P. Holt,² Guo-feng Huang,² Li Shao,¹ Steven T. DeKosky,³ Julie C. Price,² and Chester A. Mathis²

Departments of ¹Psychiatry, ²Radiology, ³Neurology, ⁴Environmental and Occupational Health, and ⁵Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213

During the development of in vivo amyloid imaging agents, an effort was made to use micro-positron emission tomography (PET) imaging in the presenilin-1 (PS1)/amyloid precursor protein (APP) transgenic mouse model of CNS amyloid deposition to screen new compounds and further study Pittsburgh Compound-B (PIB), a PET tracer that has been shown to be retained well in amyloid-containing areas of Alzheimer's disease (AD) brain. Unexpectedly, we saw no significant retention of PIB in this model even at 12 months of age when amyloid deposition in the PS1/APP mouse typically exceeds that seen in AD. This study describes a series of ex vivo and postmortem in vitro studies designed to explain this low retention. Ex vivo brain pharmacokinetic studies confirmed the low in vivo PIB retention observed in micro-PET experiments. In vitro binding studies showed that PS1/APP brain tissue contained less than one high-affinity (K_d = 1-2 nM) PIB binding site per 1000 molecules of amyloid- (A), whereas AD brain contained >500 PIB binding sites per 1000 molecules of A. Synthetic A closely resembled PS1/APP brain in having less than one high-affinity PIB binding site per 1000 molecules of A, although the characteristics of the few high-affinity PIB binding sites found on synthetic A were very similar to those found in AD brain. We hypothesize that differences in the time course of deposition or tissue factors present during deposition lead to differences in secondary structure between A deposited in AD brain and either synthetic A or A deposited in PS1/APP brain.

Key words: transgenic mouse; amyloid-; plaques; neuroimaging; positron emission tomography; benzothiazole

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Received July 20, 2005; revised September 23, 2005; accepted October 3, 2005.

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