QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, February 16, 2005, 25(7):1701-1710; doi:10.1523/JNEUROSCI.4756-04.2005

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (10) Citing Articles via Google Scholar Google Scholar Articles by Ohlmann, A. Articles by Tamm, E. R. Search for Related Content PubMed PubMed Citation Articles by Ohlmann, A. Articles by Tamm, E. R. Pubmed/NCBI databases Gene GEO Profiles HomoloGene UniGene Genetics Home Reference

 Previous Article  |  Next Article 

Neurobiology of Disease
Ectopic Norrin Induces Growth of Ocular Capillaries and Restores Normal Retinal Angiogenesis in Norrie Disease Mutant Mice

Andreas Ohlmann,¹ Michael Scholz,¹ Andreas Goldwich,¹ Bharesh K. Chauhan,² Kristiane Hudl,³ Anne V. Ohlmann,¹ Eberhart Zrenner,³ Wolfgang Berger,⁴ Ale Cvekl,² Mathias W. Seeliger,³ and Ernst R. Tamm¹

¹Department of Anatomy, University of Erlangen-Nürnberg, D-91054 Erlangen, Germany, ²Departments of Ophthalmology and Visual Sciences and Molecular Genetics, Albert Einstein College of Medicine, Bronx, New York 10461, ³Retinal Electrodiagnostics Research Group, University Eye Hospital Department II, D-72076 Tübingen, Germany, and ⁴Institute of Medical Genetics, Division of Medical Molecular Genetics and Gene Diagnostics, University of Zürich, CH-8603 Schwerzenbach, Switzerland

Norrie disease is an X-linked retinal dysplasia that presents with congenital blindness, sensorineural deafness, and mental retardation. Norrin, the protein product of the Norrie disease gene (NDP), is a secreted protein of unknown biochemical function. Norrie disease (Ndp^y/-) mutant mice that are deficient in norrin develop blindness, show a distinct failure in retinal angiogenesis, and completely lack the deep capillary layers of the retina. We show here that the transgenic expression of ectopic norrin under control of a lens-specific promoter restores the formation of a normal retinal vascular network in Ndp^y/- mutant mice. The improvement in structure correlates with restoration of neuronal function in the retina. In addition, lenses of transgenic mice with ectopic expression of norrin show significantly more capillaries in the hyaloid vasculature that surrounds the lens during development. In vitro, lenses of transgenic mice in coculture with microvascular endothelial cells induce proliferation of the cells. Transgenic mice with ectopic expression of norrin show more bromodeoxyuridine-labeled retinal progenitor cells at embryonic day 14.5 and thicker retinas at postnatal life than wild-type littermates, indicating a putative direct neurotrophic effect of norrin. These data provide direct evidence that norrin induces growth of ocular capillaries and that pharmacologic modulation of norrin might be used for treatment of the vascular abnormalities associated with Norrie disease or other vascular disorders of the retina.

Key words: angiogenesis; retina; VEGF; PlGF; Norrie disease; Frizzled 4

---

Received Nov 20, 2004; revised December 28, 2004; accepted December 28, 2004.

This article has been cited by other articles:

				T. N. H. Masckauchan and J. Kitajewski Wnt/Frizzled Signaling in the Vasculature: New Angiogenic Factors in Sight Physiology, June 1, 2006; 21(3): 181 - 188. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help