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The Journal of Neuroscience, March 8, 2006, 26(10):2623-2634; doi:10.1523/JNEUROSCI.4186-05.2006
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Cellular/Molecular
Gasping Activity In Vitro: A Rhythm Dependent on 5-HT2A Receptors
Andrew K. Tryba,1 Fernando Peña,2 andJan-Marino Ramirez3 1Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, 2Departamento de Farmacobiología, Cinvestav-Coapa, 14330, México D.F., México, and 3Department of Organismal Biology, The University of Chicago, Chicago, Illinois 60637
Correspondence should be addressed to Dr. Andrew K. Tryba, Department of Physiology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226. Email: atryba{at}mcw.edu
Many rhythmic behaviors are continuously modulated by endogenous peptides and amines, but whether neuromodulation is critical to the expression of a rhythmic behavior often remains unknown, particularly in mammals. Here, we address this issue in the respiratory network that was isolated in spontaneously rhythmic medullary slice preparations from mice. Under control conditions, the respiratory network generates fictive eupneic activity. We hypothesized previously that this activity depends on two types of pacemaker neurons. The bursting properties of one pacemaker rely on the persistent sodium current (INa(p)) and are insensitive to blockade of calcium channels with cadmium (CI-pacemakers), whereas bursting mechanisms of a second pacemaker are sensitive to cadmium (CS-pacemakers) and the calcium-dependent nonspecific cation current blocker flufenamic acid. During hypoxia, fictive eupneic activity is supplanted by the neural correlate of gasping, which is proposed to depend only on CI-pacemakers. Because CI-pacemakers require endogenous activation of 5-HT2A receptors, we tested the hypothesis that 5-HT2A receptor activation is critical for gasping. Here, we demonstrate that fictive gasping and CI-pacemaker bursting were selectively eliminated by the 5-HT2A receptor antagonist piperidine or ketanserin. Neither 5-HT2A antagonist eliminated bursting by CS-pacemakers and ventral respiratory group (VRG) population activity. However, this VRG activity was very different from eupneic activity. In the presence of 5-HT2A antagonists, VRG activity was eliminated by flufenamic acid and could not be reliably restored by adding substance P. These data support the hypothesis that two types of pacemaker bursting mechanisms underlie fictive eupnea, whereas only one burst mechanism is critical for gasping.
Key words: substance P; pre-Bötzinger complex; hypoglossal; rhythm generation; serotonin; 5-HT2A receptor
Received Oct. 1, 2005; revised Jan. 19, 2006; accepted Jan. 23, 2006.
Correspondence should be addressed to Dr. Andrew K. Tryba, Department of Physiology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226. Email: atryba{at}mcw.edu
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