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The Journal of Neuroscience, March 8, 2006, 26(10):2777-2787; doi:10.1523/JNEUROSCI.3420-05.2006

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Cellular/Molecular
Glial Cell Line-Derived Neurotrophic Factor-Dependent Recruitment of Ret into Lipid Rafts Enhances Signaling by Partitioning Ret from Proteasome-Dependent Degradation

Brian A. Pierchala,^1,4 Jeffrey Milbrandt,³ and Eugene M. Johnson, Jr^1,2

¹Departments of Molecular Biology and Pharmacology, ²Neurology, and ³Pathology and Immunology, Washington University School of Medicine, Saint Louis, Missouri 63110, and ⁴Department of Biological Sciences, University at Buffalo, The State University of New York, Buffalo, New York 14260-1300

Correspondence should be addressed to Brian A. Pierchala, Department of Biological Sciences, University at Buffalo, The State University of New York, 109 Cooke Hall, Buffalo, NY 14260-1300. Email: bap7{at}buffalo.edu

The receptor tyrosine kinase (RTK) Ret is activated by the formation of a complex consisting of ligands such as glial cell line-derived neurotrophic factor (GDNF) and glycerophosphatidylinositol-anchored coreceptors termed GFRs. During activation, Ret translocates into lipid rafts, which is critical for functional responses to GDNF. We found that Ret was rapidly ubiquitinated and degraded in sympathetic neurons when activated with GDNF, but, unlike other RTKs that are trafficked to lysosomes for degradation, Ret was degraded predominantly by the proteasome. After GDNF stimulation, the majority of ubiquitinated Ret was located outside of lipid rafts and Ret was lost predominantly from nonraft membrane domains. Consistent with the predominance of Ret degradation outside of rafts, disruption of lipid rafts in neurons did not alter either the GDNF-dependent ubiquitination or degradation of Ret. GDNF-mediated survival of sympathetic neurons was inhibited by lipid raft depletion, and this inhibitory effect of raft disruption on GDNF-mediated survival was reversed if Ret degradation was blocked via proteasome inhibition. Therefore, lipid rafts sequester Ret away from the degradation machinery located in nonraft membrane domains, such as Cbl family E3 ligases, thereby sustaining Ret signaling.

Key words: growth factor; signal transduction; lipid microdomains; sympathetic; neurotrophic; lysosome

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Received Aug. 15, 2005; revised Jan. 25, 2006; accepted Jan. 26, 2006.

Correspondence should be addressed to Brian A. Pierchala, Department of Biological Sciences, University at Buffalo, The State University of New York, 109 Cooke Hall, Buffalo, NY 14260-1300. Email: bap7{at}buffalo.edu

This article has been cited by other articles:

				C. C. Tsui and B. A. Pierchala CD2AP and Cbl-3/Cbl-c Constitute a Critical Checkpoint in the Regulation of Ret Signal Transduction J. Neurosci., August 27, 2008; 28(35): 8789 - 8800. [Abstract] [Full Text] [PDF]

				F. Ledda, O. Bieraugel, S. S. Fard, M. Vilar, and G. Paratcha Lrig1 Is an Endogenous Inhibitor of Ret Receptor Tyrosine Kinase Activation, Downstream Signaling, and Biological Responses to GDNF J. Neurosci., January 2, 2008; 28(1): 39 - 49. [Abstract] [Full Text] [PDF]

				S. Esseghir, S. K. Todd, T. Hunt, R. Poulsom, I. Plaza-Menacho, J. S. Reis-Filho, and C. M. Isacke A Role for Glial Cell Derived Neurotrophic Factor Induced Expression by Inflammatory Cytokines and RET/GFR{alpha}1 Receptor Up-regulation in Breast Cancer Cancer Res., December 15, 2007; 67(24): 11732 - 11741. [Abstract] [Full Text] [PDF]

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