 |
||||
|
||||
|
||||
|
||||
.gif?ad=17923&adview=true)
The Journal of Neuroscience, May 31, 2006, 26(22):5863-5871; doi:10.1523/JNEUROSCI.0182-06.2006
Previous Article | Next Article
Cellular/Molecular
Roles of the Fast-Releasing and the Slowly Releasing Vesicles in Synaptic Transmission at the Calyx of Held
Takeshi Sakaba Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, D-37077 Göttingen, Germany
Correspondence should be addressed to Takeshi Sakaba, Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Am Fassberg11, D-37077 Göttingen, Germany. Email: tsakaba{at}gwdg.de
In the calyx of Held, fast and slow components of neurotransmitter release can be distinguished during a step depolarization. The two components show different sensitivity to molecular/pharmacological manipulations. Here, their roles during a high-frequency train of action potential (AP)-like stimuli were examined by using both deconvolution of EPSCs and presynaptic capacitance measurements. During a 100 Hz train of AP-like stimuli, synchronous release showed a pronounced depression within the 20 stimuli. Asynchronous release persisted during the train, was variable in its amount, and was more prominent during a 300 Hz train. We have shown previously that slowly releasing vesicles were recruited faster than fast-releasing vesicles after depletion. By further slowing recovery of the fast-releasing vesicles by inhibiting calmodulin-dependent processes (Sakaba and Neher, 2001b), the slowly releasing vesicles were isolated during recovery from vesicle depletion. When a high-frequency train was applied, the isolated slowly releasing vesicles were released predominantly asynchronously. In contrast, synchronous release was mediated mainly by the fast-releasing vesicles. The results suggest that fast-releasing vesicles contribute mainly to synchronous release and that depletion of fast-releasing vesicles shape the synaptic depression of the synchronous phase of EPSCs, whereas slowly releasing vesicles are released mainly asynchronously during high-frequency stimulation. The latter is less subject to depression presumably because of a rapid vesicular recruitment process, which is a characteristic of this component.
Key words: synaptic transmission; synaptic vesicle release; synaptic plasticity; exocytosis; presynaptic; synaptic
Received Jan. 14, 2006; revised April 18, 2006; accepted April 18, 2006.
Correspondence should be addressed to Takeshi Sakaba, Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Am Fassberg11, D-37077 Göttingen, Germany. Email: tsakaba{at}gwdg.de
Related articles in J. Neurosci.:
- This Week in The Journal
J. Neurosci. 2006 26: i.[Full Text]
This article has been cited by other articles:
J. S. Lee, M.-H. Kim, W.-K. Ho, and S.-H. Lee
Presynaptic Release Probability and Readily Releasable Pool Size Are Regulated by Two Independent Mechanisms during Posttetanic Potentiation at the Calyx of Held Synapse
J. Neurosci., August 6, 2008; 28(32): 7945 - 7953.
[Abstract] [Full Text] [PDF]
G. Srinivasan, J. H. Kim, and H. von Gersdorff
The Pool of Fast Releasing Vesicles Is Augmented by Myosin Light Chain Kinase Inhibition at the Calyx of Held Synapse
J Neurophysiol, April 1, 2008; 99(4): 1810 - 1824.
[Abstract] [Full Text] [PDF]
D. Zikich, A. Mezer, F. Varoqueaux, A. Sheinin, H. J. Junge, E. Nachliel, R. Melamed, N. Brose, M. Gutman, and U. Ashery
Vesicle Priming and Recruitment by ubMunc13-2 Are Differentially Regulated by Calcium and Calmodulin
J. Neurosci., February 20, 2008; 28(8): 1949 - 1960.
[Abstract] [Full Text] [PDF]
X. Liu and C. Chen
Different Roles for AMPA and NMDA Receptors in Transmission at the Immature Retinogeniculate Synapse
J Neurophysiol, February 1, 2008; 99(2): 629 - 643.
[Abstract] [Full Text] [PDF]
N. Hosoi, T. Sakaba, and E. Neher
Quantitative Analysis of Calcium-Dependent Vesicle Recruitment and Its Functional Role at the Calyx of Held Synapse
J. Neurosci., December 26, 2007; 27(52): 14286 - 14298.
[Abstract] [Full Text] [PDF]
R. Renden and H. von Gersdorff
Synaptic Vesicle Endocytosis at a CNS Nerve Terminal: Faster Kinetics at Physiological Temperatures and Increased Endocytotic Capacity During Maturation
J Neurophysiol, December 1, 2007; 98(6): 3349 - 3359.
[Abstract] [Full Text] [PDF]
A. Neef, D. Khimich, P. Pirih, D. Riedel, F. Wolf, and T. Moser
Probing the Mechanism of Exocytosis at the Hair Cell Ribbon Synapse
J. Neurosci., November 21, 2007; 27(47): 12933 - 12944.
[Abstract] [Full Text] [PDF]
M. J. Fedchyshyn and L.-Y. Wang
Activity-dependent changes in temporal components of neurotransmission at the juvenile mouse calyx of Held synapse
J. Physiol., June 1, 2007; 581(2): 581 - 602.
[Abstract] [Full Text] [PDF]
R. L. P. Habets and J. G. G. Borst
Dynamics of the readily releasable pool during post-tetanic potentiation in the rat calyx of Held synapse
J. Physiol., June 1, 2007; 581(2): 467 - 478.
[Abstract] [Full Text] [PDF]
M. Wolfel, X. Lou, and R. Schneggenburger
A Mechanism Intrinsic to the Vesicle Fusion Machinery Determines Fast and Slow Transmitter Release at a Large CNS Synapse
J. Neurosci., March 21, 2007; 27(12): 3198 - 3210.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|