QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, July 26, 2006, 26(30):7849-7859; doi:10.1523/JNEUROSCI.1526-06.2006

This Article Full Text Full Text (PDF) Supplemental data Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (11) Citing Articles via Google Scholar Google Scholar Articles by Apostolova, I. Articles by Schachner, M. Search for Related Content PubMed PubMed Citation Articles by Apostolova, I. Articles by Schachner, M.

 Previous Article  |  Next Article 

Neurobiology of Disease
Tenascin-R Restricts Posttraumatic Remodeling of Motoneuron Innervation and Functional Recovery after Spinal Cord Injury in Adult Mice

Ivayla Apostolova, ^* Andrey Irintchev, ^* and Melitta Schachner

Zentrum für Molekulare Neurobiologie, Universität Hamburg, D-20246 Hamburg, Germany

Correspondence should be addressed to Melitta Schachner or Andrey Irintchev, Zentrum für Molekulare Neurobiologie, Universität Hamburg, Martinistrasse 52, D-20246 Hamburg, Germany. melitta.schachner{at}zmnh.uni-hamburg.de or andrey.irintchev{at}zmnh.uni-hamburg.de

Tenascin-R (TNR) is an extracellular glycoprotein in the CNS implicated in neural development and plasticity. Its repellent properties for growing axons in a choice situation with a conducive substrate in vitro have indicated that TNR may impede regeneration in the adult mammalian CNS. Here we tested whether constitutive lack of TNR has beneficial impacts on recovery from spinal cord injury in adult mice. Using the Basso, Beattie, Bresnahan (BBB) locomotor rating scale, we found that open-field locomotion in TNR-deficient (TNR^–/–) mice recovered better that in wild-type (TNR^+/+) littermates after compression of the thoracic spinal cord. We also designed, validated, and applied a motion analysis approach allowing numerical assessment of motor functions. We found, in agreement with the BBB score, that functions requiring low levels of supraspinal control such as plantar stepping improved more in TNR^–/– mice. This was not the case for motor tasks demanding precision such as ladder climbing. Morphological analyses revealed no evidence that improved recovery of some functions in the mutant mice were attributable to enhanced tissue sparing or axonal regrowth. Estimates of perisomatic puncta revealed reduced innervation by cholinergic and GABAergic terminals around motoneurons in intact TNR^–/– compared with TNR^+/+ mice. Relative to nonlesioned animals, spinal cord repair was associated with increase in GABAergic and decrease of glutamatergic puncta in TNR^–/– but not in TNR^+/+ mice. Our results suggest that TNR restricts functional recovery by limiting posttraumatic remodeling of synapses around motoneuronal cell bodies where TNR is normally expressed in perineuronal nets.

Key words: functional recovery; motion analysis; mouse; tenascin-R; spinal cord injury; synaptic plasticity

---

Received April 9, 2006; revised May 23, 2006; accepted June 20, 2006.

Correspondence should be addressed to Melitta Schachner or Andrey Irintchev, Zentrum für Molekulare Neurobiologie, Universität Hamburg, Martinistrasse 52, D-20246 Hamburg, Germany. melitta.schachner{at}zmnh.uni-hamburg.de or andrey.irintchev{at}zmnh.uni-hamburg.de

This article has been cited by other articles:

				H. Liao, W. Huang, M. Schachner, Y. Guan, J. Guo, J. Yan, J. Qin, X. Bai, and L. Zhang {beta} 1 Integrin-mediated Effects of Tenascin-R Domains EGFL and FN6-8 on Neural Stem/Progenitor Cell Proliferation and Differentiation in Vitro J. Biol. Chem., October 10, 2008; 283(41): 27927 - 27936. [Abstract] [Full Text] [PDF]

				G. Hargus, Y. Cui, J.-S. Schmid, J. Xu, M. Glatzel, M. Schachner, and C. Bernreuther Tenascin-R Promotes Neuronal Differentiation of Embryonic Stem Cells and Recruitment of Host-Derived Neural Precursor Cells After Excitotoxic Lesion of the Mouse Striatum Stem Cells, August 1, 2008; 26(8): 1973 - 1984. [Abstract] [Full Text] [PDF]

				I. Jakovcevski, J. Wu, N. Karl, I. Leshchyns'ka, V. Sytnyk, J. Chen, A. Irintchev, and M. Schachner Glial Scar Expression of CHL1, the Close Homolog of the Adhesion Molecule L1, Limits Recovery after Spinal Cord Injury J. Neurosci., July 4, 2007; 27(27): 7222 - 7233. [Abstract] [Full Text] [PDF]

				J. Chen, J. Wu, I. Apostolova, M. Skup, A. Irintchev, S. Kugler, and M. Schachner Adeno-associated virus-mediated L1 expression promotes functional recovery after spinal cord injury Brain, April 1, 2007; 130(4): 954 - 969. [Abstract] [Full Text] [PDF]

				C. M. Galtrey, R. A. Asher, F. Nothias, and J. W. Fawcett Promoting plasticity in the spinal cord with chondroitinase improves functional recovery after peripheral nerve repair Brain, April 1, 2007; 130(4): 926 - 939. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help